Rabbit Polyclonal ZFAT antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFAT aa 800-1100.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Tested |
Rat | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
May be involved in transcriptional regulation. Overexpression causes down-regulation of a number of genes involved in the immune response. Some genes are also up-regulated (By similarity).
KIAA1485, ZFAT1, ZNF406, ZFAT, ZFAT1, KIAA1485, ZNF406, Zinc finger protein ZFAT, Zinc finger gene in AITD susceptibility region, Zinc finger protein 406
Rabbit Polyclonal ZFAT antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFAT aa 800-1100.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
ZFAT also known as zinc finger and AT hook domain containing is a transcription factor with a molecular mass of approximately 130 kDa. It has expression in various tissues including the immune system tissues such as spleen and thymus as well as non-immune tissues like the brain and heart. The protein contains zinc finger motifs and AT hook motifs which allow it to bind DNA and regulate gene transcription. The zinc finger domains help ZFAT interact with specific DNA sequences to influence transcriptional activity while the AT hook domains facilitate attachment to the DNA's minor groove.
ZFAT plays a role in the immune system development and function. It participates in the regulation of gene expression necessary for maintaining homeostasis of the immune cells. ZFAT acts as part of a protein complex involving other transcription factors and co-regulators to influence the expression of genes associated with immune responses. It affects the differentiation and survival of certain immune cell subtypes such as T cells and B cells which are critical for adaptive immunity.
The function of ZFAT integrates into important signaling pathways such as the NF-κB and MAPK pathways. These pathways are essential for mediating immune responses and cellular stress responses. ZFAT interacts with proteins like NF-κB to promote immune cell survival and proliferation. In the MAPK pathway ZFAT can influence signaling cascades that determine cell growth and apoptosis showing its multi-functional role in cellular responses.
Abnormal ZFAT function links to autoimmune diseases and some cancers. Specifically altered expression of ZFAT associates with autoimmune diseases like systemic lupus erythematosus (SLE) where it may disrupt normal immune tolerance. In some types of cancer such as lymphoma ZFAT may cooperate with other proteins like BCL2 to contribute to unchecked cell growth and survival. Understanding the role of ZFAT in these diseases could lead to potential therapeutic targets for treatment.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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5% SDS PAGE
All lanes: Western blot - Anti-ZFAT antibody (ab154722) at 1/1000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Predicted band size: 139 kDa
Immunohistochemical analysis of paraffin embedded Human HBL435 xenograft tissue labeling ZFAT with Anti-beta Crystallin A3 antibody [EPR9586] ab151722 at a 1/500 dilution.
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