AB180905

Anti-ZEB1 antibody [OTI3G6] - N-terminal

Name: Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905) | Abcam
Brand: Abcam Limited
SKU: AB180905
Price: 545 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(23 Publications)

Mouse Monoclonal ZEB1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB1 aa 1-350.

View Alternative Names

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast adenocarcinoma tissue labeling ZEB1 with ab180905. Heat mediated antigen retrieval was performed using 10mM citrate buffer pH 6 for 3 minutes at 120°C.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue labeling ZEB1 with ab180905. Heat mediated antigen retrieval was performed using 10mM citrate buffer pH 6 for 3 minutes at 120°C.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ZEB1 with ab180905 at 1/150 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling ZEB1 with ab180905 at 1/150 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling ZEB1 with ab180905 at 1/150 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling ZEB1 with ab180905 at 1/150 dilution.

Supplier Data

Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

All lanes:

Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905) at 1/2000 dilution

Lane 1:

HEK293T cells transfected with plasmids overexpressing either ZEB2

Lane 2:

HEK293T cells transfected with plasmids overexpressing either ZEB1

Predicted band size: 123.9 kDa

false

Supplier Data

Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

Lanes 1 - 3:

Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905) at 1/500 dilution

Lane 1:

HEK293T cells transfected with empty plasmid at 15 µg

Lane 2:

HEK293T cells transfected with human ZEB1 plasmid at 15 µg

Lane 3:

HEK293T cells transfected with mouse ZEB1 plasmid at 15 µg

Predicted band size: 123.9 kDa

false

Supplier Data

Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905)

All lanes:

Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905) at 1/500 dilution

All lanes:

A549 cells at 50 µg

Predicted band size: 123.9 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3G6

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZEB1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P37275

Specificity

Does not cross react with ZEB2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": " for 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Dilute in PBS (pH7.3) if necessary.

The clone number has been updated from 3G6 to OTI3G6, both clone numbers name the same clone.

This product was changed from ascites to tissue culture supernatant on 6^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.

Biological function summary

ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.

Pathways

ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.

The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).

See full target information ZEB1

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 29:691-711 PubMed36550594

2022

LncRNA LINC01018/miR-942-5p/KNG1 axis regulates the malignant development of glioma in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfang Xu,Jianli Wang,Mingfei Zhao,Chenguang Li,Shen Hong,Jianmin Zhang

Cell death & disease 13:723 PubMed35985997

2022

METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an mA-YTHDF2-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaren Li,Hanyu Yao,Jin Huang,Chao Li,Yichuan Zhang,Ran Xu,Zhenting Wang,Zhi Long,Jin Tang,Long Wang

Molecular and clinical oncology 17:123 PubMed35911664

2022

ZEB1 induces N-cadherin expression in human glioblastoma and may alter patient survival.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Gött,Jasmin Nagl,Frederike Hagedorn,Samuel Thomas,Frank P Schwarm,Eberhard Uhl,Malgorzata A Kolodziej

NPJ precision oncology 6:5 PubMed35042943

2022

HER3 activation contributes toward the emergence of ALK inhibitor-tolerant cells in ALK-rearranged lung cancer with mesenchymal features.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Tanimura,Tadaaki Yamada,Koutaroh Okada,Kunihiro Nakai,Mano Horinaka,Yuki Katayama,Kenji Morimoto,Yuri Ogura,Takayuki Takeda,Shinsuke Shiotsu,Kosuke Ichikawa,Satoshi Watanabe,Yoshie Morimoto,Masahiro Iwasaku,Yoshiko Kaneko,Junji Uchino,Hirokazu Taniguchi,Kazue Yoneda,Satoaki Matoba,Toshiyuki Sakai,Hisanori Uehara,Seiji Yano,Tetsuro Kusaba,Ryohei Katayama,Koichi Takayama

Cancers 13: PubMed34680248

2021

Impact of Epithelial-Mesenchymal Transition on the Immune Landscape in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima-Zohra Khadri,Marianne Samir Makboul Issac,Louis Arthur Gaboury

Medicine 100:e25028 PubMed33761659

2021

Clinicopathological significance of SOX4 and epithelial-mesenchymal transition markers in patients with laryngeal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjie Zhang,Hui Li,Yuefeng Han,Mengjun Wang,Junjie Zhang,Shiyin Ma

International journal of clinical and experimental 14:9-23 PubMed33532019

2021

ZEB1 promotes colorectal cancer cell invasion and disease progression by enhanced LOXL2 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wang,Guiyin Sun,Chunfang Peng,Jiangyan Chen,Jin Quan,Chunrong Wu,Xiaojuan Lian,Weijun Tang,Debing Xiang

Journal of cellular and molecular medicine : PubMed33128348

2020

20(S)-Protopanaxadiol inhibits epithelial-mesenchymal transition by promoting retinoid X receptor alpha in human colorectal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyuan Lu,Hongyan Liu,Wenwen Fu,Yuchen Wang,Jianan Geng,Yaozhen Wang,Xiaofeng Yu,Quan Wang,Huali Xu,Dayun Sui

Frontiers in pharmacology 11:666 PubMed32528278

2020

Obg-Like ATPase 1 Enhances Chemoresistance of Breast Cancer Activation of TGF-β/Smad Axis Cascades.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhou Liu,Xiaoyu Miao,Bowen Xiao,Jing Huang,Xufeng Tao,Jiong Zhang,Hua Zhao,Yue Pan,Hongwei Wang,Ge Gao,Gary Guishan Xiao

International journal of oncology 56:1455-1467 PubMed32236578

2020

Long non‑coding RNA ZEB1‑AS1 predicts a poor prognosis and promotes cancer progression through the miR‑200a/ZEB1 signaling pathway in intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingwen Jiao,Shanglei Ning,Jingbo Chen,Lili Chen,Meng Jiao,Zhonghui Cui,Lingyu Guo,Wentao Mu,Hui Yang

View all publications

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