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AB249647

Anti-ZFP200 antibody [EPR11002] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ZFP200 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ZNFMF, ZNF200, Zinc finger protein 200

1 Images
Western blot - Anti-ZFP200 antibody [EPR11002] - BSA and Azide free (AB249647)
  • WB

Supplier Data

Western blot - Anti-ZFP200 antibody [EPR11002] - BSA and Azide free (AB249647)

This data was developed using ab171743, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZFP200 antibody [EPR11002] (<a href='/en-us/products/primary-antibodies/zfp200-antibody-epr11002-ab171743'>ab171743</a>) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Human small intestine lysate at 10 µg

Lane 3:

NIH:OVCAR-3 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11002

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249647 is the carrier-free version of ab171743.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZFP200 also known as Zinc Finger Protein 200 plays an important role as a transcription factor within cells. The protein has a molecular mass of approximately 50 kDa. ZFP200 is expressed in various tissues with significant levels observed in the liver and kidney. As a transcription factor it binds specific DNA sequences and influences the transcriptional regulation of genes involved in cellular function and development.
Biological function summary

ZFP200 impacts cell growth and differentiation. It acts as part of a larger multi-protein complex where it interacts with other proteins to regulate gene expression. This regulation is essential for maintaining normal cellular processes and contributing to the developmental stages of an organism. ZFP200's involvement in these processes highlights its function as a modulator of gene expression that ensures the proper response to cellular signaling.

Pathways

ZFP200 participates in the Wnt signaling pathway which is important for the regulation of cell fate and movement. It interacts with proteins like Beta-catenin which is an important player in Wnt signaling. Furthermore ZFP200 is involved in the MAPK signaling pathway where it is related to the Ras protein family. These interactions indicate that ZFP200 may serve as a significant regulatory node within signal transduction networks affecting diverse biological outcomes.

Mutations or dysregulation of ZFP200 have associations with certain cancers and developmental disorders. Research suggests a connection between ZFP200 and hepatocellular carcinoma where it might influence tumor growth and proliferation. Furthermore altered expression of ZFP200 may link to congenital anomalies possibly through interactions with other developmental regulators. Understanding the role of ZFP200 may provide insights into the molecular underpinnings of these diseases and guide therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Could have a role in spermatogenesis.
See full target information ZNF200
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental pharmacology & physiology 52:e70032 PubMed40069996

2025

Pancreatitis Independently Induced by Hypertriglyceridaemia Outlines the Immune Profiles of HTGP in Clinic.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxing Liu,Caixia Liang,Wenjing Yan,Yanfeng Zhang,Yi Pan,Yue Yang,Fangfang Zhang,Xiaojian Liu,Liang Jin
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com