AB204922

Anti-ZFP36L1 (phospho S92) antibody [EPR19926]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal ZFP36L1 phospho S92 antibody. Suitable for Dot, WB and reacts with Mouse, Human, Synthetic peptide - Human, Transfected cell lysate - Human samples. Cited in 1 publication.

View Alternative Names

BERG36, BRF1, ERF1, RNF162B, TIS11B, ZFP36L1, mRNA decay activator protein ZFP36L1, Butyrate response factor 1, EGF-response factor 1, TPA-induced sequence 11b, ERF-1, ZFP36-like 1

3 Images

Lab

Western blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (AB204922)

Blocking and diluting buffer and concentration : 3% NFDM/TBST.

Lysates from the ZFP36L1fl/fl CD4 cre KO mouse were kindly provided by Dr Fiamma Salerno, Turner Lab, Babraham Institute.

Lysates used for the WB were isolated by negative selection using biotinylated abs against Ter119, CD11b, CD11c, Gr1, CD19, B220, F4/80 and NK1.1 to leave a population of CD4 and CD8 positive T cells (the purity of the population was >95% as checked by flow).

Mouse anti-vinculin ab130007, used as the loading control.

All lanes:

Western blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922) at 1/2500 dilution

Lane 1:

Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane) at 20 µg

Lane 2:

Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane) at 20 µg

Lane 3:

Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Untreated membrane) at 20 µg

Lane 4:

ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Untreated membrane) at 20 µg

Lane 5:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (Untreated membrane) at 20 µg

Lane 6:

Unstimulated wild-type mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane) at 20 µg

Lane 7:

Wild-type mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane) at 20 µg

Lane 8:

Unstimulated ZFP36L1 knockout mouse CD4+ and CD8+ T cells, whole cell lysate (Phosphatase treated membrane) at 20 µg

Lane 9:

ZFP36L1 knockout mouse CD4+ and CD8+ T cells treated with 10ng/ml Phorbol-12-myristate-13-acetate (PMA) and 1mM Ionomycin for 3 hours, whole cell lysate (Phosphatase treated membrane) at 20 µg

Lane 10:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate, (Phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>); and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/15000 dilution

Predicted band size: 36 kDa

Observed band size: 36-47 kDa

false

Lab

Western blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (AB204922)

Blocking and diluting buffer and concentration : 2% BSA/TBST.

All plasmids were kindly provided by Dr Fiamma Salerno, Turner Lab, Babraham Institute.

The expression profile observed is consistent with what has been described in the literature (PMID : 17030608).

Exposure time : 8 seconds

All lanes:

Western blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (ab204922) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

HEK-293T transfected with human ZFP36L1 expression vector containing a myc-His-tag®, then treated with alkaline phosphatase for 1 hour on the membrane at 10 µg

Lane 4:

HEK-293T transfected with human ZFP36L1 S92A mutant expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 36 kDa

Observed band size: 36-47 kDa

false

Unknown

Dot Blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (AB204922)

Dot blot analysis of ZFP36L1 (phospho S92) labeled with ab204922 at 1/1000 dilution.

Lane 1 : ZFP36L1 (phospho S92) peptide (aa87-97).
Lane 2 : ZFP36L1 non-phospho peptide (aa87-97).
Lane 3 : ZFP36L1 (phospho S125) peptide (aa120-130).
Lane 4 : ZFP36L1 non-phospho peptide (aa120-130).

Blocking and dilution buffer : 5% NFDM/TBST.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Exposure time : 3 minutes.

Carrier free

Anti-ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19926

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZFP36L1 also known as TIS11b or BRF1 is a member of the CCCH-type zinc finger protein family. It plays a role in the regulation of mRNA stability by binding to AU-rich elements in the 3' UTR of target mRNAs. ZFP36L1 has a molecular mass of approximately 44 kDa and its expression is widespread in various tissues including the immune system and embryonic tissues. Through its activity ZFP36L1 can modulate the levels of mRNAs and consequently the proteins they encode influencing cellular processes such as proliferation and differentiation.

Biological function summary

ZFP36L1 acts as a post-transcriptional regulator of gene expression. It destabilizes mRNAs leading to their decay and reduced translation into proteins. While ZFP36L1 often functions as a monomer it can also form complexes with other proteins involved in RNA metabolism. As a result ZFP36L1 plays a critical role in the turnover of pro-inflammatory cytokines and growth factors impacting cellular responses to external stimuli. This regulatory function is essential for maintaining normal cellular homeostasis under various physiological conditions.

Pathways

ZFP36L1 integrates into signaling pathways such as MAPK and NF-kB which are important for inflammation and cellular stress responses. Within these pathways ZFP36L1 interacts with other signaling molecules like MK2 and p38 kinase. These interactions help mediate ZFP36L1's role in mRNA decay influencing the expression of genes involved in the immune response and cell survival. Its participation in these pathways highlights the regulatory landscape in which ZFP36L1 operates affecting diverse cellular functions.

ZFP36L1 has been linked to immune-related conditions such as rheumatoid arthritis and certain types of cancer. In rheumatoid arthritis ZFP36L1 contributes to chronic inflammation due to its impact on cytokine mRNA turnover. It interplays with proteins like TNF-alpha which are important in the inflammatory cascade. In cancer aberrant regulation of ZFP36L1 can affect tumor progression through alterations in cell cycle regulation and apoptosis. Understanding the role of ZFP36L1 in these diseases provides insight into potential therapeutic approaches targeting mRNA stability mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed : 12198173, PubMed : 15467755, PubMed : 15538381, PubMed : 17030608, PubMed : 19179481, PubMed : 20702587, PubMed : 24700863, PubMed : 25014217, PubMed : 25106868, PubMed : 26542173). Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (PubMed : 15687258). Functions by recruiting the CCR4-NOT deadenylase complex and components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes (PubMed : 15687258, PubMed : 18326031, PubMed : 25106868). Induces also the degradation of ARE-containing mRNAs even in absence of poly(A) tail (By similarity). Binds to 3'-UTR ARE of numerous mRNAs (PubMed : 12198173, PubMed : 15467755, PubMed : 15538381, PubMed : 17030608, PubMed : 19179481, PubMed : 20702587, PubMed : 24700863, PubMed : 25014217, PubMed : 25106868, PubMed : 26542173). Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs) (By similarity). Promotes ARE-mediated mRNA decay of mineralocorticoid receptor NR3C2 mRNA in response to hypertonic stress (PubMed : 24700863). Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA (PubMed : 20702587). Positively regulates monocyte/macrophage cell differentiation by promoting ARE-mediated mRNA decay of the cyclin-dependent kinase CDK6 mRNA (PubMed : 26542173). Promotes degradation of ARE-containing pluripotency-associated mRNAs in embryonic stem cells (ESCs), such as NANOG, through a fibroblast growth factor (FGF)-induced MAPK-dependent signaling pathway, and hence attenuates ESC self-renewal and positively regulates mesendoderm differentiation (By similarity). May play a role in mediating pro-apoptotic effects in malignant B-cells by promoting ARE-mediated mRNA decay of BCL2 mRNA (PubMed : 25014217). In association with ZFP36L2 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity-joining (VDJ) recombination and functional immune cell formation (By similarity). Together with ZFP36L2 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA (By similarity). Participates in the delivery of target ARE-mRNAs to processing bodies (PBs) (PubMed : 17369404). In addition to its cytosolic mRNA-decay function, plays a role in the regulation of nuclear mRNA 3'-end processing; modulates mRNA 3'-end maturation efficiency of the DLL4 mRNA through binding with an ARE embedded in a weak noncanonical polyadenylation (poly(A)) signal in endothelial cells (PubMed : 21832157). Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion (PubMed : 15967811). Plays a role in vasculogenesis and endocardial development (By similarity). Plays a role in the regulation of keratinocyte proliferation, differentiation and apoptosis (PubMed : 27182009). Plays a role in myoblast cell differentiation (By similarity).

See full target information ZFP36L1 phospho S92

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of asthma and allergy 15:529-544 PubMed35515816

2022

MALAT1 Induces Food Allergy by Promoting Release of IL-6 from Dendritic Cells and Suppressing the Immunomodulatory Function of Tregs.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Feng,Xiujuan Xiong,Zhuo Chen,Nan Luo,Yongning Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZFP36L1 (phospho S92) antibody [EPR19926]

Western blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (AB204922)

Western blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (AB204922)

Dot Blot - Anti-ZFP36L1 (phospho S92) antibody [EPR19926] (AB204922)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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