AB314074

Anti-ZEB2/SIP antibody [BLR115H] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal ZEB2 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus.

View Alternative Names

KIAA0569, SIP1, ZFHX1B, ZFX1B, HRIHFB2411, ZEB2, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b, SMADIP1

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Supplier Data

Immunocytochemistry - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Detection of human ZEB2/SIP in FFPE MDA-MB-435 cells by immunocytochemistry. Antibody : Rabbit anti-ZEB2/SIP recombinant monoclonal antibody [BLR115H] (A700-115). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Detection of human ZEB2/SIP in FFPE prostate carcinoma by immunohistochemistry. Antibody : Rabbit anti-ZEB2/SIP recombinant monoclonal antibody [BLR115H] (A700-115). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MDA-MB-435 cells prepared using NETN lysis buffer. Antibodies : Rabbit antiZEB2 recombinant monoclonal antibody [BLR115H] used for IP at 20 µl/mg lysate. ZEB2 was also immunoprecipitated by rabbit anti-ZEB2 antibodies. For blotting immunoprecipitated ZEB2, A700-115 was used at 1 : 1000. Chemiluminescence with an exposure time of 3 minutes.

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Supplier Data

Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Samples : Whole cell lysate (50 µg) from Hep-G2, MDA-MB-435, HeLa, MOLT-4, HEK293T, RKO, UACC-257, and Jurkat cells prepared using NETN lysis buffer. Antibody : Rabbit antiZEB2 recombinant monoclonal antibody [BLR115H] used at 1 : 1000. Secondary : HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-COPB2 (A300-523A).

All lanes:

Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074) at 1/1000 dilution

Lane 1:

Hep-G2 Whole cell lysate at 50 µg

Lane 2:

MDA-MB-435 Whole cell lysate at 50 µg

Lane 3:

HeLa Whole cell lysate at 50 µg

Lane 4:

MOLT-4 Whole cell lysate at 50 µg

Lane 5:

HEK293T Whole cell lysate at 50 µg

Lane 6:

RKO Whole cell lysate at 50 µg

Lane 7:

UACC-257 Whole cell lysate at 50 µg

Lane 8:

Jurkat Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR115H

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60315

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZEB2 also known as SIP1 (Smad-interacting protein 1) is a zinc finger E-box-binding homeobox 2 protein. It has a mass of approximately 150 kDa. ZEB2 functions as a transcriptional repressor in cellular processes. This protein can bind to E-box sequences in DNA regulating gene expression by repressing transcription. ZEB2 expresses mainly in neural crest-derived tissues the central nervous system and the developing heart and plays an important role in these areas.

Biological function summary

ZEB2 influences epithelial-mesenchymal transition (EMT) and acts as a regulator in developmental processes. It is part of a protein complex with Smad proteins which are key transducers of the transforming growth factor-beta (TGF-β) signaling pathway. ZEB2 modulates the expression of genes involved in differentiation proliferation and cell migration which are essential for tissue development and response to environmental cues.

Pathways

ZEB2 plays roles in the TGF-β and Wnt signaling pathways. It interacts with other proteins such as Smad2/3 in the TGF-β pathway to control gene expression related to EMT and cell differentiation. In the Wnt signaling pathway ZEB2 influences the regulation of genes that mediate cell proliferation and adhesion. These pathways are vital for normal development and homeostasis making ZEB2 an important player in cellular function.

ZEB2 has links to Mowat-Wilson syndrome and cancer progression. In Mowat-Wilson syndrome mutations in the ZEB2 gene lead to neurodevelopmental abnormalities. ZEB2 also contributes to cancer by facilitating EMT which is involved in tumor invasion and metastasis. It connects with other EMT-related proteins such as Snail and Slug in cancer contexts emphasizing its importance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed : 16061479, PubMed : 20516212). Represses transcription of E-cadherin (PubMed : 16061479). Represses expression of MEOX2 (PubMed : 20516212).

See full target information ZEB2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZEB2/SIP antibody [BLR115H] - BSA free

Immunocytochemistry - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Immunoprecipitation - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise