Rabbit Polyclonal ZFP57 antibody. Suitable for WB and reacts with Mouse samples. Cited in 17 publications.
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- Epigenetics & chromatin 15:272022The mismatch-repair proteins MSH2 and MSH6 interact with the imprinting control regions through the ZFP57-KAP1 complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBasilia Acurzio,Francesco Cecere,Carlo Giaccari,Ankit Verma,Rosita Russo,Mariangela Valletta,Bruno Hay Mele,Claudia Angelini,Angela Chambery,Andrea RiccioPubMed 35918739
- Nucleic acids research 50:5080-50942022Epigenome editing reveals core DNA methylation for imprinting control in the Dlk1-Dio3 imprinted domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShin Kojima,Naoya Shiochi,Kazuki Sato,Mamiko Yamaura,Toshiaki Ito,Nodoka Yamamura,Naoki Goto,Mika Odamoto,Shin Kobayashi,Tohru Kimura,Yoichi SekitaPubMed 35544282
- iScience 25:1035412022β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucy LeBlanc,Mijeong Kim,Aparna Kambhampati,Albert J Son,Nereida Ramirez,Jonghwan KimPubMed 34977504
- Protein & cell 13:102-1192021Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongling Zhang,Yuanyuan Li,Yongjian Ma,Chongping Lai,Qian Yu,Guangyong Shi,Jinsong LiPubMed 34865203
- Human molecular genetics 30:1509-15202021The number of the CTCF binding sites of the H19/IGF2:IG-DMR correlates with DNA methylation and expression imprinting in a humanized mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Freschi,Rosita Del Prete,Laura Pignata,Francesco Cecere,Francesco Manfrevola,Monica Mattia,Gilda Cobellis,Angela Sparago,Marisa S Bartolomei,Andrea Riccio,Flavia CerratoPubMed 34132339
- Journal of advanced research 33:81-982021Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuacheng Luo,Xiaoyong Li,Geng G Tian,Dali Li,Changliang Hou,Xinbao Ding,Lin Hou,Qifeng Lyu,Yunze Yang,Austin J Cooney,Wenhai Xie,Ji Xiong,Hu Wang,Xiaodong Zhao,Ji WuPubMed 34603780
- Human molecular genetics 30:564-5742021Humanization of a tandem repeat in IG-DMR causes stochastic restoration of paternal imprinting at mouse Dlk1-Dio3 domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Hara,Miho Terao,Atsumi Tsuji-Hosokawa,Yuya Ogawa,Shuji TakadaPubMed 33709141
- Epigenetics & chromatin 11:362018Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHitomi Matsuzaki et. al.PubMed 29958543
- Kidney international 94:326-3452018Gene expression profiles of glomerular endothelial cells support their role in the glomerulopathy of diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Fu,Chengguo Wei,Weijia Zhang,Detlef Schlondorff,Jinshan Wu,Minchao Cai,Wu He,Margaret H Baron,Peter Y Chuang,Zhihong Liu,John Cijiang He,Kyung LeePubMed 29861058
- Epigenetics & chromatin 11:72018Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea Coluccio et. al.PubMed 29482634
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Target data
Function
Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions (ICRs) (PubMed:15070898, PubMed:18854139, PubMed:23059534, PubMed:30602440). Acts together with ZNF445, but ZFP57 plays the predominant role in imprinting maintenance. In contrast, in humans, ZNF445 seems to be the major factor early embryonic imprinting maintenance (PubMed:30602440). Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Binds to a 5'-TGCCGC-3' consensus sequence and recognizes the methylated CpG within this element (PubMed:15070898, PubMed:18854139, PubMed:23059534).
Alternative names
Zinc finger protein 57, Zfp-57, Zfp57
Recommended products
Rabbit Polyclonal ZFP57 antibody. Suitable for WB and reacts with Mouse samples. Cited in 17 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Zfp57 also known as Zinc finger protein 57 plays a significant mechanical role in the regulation of gene expression. It acts as a transcriptional repressor by binding to DNA methylated sites using its zinc finger domains to recognize specific DNA sequences. The molecular mass of Zfp57 is approximately 58 kDa. Zfp57 is generally expressed in early embryonic stem cells and in the developing mouse embryo indicating a role in early development.
- Biological function summary
Zfp57 maintains DNA methylation at imprinted genes essential for genomic imprinting stability. This protein forms a complex with other proteins including TRIM28 (also known as KAP1) which facilitates its function in establishing and maintaining DNA methylation patterns. By interacting with these partners Zfp57 ensures proper transmission of epigenetic marks across generations.
- Pathways
The activity of Zfp57 associates with the epigenetic regulation pathway particularly playing a role in the maintenance of DNA methylation imprints. It directly interacts with methyl-DNA binding proteins such as MeCP2 which are important in interpreting methylation signals to adjust chromatin conformation. The proper function of Zfp57 within these pathways is necessary for maintaining normal genomic architecture.
- Associated diseases and disorders
Alterations in Zfp57 function could lead to problems like transient neonatal diabetes mellitus and related imprinting disorders. These conditions arise when there is improper methylation imprint maintenance due to Zfp57 malfunction. Moreover the protein connects to other factors like NLRP7 influencing the risk of imprinting disorders that affect normal growth and development during infancy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Image courtesy of Dr Nozomi Takahashi, Ferguson-Smith Lab, University of Cambridge.Western blot - Anti-Zfp57 antibody (ab45341)
All lanes: Western blot - Anti-Zfp57 antibody (ab45341) at 1/1000 dilution
Lanes 1 - 2: Wild type mouse embryonic stem cell lysate
Lane 3: Zfp57 knockout mouse embryonic stem cell lysate
SecondaryAll lanes: Goat anti Rabbit HRP at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
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