Rabbit Recombinant Monoclonal ZEB1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--western-blot-img100320.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24868.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986)")
![Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--immunocytochemistry-immunofluorescence-img21839.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24103.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986)")
![Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--flow-cytometry-intracellular-img414470.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (AB228986)")
Publications
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- Cancer cell international 20:5692020LINC00152 upregulates ZEB1 expression and enhances epithelial-mesenchymal transition and oxaliplatin resistance in esophageal cancer by interacting with EZH2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuyao Zhang et. al.PubMed 33292221
- Cell biology international 44:2438-24492020ACTA2-AS1 suppresses lung adenocarcinoma progression via sequestering miR-378a-3p and miR-4428 to elevate SOX7 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKangtai Ying,Ling Wang,Guangyan Long,Chan Lian,Zhe Chen,Wei LinPubMed 32808728
- OncoTargets and therapy 13:1159-11702020LncRNA PCAT6 Accelerates the Progression and Chemoresistance of Cervical Cancer Through Up-Regulating ZEB1 by Sponging miR-543.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongping Ma et. al.PubMed 32103984
- Cells 9:2019Disruption of Cancer Metabolic SREBP1/miR-142-5p Suppresses Epithelial-Mesenchymal Transition and Stemness in Esophageal Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Ming Huang et. al.PubMed 31861383
- BMC cancer 19:11572019LINC01128 expedites cervical cancer progression by regulating miR-383-5p/SFN axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Hu et. al.PubMed 31779593
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
7 products for Alternative Product
2 products for Alternative Version
Target data
Function
Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).
Alternative names
AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8
Recommended products
Rabbit Recombinant Monoclonal ZEB1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17375
- Purification technique
- Affinity purification Protein A
- Specificity
Expression levels of the target protein vary with sample type and some optimisation may be required.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab228986 is the carrier-free version of Anti-ZEB1 antibody [EPR17375] ab203829.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.
- Biological function summary
ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.
- Pathways
ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.
- Associated diseases and disorders
The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
![Western blot - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--western-blot-img100320.jpg "Western blot - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)")
Western blot - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)
**Lanes 1 - 3:** Merged signal (red and green). Green - Anti-ZEB1 antibody [EPR17375] ab203829 observed at 200 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-ZEB1 antibody [EPR17375] ab203829 was shown to specifically react with ZEB1 in wild-type HAP1 cells as signal was lost in ZEB1 knockout cells. Wild-type and ZEB1 knockout samples were subjected to SDS-PAGE. Anti-ZEB1 antibody [EPR17375] ab203829 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZEB1 antibody [EPR17375] ab203829).Lane 1: Western blot - Anti-ZEB1 antibody [EPR17375] (Anti-ZEB1 antibody [EPR17375] ab203829)
Lanes 2 - 3: Western blot - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: ZEB1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Predicted band size: 124 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24868.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)
This IHC data was generated using the same anti-ZEB1 antibody clone, EPR17375, in a different buffer formulation (cat# Anti-ZEB1 antibody [EPR17375] ab203829).
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling ZEB1 with Anti-ZEB1 antibody [EPR17375] ab203829 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--immunocytochemistry-immunofluorescence-img21839.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)")
Immunocytochemistry/ Immunofluorescence - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)
This ICC data was generated using the same anti-ZEB1 antibody clone, EPR17375, in a different buffer formulation (cat# Anti-ZEB1 antibody [EPR17375] ab203829).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ZEB1 with Anti-ZEB1 antibody [EPR17375] ab203829 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-ZEB1 antibody [EPR17375] ab203829 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24103.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ZEB1 with Anti-ZEB1 antibody [EPR17375] ab203829 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on Human breast carcinoma tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZEB1 antibody [EPR17375] ab203829).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--flow-cytometry-intracellular-img414470.jpg "Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)")
Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)
Intracellular Flow Cytometry analysis of HeLa cells labelling ZEB1 with Anti-ZEB1 antibody [EPR17375] ab203829 at 1/1000 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor� 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZEB1 antibody [EPR17375] ab203829).
![Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--flow-cytometry-intracellular-img414904.jpg "Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)")
Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)
Intracellular flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ZEB1 with purified Anti-ZEB1 antibody [EPR17375] ab203829 at 1/50 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ZEB1 antibody [EPR17375] ab203829).
![Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986), expandable thumbnail](https://content.abcam.com/products/images/zeb1-antibody-epr17375-bsa-and-azide-free-ab228986--flow-cytometry-intracellular-img414904.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] - BSA and Azide free (ab228986)")
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