Rabbit Recombinant Monoclonal ZEB2 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
ICC | IHC-P | IP | WB | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters (PubMed:16061479, PubMed:20516212). Represses transcription of E-cadherin (PubMed:16061479). Represses expression of MEOX2 (PubMed:20516212).
KIAA0569, SIP1, ZFHX1B, ZFX1B, HRIHFB2411, ZEB2, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b, SMADIP1
Rabbit Recombinant Monoclonal ZEB2 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZEB2 aa 1150 to C-terminus.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
BLR115H
Blue Ice
Up to 12 months
+4°C
+4°C
This supplementary information is collated from multiple sources and compiled automatically.
ZEB2 also known as SIP1 (Smad-interacting protein 1) is a zinc finger E-box-binding homeobox 2 protein. It has a mass of approximately 150 kDa. ZEB2 functions as a transcriptional repressor in cellular processes. This protein can bind to E-box sequences in DNA regulating gene expression by repressing transcription. ZEB2 expresses mainly in neural crest-derived tissues the central nervous system and the developing heart and plays an important role in these areas.
ZEB2 influences epithelial-mesenchymal transition (EMT) and acts as a regulator in developmental processes. It is part of a protein complex with Smad proteins which are key transducers of the transforming growth factor-beta (TGF-β) signaling pathway. ZEB2 modulates the expression of genes involved in differentiation proliferation and cell migration which are essential for tissue development and response to environmental cues.
ZEB2 plays roles in the TGF-β and Wnt signaling pathways. It interacts with other proteins such as Smad2/3 in the TGF-β pathway to control gene expression related to EMT and cell differentiation. In the Wnt signaling pathway ZEB2 influences the regulation of genes that mediate cell proliferation and adhesion. These pathways are vital for normal development and homeostasis making ZEB2 an important player in cellular function.
ZEB2 has links to Mowat-Wilson syndrome and cancer progression. In Mowat-Wilson syndrome mutations in the ZEB2 gene lead to neurodevelopmental abnormalities. ZEB2 also contributes to cancer by facilitating EMT which is involved in tumor invasion and metastasis. It connects with other EMT-related proteins such as Snail and Slug in cancer contexts emphasizing its importance in disease mechanisms.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Samples: Whole cell lysate (50 µg) from Hep-G2, MDA-MB-435, HeLa, MOLT-4, HEK293T, RKO, UACC-257, and Jurkat cells prepared using NETN lysis buffer. Antibody:Rabbit antiZEB2 recombinant monoclonal antibody [BLR115H] used at 1:1000. Secondary:HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 (A300-523A).
All lanes: Western blot - Anti-ZEB2/SIP antibody [BLR115H] - BSA free (ab314074) at 1/1000 dilution
Lane 1: Hep-G2 Whole cell lysate at 50 µg
Lane 2: MDA-MB-435 Whole cell lysate at 50 µg
Lane 3: HeLa Whole cell lysate at 50 µg
Lane 4: MOLT-4 Whole cell lysate at 50 µg
Lane 5: HEK293T Whole cell lysate at 50 µg
Lane 6: RKO Whole cell lysate at 50 µg
Lane 7: UACC-257 Whole cell lysate at 50 µg
Lane 8: Jurkat Whole cell lysate at 50 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MDA-MB-435 cells prepared using NETN lysis buffer. Antibodies: Rabbit antiZEB2 recombinant monoclonal antibody [BLR115H] used for IP at 20 µl/mg lysate. ZEB2 was also immunoprecipitated by rabbit anti-ZEB2 antibodies. For blotting immunoprecipitated ZEB2, A700-115 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.
Detection of human ZEB2/SIP in FFPE MDA-MB-435 cells by immunocytochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody [BLR115H] (A700-115). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human ZEB2/SIP in FFPE prostate carcinoma by immunohistochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody [BLR115H] (A700-115). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com