Anti-ZEB1 antibody [EPR26184-63] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR26184-63] - BSA and Azide free (AB303481)

This data was developed using ab303480, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded A wild-type HAP1 (H tissue labeling ZEB1 with ab303480 at 1/4000 dilution (0.13 μg/mL), followed by a ready to use LeicaDS9800 dilution (BOND™ Polymer Refine Detection). Nuclear staining on (A) Wild-type HAP1 cell pellet, no staining is observed on (B) ZEB1 knockout HAP1 cell pellet. The section was incubated with ab303480 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 dilution (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR26184-63] - BSA and Azide free (AB303481)

This data was developed using ab303480, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling ZEB1 with ab303480 at 1/8000 (0.065 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human astrocytoma (PMID : 28945795). The section was incubated with ab303480 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR26184-63] - BSA and Azide free (AB303481)

This data was developed using ab303480, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling ZEB1 with ab303480 at 1/8000 (0.065 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on stromal cells of human colon (PMID : 26057751). The section was incubated with ab303480 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR26184-63] - BSA and Azide free (AB303481)

This data was developed using ab303480, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ZEB1 with ab303480 at 1/8000 (0.065 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on rat cerebrum. The section was incubated with ab303480 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [EPR26184-63] - BSA and Azide free (AB303481)

This data was developed using ab303480, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling ZEB1 with ab303480 at 1/8000 (0.065 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on stromal cells of mouse colon (PMID : 31366457). The section was incubated with ab303480 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• Dot

Supplier Data

Dot Blot - Anti-ZEB1 antibody [EPR26184-63] - BSA and Azide free (AB303481)

This data was developed using ab303480, the same antibody clone in a different buffer formulation. Dot blot analysis of ZEB1 using ab303480 at 1 : 1000 (0.52 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST