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AB71286

Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2]

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(39 Publications)

Mouse Monoclonal Zebrafish Gut Secretory Cell Epitopes antibody. Suitable for IHC-P and reacts with Zebrafish samples. Cited in 39 publications. Immunogen corresponding to Tissue preparation containing Zebrafish Gut Secretory Cell Epitopes protein.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (AB71286)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] (AB71286)

IHC image of Zebrafish Gut Secretory Cell Epitopes staining in a section of formalin-fixed paraffin-embedded Zebra fish Larvae (5 days) performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab71286, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FIS 2F11/2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Zebrafish

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5260 PubMed40480975

2025

Cholangiocytes contribute to hepatocyte regeneration after partial liver injury during growth spurt in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Sema Elif Eski,Jiarui Mi,Macarena Pozo-Morales,Gabriel Garnik Hovhannisyan,Camille Perazzolo,Rita Manco,Imane Ez-Zammoury,Dev Barbhaya,Anne Lefort,Frédérick Libert,Federico Marini,Esteban N Gurzov,Olov Andersson,Sumeet Pal Singh

Development (Cambridge, England) 152: PubMed39963927

2025

Khdrbs1 drives re-differentiation of bipotential progenitor cells by inhibiting p53 in zebrafish biliary-mediated liver regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Gang,Qi Chen,Junhui Sun,Tingwei Zhang,Pengcheng Cai,Rui Ni,Jianlong Ma

Cell reports 43:114941 PubMed39636728

2024

Myosin 1b regulates intestinal epithelial morphogenesis via interaction with UNC45A.

Applications

Unspecified application

Species

Unspecified reactive species

Céline Revenu,Corinne Lebreton,Magda Cannata Serio,Marion Rosello,Rémi Duclaux-Loras,Karine Duroure,Ophélie Nicolle,Fanny Eggeler,Marie-Thérèse Prospéri,Julie Stoufflet,Juliette Vougny,Priscilla Lépine,Grégoire Michaux,Nadine Cerf-Bensussan,Evelyne Coudrier,Franck Perez,Marianna Parlato,Filippo Del Bene

Development (Cambridge, England) 151: PubMed38975841

2024

Development of a hepatic cryoinjury model to study liver regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Marcos Sande-Melon,David Bergemann,Miriam Fernández-Lajarín,Juan Manuel González-Rosa,Andrew G Cox

Disease models & mechanisms 16: PubMed37675454

2023

Loss of zebrafish pkd1l1 causes biliary defects that have implications for biliary atresia splenic malformation.

Applications

Unspecified application

Species

Unspecified reactive species

Rouknuddin Q Ali,Anne Meyer-Miner,Marie David-Rachel,Fiona J H Lee,Benjamin J Wilkins,Saul J Karpen,Brian Ciruna,Anand Ghanekar,Binita M Kamath

The Journal of clinical investigation 133: PubMed37490339

2023

Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Karolczak,Ashish R Deshwar,Evangelina Aristegui,Binita M Kamath,Michael W Lawlor,Gaia Andreoletti,Jonathan Volpatti,Jillian L Ellis,Chunyue Yin,James J Dowling

NPJ Regenerative medicine 8:20 PubMed37024481

2023

The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingmei Song,Jianlong Ma,Xing Liu,Zhuofu Huang,Lianghui Li,Linke Li,Lingfei Luo,Rui Ni,Jianbo He

Journal of applied toxicology : JAT 43:1073-1082 PubMed36755374

2023

Roxadustat induces hepatotoxicity in zebrafish embryos via inhibiting Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dou Yang,Juhua Xiao,Mengqi Wan,Jieping Liu,Ling Huang,Xue Li,Li Zhang,Fasheng Liu,Desheng Liang,Yongliang Zheng,Baogang Xie,Xinjun Liao,Guanghua Xiong,Huiqiang Lu,Zigang Cao,Shouhua Zhang

Proceedings of the National Academy of Sciences of the United States of America 119:e2201097119 PubMed36469766

2022

Regenerative failure of intrahepatic biliary cells in Alagille syndrome rescued by elevated Jagged/Notch/Sox9 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chengjian Zhao,Jonathan Matalonga,Joseph J Lancman,Lu Liu,Chaoxin Xiao,Shiv Kumar,Keith P Gates,Jiaye He,Alyssa Graves,Jan Huisken,Mizuki Azuma,Zhenghao Lu,Chong Chen,Bi-Sen Ding,P Duc Si Dong

Cells 11: PubMed36291055

2022

Shikonin Inhibits Fin Regeneration in Zebrafish Larvae.

Applications

Unspecified application

Species

Unspecified reactive species

Zigang Cao,Chen Guo,Guilan Chen,Jiejun Liu,Huiwen Ni,Fangsheng Liu,Guanghua Xiong,Xinjun Liao,Huiqiang Lu
View all publications

Product promise

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