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AB272580

Anti-ZFAND5 antibody

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Rabbit Polyclonal ZFAND5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFAND5 aa 50-200.

View Alternative Names

ZA20D2, ZNF216, ZFAND5, AN1-type zinc finger protein 5, Zinc finger A20 domain-containing protein 2, Zinc finger protein 216

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFAND5 antibody (AB272580)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFAND5 antibody (AB272580)

Paraffin-embedded formalin-fixed Human placenta tissue stained for ZFAND5 using ab272580 at 1/500 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ZFAND5 antibody (AB272580)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZFAND5 antibody (AB272580)

PFA fixed, Triton X-100 permeablized human cell line A431 labeling ZFAND5 (green) using ab272580 at 2 ug/ml in ICC/IF analysis.

Western blot - Anti-ZFAND5 antibody (AB272580)
  • WB

Unknown

Western blot - Anti-ZFAND5 antibody (AB272580)

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Western blot - Anti-ZFAND5 antibody (ab272580)

Lanes 2 - 3:

Western blot - Anti-ZFAND5 antibody (ab272580) at 0.4 µg/mL

Lane 1:

Molecular weight ladder

Lane 2:

Control (vector only transfected HEK-293T lysate)

Lane 3:

ZFAND5 over-expression lysate (mammalian HEK-293T cells)

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZFAND5 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

O76080

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZFAND5 also known as ZNF216 is a protein involved mechanically in protein degradation processes. It has a molecular mass of approximately 24 kDa. This protein is known for its expression in various tissues including muscle and liver. ZFAND5 functions as an adaptor that facilitates the recruitment of proteins to proteasomes aiding in the removal of misfolded or damaged proteins within cells.
Biological function summary

ZFAND5 has a significant role in maintaining cellular protein homeostasis. The protein is part of a complex involving the ubiquitin-proteasome system. This complex acts to recognize proteins marked for degradation. By interacting with the proteasome ZFAND5 enhances the degradation process which is essential for cellular health and function. Its activity ensures the smooth turnover of cellular proteins preventing accumulation that can lead to cellular stress.

Pathways

ZFAND5 participates in protein degradation pathways including the ubiquitin-proteasome pathway and the autophagy-lysosome pathway. In the ubiquitin-proteasome pathway ZFAND5 works alongside ubiquitin tagging proteins for destruction. The interplay with other key proteins like ubiquitin ligase highlights its importance in ensuring proteins are correctly degraded and recycled maintaining cellular balance and function.

Aberrant function or expression of ZFAND5 relates to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's the failing protein degradation can lead to harmful accumulation of proteins like amyloid-beta in which ZFAND5's role becomes critical. Additionally the protein associates with chronic inflammatory disorders where proper protein turnover is disrupted connecting with inflammatory mediators through faulty pathways. Understanding ZFAND5's activity offers insights into tackling these conditions effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in protein degradation via the ubiquitin-proteasome system. May act by anchoring ubiquitinated proteins to the proteasome. Plays a role in ubiquitin-mediated protein degradation during muscle atrophy. Plays a role in the regulation of NF-kappa-B activation and apoptosis. Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Is a potent inhibitory factor for osteoclast differentiation.
See full target information ZFAND5
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Product promise

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