AB138222

Anti-ZEB2 antibody

Name: Anti-ZEB2 antibody (ab138222) | Abcam
Brand: Abcam Limited
SKU: AB138222
Rating: 4 (7 reviews)

(7 Reviews)

(56 Publications)

Rabbit Polyclonal ZEB2 antibody. Suitable for WB and reacts with Human samples. Cited in 56 publications. Immunogen corresponding to Synthetic Peptide within Human ZEB2.

View Alternative Names

KIAA0569, SIP1, ZFHX1B, ZFX1B, HRIHFB2411, ZEB2, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b, SMADIP1

1 Images

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Western blot - Anti-ZEB2 antibody (AB138222)

Secondary antibody - goat anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-ZEB2 antibody (ab138222) at 1/500 dilution

All lanes:

HepG2 cell lysate at 30 µg

Predicted band size: 136 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human ZEB2. The exact immunogen used to generate this antibody is proprietary information.

O60315

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab138222 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZEB2 also known as SIP1 (Smad-interacting protein 1) is a zinc finger E-box-binding homeobox 2 protein. It has a mass of approximately 150 kDa. ZEB2 functions as a transcriptional repressor in cellular processes. This protein can bind to E-box sequences in DNA regulating gene expression by repressing transcription. ZEB2 expresses mainly in neural crest-derived tissues the central nervous system and the developing heart and plays an important role in these areas.

Biological function summary

ZEB2 influences epithelial-mesenchymal transition (EMT) and acts as a regulator in developmental processes. It is part of a protein complex with Smad proteins which are key transducers of the transforming growth factor-beta (TGF-β) signaling pathway. ZEB2 modulates the expression of genes involved in differentiation proliferation and cell migration which are essential for tissue development and response to environmental cues.

Pathways

ZEB2 plays roles in the TGF-β and Wnt signaling pathways. It interacts with other proteins such as Smad2/3 in the TGF-β pathway to control gene expression related to EMT and cell differentiation. In the Wnt signaling pathway ZEB2 influences the regulation of genes that mediate cell proliferation and adhesion. These pathways are vital for normal development and homeostasis making ZEB2 an important player in cellular function.

ZEB2 has links to Mowat-Wilson syndrome and cancer progression. In Mowat-Wilson syndrome mutations in the ZEB2 gene lead to neurodevelopmental abnormalities. ZEB2 also contributes to cancer by facilitating EMT which is involved in tumor invasion and metastasis. It connects with other EMT-related proteins such as Snail and Slug in cancer contexts emphasizing its importance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed : 16061479, PubMed : 20516212). Represses transcription of E-cadherin (PubMed : 16061479). Represses expression of MEOX2 (PubMed : 20516212).

See full target information ZEB2

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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:409 PubMed39747638

2025

A multicenter study on TROP2 as a potential targeted therapy for extramammary Paget disease in Japan.

Applications

Unspecified application

Species

Unspecified reactive species

Takamichi Ito,Yuka Tanaka,Dai Ogata,Haruto Nishida,Tatsushi Shiomi,Ryo Tanaka,Aya Kawaguchi,Azusa Miyashita,Satoshi Fukushima,Naoko Shojiguchi,Hiroyuki Goto,Yaei Togawa,Takahiro Kiyohara,Yoshinao Oda,Takeshi Nakahara

EMBO reports 25:832-852 PubMed38191874

2024

BRD4 isoforms have distinct roles in tumour progression and metastasis in rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dipanwita Das,Jia Yu Leung,Shivaranjani Balamurugan,Vinay Tergaonkar,Amos Hong Pheng Loh,Cheng-Ming Chiang,Reshma Taneja

EMBO molecular medicine 15:e17836 PubMed37766669

2023

Propionate reinforces epithelial identity and reduces aggressiveness of lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh Ramesh,Paradesi Naidu Gollavilli,Luisa Pinna,Mohammad Aarif Siddiqui,Adriana Martinez Turtos,Francesca Napoli,Yasmin Antonelli,Aldo Leal-Egaña,Jesper Foged Havelund,Simon Toftholm Jakobsen,Elisa Le Boiteux,Marco Volante,Nils Joakim Faergeman,Ole N Jensen,Rasmus Siersbaek,Kumar Somyajit,Paolo Ceppi

ACS applied materials & interfaces 15:38323-38334 PubMed37549382

2023

Delivery of miR-200c-3p Using Tumor-Targeted Mesoporous Silica Nanoparticles for Breast Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Iris Garrido-Cano,Anna Adam-Artigues,Ana Lameirinhas,Juan F Blandez,Vicente Candela-Noguera,Ana Lluch,Begoña Bermejo,Felix Sancenón,Juan Miguel Cejalvo,Ramón Martínez-Máñez,Pilar Eroles

Behavioural neurology 2023:4190849 PubMed36762036

2023

Long Non-coding RNA KTN1-AS1 Targets miR-505 to Promote Glioblastoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Guo,Lingling Fang,Mingjian Li,Aizheng Li,Na Liu

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 55:e12206 PubMed36629522

2023

Immune suppressive signaling regulated by latent transforming growth factor beta binding protein 1 promotes metastasis in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Gu,Wei Wang,Changdong Sun,Li Ding,Li Li,Peng Shu,Jun Xu

Oxidative medicine and cellular longevity 2022:2521951 PubMed36193069

2022

Circ_0000189 Promotes the Malignancy of Glioma Cells via Regulating miR-192-5p-ZEB2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yang,Guoqiang Hou,Hongjin Chen,Weilin Chen,Jianwei Ge

Cancers 14: PubMed35884488

2022

ZEB2/TWIST1/PRMT5/NuRD Multicomplex Contributes to the Epigenetic Regulation of EMT and Metastasis in Colorectal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yayuan Zheng,Mingrui Dai,Yue Dong,Hanqiao Yu,Tianfu Liu,Xuejian Feng,Bin Yu,Haihong Zhang,Jiaxin Wu,Wei Kong,Xianghui Yu,Hui Wu

International journal of biological sciences 18:2186-2201 PubMed35342335

2022

SENP1 promotes triple-negative breast cancer invasion and metastasis via enhancing CSN5 transcription mediated by GATA1 deSUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchang Gao,Rongrong Wang,Jianjing Liu,Ke Zhao,Xiaolong Qian,Xianghui He,Hong Liu

Drug development research 83:432-446 PubMed34636432

2021

Paeonol inhibits human lung cancer cell viability and metastasis in vitro via miR-126-5p/ZEB2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Lv,Shibing Zhu,Huiping Chen,Ying Xu,Qingyu Su,Guofen Yu,Wei Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZEB2 antibody

Western blot - Anti-ZEB2 antibody (AB138222)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (56)

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