Rabbit Polyclonal ZEB2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB2 aa 700-900.
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Publications
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- Cell death & disease 13:4172022ICAM-1 promotes cancer progression by regulating SRC activity as an adapter protein in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Ji Lim,Jae-Hyeok Kang,Yeon-Ju Kim,Seungmo Kim,Su-Jae LeePubMed 35487888
- Frontiers in pharmacology 12:7279162021Puerarin Prevents Acute Liver Injury Inhibiting Inflammatory Responses and ZEB2 Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunfa Yang,Maomao Wu,Hui Fang,Yue Su,Lingling Zhang,Huan ZhouPubMed 34421621
- Cell cycle (Georgetown, Tex.) 20:1692-17072021ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingbao Guo,Manli Xie,Miao Guo,Feiping Yan,Lihong Li,Rui LiuPubMed 34334113
- International journal of molecular medicine 46:2069-20772020Hesperidin administration suppresses the proliferation of lung cancer cells by promoting apoptosis via targeting the miR‑132/ZEB2 signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Tan et. al.PubMed 33125117
- Molecular medicine reports 20:1672-16822019MicroRNA‑30a regulates cell proliferation, migration, invasion and apoptosis in human nasopharyngeal carcinoma via targeted regulation of ZEB2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Chen et. al.PubMed 31257481
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZEB2 aa 700-900. The exact immunogen used to generate this antibody is proprietary information. Database link O60315
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Associated Products
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Target data
Function
Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed:16061479, PubMed:20516212). Represses transcription of E-cadherin (PubMed:16061479). Represses expression of MEOX2 (PubMed:20516212).
Alternative names
KIAA0569, SIP1, ZFHX1B, ZFX1B, HRIHFB2411, ZEB2, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b, SMADIP1
Recommended products
Rabbit Polyclonal ZEB2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB2 aa 700-900.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZEB2 aa 700-900. The exact immunogen used to generate this antibody is proprietary information. Database link O60315
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZEB2 also known as SIP1 (Smad-interacting protein 1) is a zinc finger E-box-binding homeobox 2 protein. It has a mass of approximately 150 kDa. ZEB2 functions as a transcriptional repressor in cellular processes. This protein can bind to E-box sequences in DNA regulating gene expression by repressing transcription. ZEB2 expresses mainly in neural crest-derived tissues the central nervous system and the developing heart and plays an important role in these areas.
- Biological function summary
ZEB2 influences epithelial-mesenchymal transition (EMT) and acts as a regulator in developmental processes. It is part of a protein complex with Smad proteins which are key transducers of the transforming growth factor-beta (TGF-β) signaling pathway. ZEB2 modulates the expression of genes involved in differentiation proliferation and cell migration which are essential for tissue development and response to environmental cues.
- Pathways
ZEB2 plays roles in the TGF-β and Wnt signaling pathways. It interacts with other proteins such as Smad2/3 in the TGF-β pathway to control gene expression related to EMT and cell differentiation. In the Wnt signaling pathway ZEB2 influences the regulation of genes that mediate cell proliferation and adhesion. These pathways are vital for normal development and homeostasis making ZEB2 an important player in cellular function.
- Associated diseases and disorders
ZEB2 has links to Mowat-Wilson syndrome and cancer progression. In Mowat-Wilson syndrome mutations in the ZEB2 gene lead to neurodevelopmental abnormalities. ZEB2 also contributes to cancer by facilitating EMT which is involved in tumor invasion and metastasis. It connects with other EMT-related proteins such as Snail and Slug in cancer contexts emphasizing its importance in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (ab223688)
Paraffin-embedded human cerebral cortex tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (ab223688)
Paraffin-embedded human glioma tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (ab223688)
Paraffin-embedded human colon tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 antibody (ab223688)
Paraffin-embedded human pancreas tissue stained for Smad Interacting Protein 1 using ab223688 at 1/200 dilution in immunohistochemical analysis. Negative control - no staining.
Western blot - Anti-ZEB2 antibody (ab223688)
All lanes: Western blot - Anti-ZEB2 antibody (ab223688) at 1/100 dilution
All lanes: HEL (human bone marrow erythroleukemia cell line) cell lysate
Predicted band size: 136 kDa
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