AB223688

Anti-ZEB2 antibody

Name: Anti-ZEB2 antibody (ab223688) | Abcam
Brand: Abcam Limited
SKU: AB223688

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(8 Publications)

Rabbit Polyclonal ZEB2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB2 aa 700 to C-terminus.

View Alternative Names

KIAA0569, SIP1, ZFHX1B, ZFX1B, HRIHFB2411, ZEB2, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b, SMADIP1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Paraffin-embedded human cerebral cortex tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Paraffin-embedded human pancreas tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis. Negative control - no staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Paraffin-embedded human glioma tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Paraffin-embedded human colon tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ZEB2 antibody (AB223688)

All lanes:

Western blot - Anti-ZEB2 antibody (ab223688) at 1/100 dilution

All lanes:

HEL (human bone marrow erythroleukemia cell line) cell lysate

Predicted band size: 136 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZEB2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60315

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZEB2 also known as SIP1 (Smad-interacting protein 1) is a zinc finger E-box-binding homeobox 2 protein. It has a mass of approximately 150 kDa. ZEB2 functions as a transcriptional repressor in cellular processes. This protein can bind to E-box sequences in DNA regulating gene expression by repressing transcription. ZEB2 expresses mainly in neural crest-derived tissues the central nervous system and the developing heart and plays an important role in these areas.

Biological function summary

ZEB2 influences epithelial-mesenchymal transition (EMT) and acts as a regulator in developmental processes. It is part of a protein complex with Smad proteins which are key transducers of the transforming growth factor-beta (TGF-β) signaling pathway. ZEB2 modulates the expression of genes involved in differentiation proliferation and cell migration which are essential for tissue development and response to environmental cues.

Pathways

ZEB2 plays roles in the TGF-β and Wnt signaling pathways. It interacts with other proteins such as Smad2/3 in the TGF-β pathway to control gene expression related to EMT and cell differentiation. In the Wnt signaling pathway ZEB2 influences the regulation of genes that mediate cell proliferation and adhesion. These pathways are vital for normal development and homeostasis making ZEB2 an important player in cellular function.

ZEB2 has links to Mowat-Wilson syndrome and cancer progression. In Mowat-Wilson syndrome mutations in the ZEB2 gene lead to neurodevelopmental abnormalities. ZEB2 also contributes to cancer by facilitating EMT which is involved in tumor invasion and metastasis. It connects with other EMT-related proteins such as Snail and Slug in cancer contexts emphasizing its importance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed : 16061479, PubMed : 20516212). Represses transcription of E-cadherin (PubMed : 16061479). Represses expression of MEOX2 (PubMed : 20516212).

See full target information ZEB2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869272

2025

Upregulation of and Suggests Reversal Towards Epithelial State in Venous Tumour Thrombus of Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Čugura,Emanuela Boštjančič,Jera Jeruc

Neoplasia (New York, N.Y.) 49:100972 PubMed38237535

2024

AEBP1 promotes papillary thyroid cancer progression by activating BMP4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Gaoda Ju,Tao Xing,Miaomiao Xu,Xin Zhang,Yuqing Sun,Zhuanzhuan Mu,Di Sun,Sen Miao,Li Li,Jun Liang,Yansong Lin

BioMed research international 2022:7850658 PubMed35865664

2022

SLC6A14 Depletion Contributes to Amino Acid Starvation to Suppress EMT-Induced Metastasis in Gastric Cancer by Perturbing the PI3K/AKT/mTORC1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qie Guo,Wen Xu,Xiao Li,Jia-Lin Sun,Xiao-Ce Gu,Fan-Bo Jing

Cell death & disease 13:417 PubMed35487888

2022

ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Ji Lim,Jae-Hyeok Kang,Yeon-Ju Kim,Seungmo Kim,Su-Jae Lee

Frontiers in pharmacology 12:727916 PubMed34421621

2021

Puerarin Prevents Acute Liver Injury Inhibiting Inflammatory Responses and ZEB2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Junfa Yang,Maomao Wu,Hui Fang,Yue Su,Lingling Zhang,Huan Zhou

Cell cycle (Georgetown, Tex.) 20:1692-1707 PubMed34334113

2021

ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Qingbao Guo,Manli Xie,Miao Guo,Feiping Yan,Lihong Li,Rui Liu

International journal of molecular medicine 46:2069-2077 PubMed33125117

2020

Hesperidin administration suppresses the proliferation of lung cancer cells by promoting apoptosis via targeting the miR‑132/ZEB2 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Song Tan,Lingling Dai,Pengcheng Tan,Wei Liu,Yuejun Mu,Jinguo Wang,Xiaoming Huang,Aihua Hou

Molecular medicine reports 20:1672-1682 PubMed31257481

2019

MicroRNA‑30a regulates cell proliferation, migration, invasion and apoptosis in human nasopharyngeal carcinoma via targeted regulation of ZEB2.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Junzheng Li,Shifen Zhang,Weiping Xu,Dianyu Shi,Mugai Zhuo,Shaoqin Liang,Wenbin Lei,Chun Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZEB2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (AB223688)

Western blot - Anti-ZEB2 antibody (AB223688)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise