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Rabbit Recombinant Monoclonal ZEB2 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus.

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Images

Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074), expandable thumbnail
  • Immunoprecipitation - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074), expandable thumbnail
  • Immunocytochemistry - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (AB314074), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60315

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIHC-PIPWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed:16061479, PubMed:20516212). Represses transcription of E-cadherin (PubMed:16061479). Represses expression of MEOX2 (PubMed:20516212).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ZEB2 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60315
Clone number

BLR115H

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZEB2 also known as SIP1 (Smad-interacting protein 1) is a zinc finger E-box-binding homeobox 2 protein. It has a mass of approximately 150 kDa. ZEB2 functions as a transcriptional repressor in cellular processes. This protein can bind to E-box sequences in DNA regulating gene expression by repressing transcription. ZEB2 expresses mainly in neural crest-derived tissues the central nervous system and the developing heart and plays an important role in these areas.

Biological function summary

ZEB2 influences epithelial-mesenchymal transition (EMT) and acts as a regulator in developmental processes. It is part of a protein complex with Smad proteins which are key transducers of the transforming growth factor-beta (TGF-β) signaling pathway. ZEB2 modulates the expression of genes involved in differentiation proliferation and cell migration which are essential for tissue development and response to environmental cues.

Pathways

ZEB2 plays roles in the TGF-β and Wnt signaling pathways. It interacts with other proteins such as Smad2/3 in the TGF-β pathway to control gene expression related to EMT and cell differentiation. In the Wnt signaling pathway ZEB2 influences the regulation of genes that mediate cell proliferation and adhesion. These pathways are vital for normal development and homeostasis making ZEB2 an important player in cellular function.

Associated diseases and disorders

ZEB2 has links to Mowat-Wilson syndrome and cancer progression. In Mowat-Wilson syndrome mutations in the ZEB2 gene lead to neurodevelopmental abnormalities. ZEB2 also contributes to cancer by facilitating EMT which is involved in tumor invasion and metastasis. It connects with other EMT-related proteins such as Snail and Slug in cancer contexts emphasizing its importance in disease mechanisms.

Product promise

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4 product images

  • Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074), expandable thumbnail

    Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074)

    Samples: Whole cell lysate (50 µg) from Hep-G2, MDA-MB-435, HeLa, MOLT-4, HEK293T, RKO, UACC-257, and Jurkat cells prepared using NETN lysis buffer. Antibody:Rabbit antiZEB2 recombinant monoclonal antibody [BLR115H] used at 1:1000. Secondary:HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 (A300-523A).

    All lanes: Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074) at 1/1000 dilution

    Lane 1: Hep-G2 Whole cell lysate at 50 µg

    Lane 2: MDA-MB-435 Whole cell lysate at 50 µg

    Lane 3: HeLa Whole cell lysate at 50 µg

    Lane 4: MOLT-4 Whole cell lysate at 50 µg

    Lane 5: HEK293T Whole cell lysate at 50 µg

    Lane 6: RKO Whole cell lysate at 50 µg

    Lane 7: UACC-257 Whole cell lysate at 50 µg

    Lane 8: Jurkat Whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074), expandable thumbnail

    Immunoprecipitation - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074)

    Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MDA-MB-435 cells prepared using NETN lysis buffer. Antibodies: Rabbit antiZEB2 recombinant monoclonal antibody [BLR115H] used for IP at 20 µl/mg lysate. ZEB2 was also immunoprecipitated by rabbit anti-ZEB2 antibodies. For blotting immunoprecipitated ZEB2, A700-115 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.

  • Immunocytochemistry - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074), expandable thumbnail

    Immunocytochemistry - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074)

    Detection of human ZEB2/SIP in FFPE MDA-MB-435 cells by immunocytochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody [BLR115H] (A700-115). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074)

    Detection of human ZEB2/SIP in FFPE prostate carcinoma by immunohistochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody [BLR115H] (A700-115). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

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Product protocols

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