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AB73643

Anti-Zebrafish Gut Absorptive Cell Epitopes antibody [FIS 4E8/1]

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(5 Publications)

Mouse Monoclonal Zebrafish Gut Absorptive Cell Epitopes antibody. Suitable for IP, WB, ICC/IF, IHC - Wmt and reacts with Zebrafish samples. Cited in 5 publications. Immunogen corresponding to Tissue preparation containing Zebrafish Gut Absorptive Cell Epitopes protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FIS 4E8/1

Isotype

IgG1

Carrier free

No

Reacts with

Zebrafish

Applications

IHC - Wmt, IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "<p></p>" } } }

Product details

The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyx at the apical surface of intestinal absorptive cells. This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 219: PubMed32328632

2020

Distinct roles for luminal acidification in apical protein sorting and trafficking in zebrafish.

Applications

IP

Species

Zebrafish

Daniel S Levic,Sean Ryan,Lindsay Marjoram,Jamie Honeycutt,Jennifer Bagwell,Michel Bagnat

Developmental cell 51:7-20.e6 PubMed31474562

2019

Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut.

Applications

Unspecified application

Species

Unspecified reactive species

Jieun Park,Daniel S Levic,Kaelyn D Sumigray,Jennifer Bagwell,Oznur Eroglu,Carina L Block,Cagla Eroglu,Robert Barry,Colin R Lickwar,John F Rawls,Stephen A Watts,Terry Lechler,Michel Bagnat

PLoS pathogens 15:e1007381 PubMed30845179

2019

Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin C Murdoch,Scott T Espenschied,Molly A Matty,Olaf Mueller,David M Tobin,John F Rawls

Development (Cambridge, England) 141:1110-9 PubMed24504339

2014

Single continuous lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling.

Applications

ICC/IF

Species

Zebrafish

Ashley L Alvers,Sean Ryan,Paul J Scherz,Jan Huisken,Michel Bagnat

Development (Cambridge, England) 132:1093-104 PubMed15689380

2005

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Cécile Crosnier,Neil Vargesson,Stephen Gschmeissner,Linda Ariza-McNaughton,Alastair Morrison,Julian Lewis
View all publications

Product promise

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