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Rabbit Polyclonal ZFAT antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFAT aa 800-1100.

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Images

Western blot - Anti-ZFAT antibody (AB154722), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFAT antibody (AB154722), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human ZFAT aa 800-1100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P243

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Alternatively Tris-EDTA buffer (pH8.0) may be used.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Target data

Function

May be involved in transcriptional regulation. Overexpression causes down-regulation of a number of genes involved in the immune response. Some genes are also up-regulated (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal ZFAT antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFAT aa 800-1100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human ZFAT aa 800-1100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P243
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZFAT also known as zinc finger and AT hook domain containing is a transcription factor with a molecular mass of approximately 130 kDa. It has expression in various tissues including the immune system tissues such as spleen and thymus as well as non-immune tissues like the brain and heart. The protein contains zinc finger motifs and AT hook motifs which allow it to bind DNA and regulate gene transcription. The zinc finger domains help ZFAT interact with specific DNA sequences to influence transcriptional activity while the AT hook domains facilitate attachment to the DNA's minor groove.

Biological function summary

ZFAT plays a role in the immune system development and function. It participates in the regulation of gene expression necessary for maintaining homeostasis of the immune cells. ZFAT acts as part of a protein complex involving other transcription factors and co-regulators to influence the expression of genes associated with immune responses. It affects the differentiation and survival of certain immune cell subtypes such as T cells and B cells which are critical for adaptive immunity.

Pathways

The function of ZFAT integrates into important signaling pathways such as the NF-κB and MAPK pathways. These pathways are essential for mediating immune responses and cellular stress responses. ZFAT interacts with proteins like NF-κB to promote immune cell survival and proliferation. In the MAPK pathway ZFAT can influence signaling cascades that determine cell growth and apoptosis showing its multi-functional role in cellular responses.

Associated diseases and disorders

Abnormal ZFAT function links to autoimmune diseases and some cancers. Specifically altered expression of ZFAT associates with autoimmune diseases like systemic lupus erythematosus (SLE) where it may disrupt normal immune tolerance. In some types of cancer such as lymphoma ZFAT may cooperate with other proteins like BCL2 to contribute to unchecked cell growth and survival. Understanding the role of ZFAT in these diseases could lead to potential therapeutic targets for treatment.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Western blot - Anti-ZFAT antibody (ab154722), expandable thumbnail

    Western blot - Anti-ZFAT antibody (ab154722)

    5% SDS PAGE

    All lanes: Western blot - Anti-ZFAT antibody (ab154722) at 1/1000 dilution

    Lane 1: Jurkat whole cell lysate at 30 µg

    Lane 2: Raji whole cell lysate at 30 µg

    Predicted band size: 139 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFAT antibody (ab154722), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFAT antibody (ab154722)

    Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue labeling ZFAT with Anti-beta Crystallin A3 antibody [EPR9586] ab151722 at a 1/500 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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