Rabbit Polyclonal ZFAND5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFAND5 aa 50-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZFAND5 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link O76080
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Involved in protein degradation via the ubiquitin-proteasome system. May act by anchoring ubiquitinated proteins to the proteasome. Plays a role in ubiquitin-mediated protein degradation during muscle atrophy. Plays a role in the regulation of NF-kappa-B activation and apoptosis. Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Is a potent inhibitory factor for osteoclast differentiation.
Alternative names
ZA20D2, ZNF216, ZFAND5, AN1-type zinc finger protein 5, Zinc finger A20 domain-containing protein 2, Zinc finger protein 216
Recommended products
Rabbit Polyclonal ZFAND5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFAND5 aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZFAND5 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link O76080
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZFAND5 also known as ZNF216 is a protein involved mechanically in protein degradation processes. It has a molecular mass of approximately 24 kDa. This protein is known for its expression in various tissues including muscle and liver. ZFAND5 functions as an adaptor that facilitates the recruitment of proteins to proteasomes aiding in the removal of misfolded or damaged proteins within cells.
- Biological function summary
ZFAND5 has a significant role in maintaining cellular protein homeostasis. The protein is part of a complex involving the ubiquitin-proteasome system. This complex acts to recognize proteins marked for degradation. By interacting with the proteasome ZFAND5 enhances the degradation process which is essential for cellular health and function. Its activity ensures the smooth turnover of cellular proteins preventing accumulation that can lead to cellular stress.
- Pathways
ZFAND5 participates in protein degradation pathways including the ubiquitin-proteasome pathway and the autophagy-lysosome pathway. In the ubiquitin-proteasome pathway ZFAND5 works alongside ubiquitin tagging proteins for destruction. The interplay with other key proteins like ubiquitin ligase highlights its importance in ensuring proteins are correctly degraded and recycled maintaining cellular balance and function.
- Associated diseases and disorders
Aberrant function or expression of ZFAND5 relates to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's the failing protein degradation can lead to harmful accumulation of proteins like amyloid-beta in which ZFAND5's role becomes critical. Additionally the protein associates with chronic inflammatory disorders where proper protein turnover is disrupted connecting with inflammatory mediators through faulty pathways. Understanding ZFAND5's activity offers insights into tackling these conditions effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ZFAND5 antibody (ab272580)
Lane 1: Western blot - Anti-ZFAND5 antibody (ab272580)
Lanes 2 - 3: Western blot - Anti-ZFAND5 antibody (ab272580) at 0.4 µg/mL
Lane 1: Molecular weight ladder
Lane 2: Control (vector only transfected HEK-293T lysate)
Lane 3: ZFAND5 over-expression lysate (mammalian HEK-293T cells)
Predicted band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFAND5 antibody (ab272580)
Paraffin-embedded formalin-fixed Human placenta tissue stained for ZFAND5 using ab272580 at 1/500 in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-ZFAND5 antibody (ab272580)
PFA fixed, Triton X-100 permeablized human cell line A431 labeling ZFAND5 (green) using ab272580 at 2 ug/ml in ICC/IF analysis.
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