Name: Anti-ZEB1 antibody [OTI3G6] - N-terminal
SKU: ab180905
Rating: 4 (3 reviews)

Mouse Monoclonal ZEB1 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB1 aa 1-350.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (AB180905), expandable thumbnail

Publications

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human ZEB1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P37275

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P
Human	Expected	Tested
Chicken	Predicted	Predicted

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info for 1/2000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Chicken	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/150	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Chicken	Dilution info -	Notes -

Associated Products

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Target data

See full target information ZEB1

Function

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). In the absence of TGFB1, acts as a repressor of COL1A2 transcription via binding to the E-box in the upstream enhancer region (By similarity).

Alternative names

AREB6, TCF8, ZEB1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human ZEB1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link P37275
Clone number: OTI3G6
Purification technique: Affinity purification Protein A
Specificity: Does not cross react with ZEB2.
Concentration
Purification notes: Purified from TCS

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Dilute in PBS (pH7.3) if necessary.

The clone number has been updated from 3G6 to OTI3G6, both clone numbers name the same clone.

This product was changed from ascites to tissue culture supernatant on 6^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZEB1 also known as δEF1 is a zinc finger E-box-binding homeobox protein with a molecular weight of approximately 124 kDa. It acts as a transcription factor and plays an important role in the regulation of gene expression by binding to specific DNA sequences. ZEB1 is mainly expressed in epithelial and mesenchymal cells and is involved in a variety of developmental processes during embryogenesis. Its broad expression pattern reflects its versatile function in different tissues and cell types.

Biological function summary

ZEB1 modulates cellular differentiation and identity by repressing and activating target genes. This protein is particularly notable for its involvement in the epithelial-to-mesenchymal transition (EMT) an important biological process for development and wound healing. ZEB1 doesn't act alone; it forms complexes with other transcription factors and co-repressors such as SMAD and CtBP enhancing its regulatory reach. Through these interactions ZEB1 influences cell adhesion molecules and cytoskeletal components facilitating cell motility and invasion.

Pathways

ZEB1 is integral to the TGF-β signaling pathway influencing cellular responses to growth factors. It also intersects with the Wnt signaling pathway linking it to cellular proliferation and differentiation. Through these pathways ZEB1 interacts with other significant proteins including β-catenin and SMAD proteins which collaboratively regulate gene expression that affects cell phenotype and behavior.

Associated diseases and disorders

The dysregulation of ZEB1 is associated with a variety of pathological conditions including cancer and fibrotic diseases. In cancer ZEB1 contributes to tumor progression and metastasis by promoting EMT a process that enables epithelial cells to acquire mesenchymal traits enhancing their migratory capabilities. This mechanism links ZEB1 to the expression of vimentin and the suppression of E-cadherin important markers in cancer prognosis. Additionally in fibrotic disorders ZEB1's interaction with TGF-β signaling promotes pathological tissue remodeling impacting fibrosis development in organs like the liver and lungs.

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9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue labeling ZEB1 with ab180905. Heat mediated antigen retrieval was performed using 10mM citrate buffer pH 6 for 3 minutes at 120°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast adenocarcinoma tissue labeling ZEB1 with ab180905. Heat mediated antigen retrieval was performed using 10mM citrate buffer pH 6 for 3 minutes at 120°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling ZEB1 with ab180905 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling ZEB1 with ab180905 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling ZEB1 with ab180905 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ZEB1 with ab180905 at 1/150 dilution.
Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
All lanes: Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905) at 1/2000 dilution
Lane 1: HEK293T cells transfected with plasmids overexpressing either ZEB2
Lane 2: HEK293T cells transfected with plasmids overexpressing either ZEB1
Predicted band size: 123.9 kDa
Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Lanes 1 - 3: Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905) at 1/500 dilution
Lane 1: HEK293T cells transfected with empty plasmid at 15 µg
Lane 2: HEK293T cells transfected with human ZEB1 plasmid at 15 µg
Lane 3: HEK293T cells transfected with mouse ZEB1 plasmid at 15 µg
Predicted band size: 123.9 kDa
Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
All lanes: Western blot - Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905) at 1/500 dilution
All lanes: A549 cells at 50 µg
Predicted band size: 123.9 kDa