AB327146

Anti-ZEB2 Antibody [CL13467]

Name: Anti-ZEB2 Antibody [CL13467] (ab327146) | Abcam
Brand: Abcam Limited
SKU: AB327146
Price: 580 USD
Availability: InStock

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Mouse Monoclonal ZEB2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZEB2.

View Alternative Names

KIAA0569, SIP1, ZFHX1B, ZFX1B, HRIHFB2411, ZEB2, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b, SMADIP1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-ZEB2 Antibody [CL13467] (AB327146)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunofluorescence staining in U-87 cell line using ab327146 at 2–10 µg/ml, showing specific staining of nuclear speckles in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human tonsil using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in germinal center cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human cervix using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in squamous epithelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human cerebral cortex using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using ab327146 at 1 : 20–1 : 50 dilution. Corresponding ZEB2 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human anaplastic oligodendroglioma using ab327146 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in tumor cells.

Supplier Data

Western blot - Anti-ZEB2 Antibody [CL13467] (AB327146)

Western blot analysis in human cell lines SK-MEL-30 and Caco-2 using ab327146 at 1 µg/ml. Corresponding ZEB2 RNA-seq data are presented for the same cell lines. Loading control : Anti-HDAC1 antibody.

All lanes:

Western blot - Anti-ZEB2 Antibody [CL13467] (ab327146)

Lane 2:

Human cell line SK-MEL-30

Lane 3:

Human cell line Caco-2

Predicted band size: 136.45 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2 Antibody [CL13467] (AB327146)

Immunohistochemical staining of human skeletal muscle using ab327146 at 1 : 20–1 : 50 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL13467

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZEB2.

O60315

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed : 16061479, PubMed : 20516212). Represses transcription of E-cadherin (PubMed : 16061479). Represses expression of MEOX2 (PubMed : 20516212).

See full target information ZEB2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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