AB45341

Anti-Zfp57 antibody

(2 Reviews)

(19 Publications)

Rabbit Polyclonal ZFP57 antibody. Suitable for WB and reacts with Mouse samples. Cited in 19 publications.

View Alternative Names

Zinc finger protein 57, Zfp-57, Zfp57

1 Images

Collaborator

Western blot - Anti-Zfp57 antibody (AB45341)

All lanes:

Western blot - Anti-Zfp57 antibody (ab45341) at 1/1000 dilution

Lanes 1 - 2:

Wild type mouse embryonic stem cell lysate

Lane 3:

Zfp57 knockout mouse embryonic stem cell lysate

Secondary

All lanes:

Goat anti Rabbit HRP at 1/3000 dilution

Predicted band size: 52 kDa

true

Image courtesy of Dr Nozomi Takahashi, Ferguson-Smith Lab, University of Cambridge.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Zfp57 also known as Zinc finger protein 57 plays a significant mechanical role in the regulation of gene expression. It acts as a transcriptional repressor by binding to DNA methylated sites using its zinc finger domains to recognize specific DNA sequences. The molecular mass of Zfp57 is approximately 58 kDa. Zfp57 is generally expressed in early embryonic stem cells and in the developing mouse embryo indicating a role in early development.

Biological function summary

Zfp57 maintains DNA methylation at imprinted genes essential for genomic imprinting stability. This protein forms a complex with other proteins including TRIM28 (also known as KAP1) which facilitates its function in establishing and maintaining DNA methylation patterns. By interacting with these partners Zfp57 ensures proper transmission of epigenetic marks across generations.

Pathways

The activity of Zfp57 associates with the epigenetic regulation pathway particularly playing a role in the maintenance of DNA methylation imprints. It directly interacts with methyl-DNA binding proteins such as MeCP2 which are important in interpreting methylation signals to adjust chromatin conformation. The proper function of Zfp57 within these pathways is necessary for maintaining normal genomic architecture.

Alterations in Zfp57 function could lead to problems like transient neonatal diabetes mellitus and related imprinting disorders. These conditions arise when there is improper methylation imprint maintenance due to Zfp57 malfunction. Moreover the protein connects to other factors like NLRP7 influencing the risk of imprinting disorders that affect normal growth and development during infancy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions (ICRs) (PubMed : 15070898, PubMed : 18854139, PubMed : 23059534, PubMed : 30602440). Acts together with ZNF445, but ZFP57 plays the predominant role in imprinting maintenance. In contrast, in humans, ZNF445 seems to be the major factor early embryonic imprinting maintenance (PubMed : 30602440). Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Binds to a 5'-TGCCGC-3' consensus sequence and recognizes the methylated CpG within this element (PubMed : 15070898, PubMed : 18854139, PubMed : 23059534).

See full target information Zinc finger protein 57

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112380 PubMed37061916

2023

Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures.

Applications

Unspecified application

Species

Unspecified reactive species

Jack-Christophe Cossec,Tatiana Traboulsi,Sébastien Sart,Yann Loe-Mie,Manuel Guthmann,Ivo A Hendriks,Ilan Theurillat,Michael L Nielsen,Maria-Elena Torres-Padilla,Charles N Baroud,Anne Dejean

Epigenetics & chromatin 15:27 PubMed35918739

2022

The mismatch-repair proteins MSH2 and MSH6 interact with the imprinting control regions through the ZFP57-KAP1 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Basilia Acurzio,Francesco Cecere,Carlo Giaccari,Ankit Verma,Rosita Russo,Mariangela Valletta,Bruno Hay Mele,Claudia Angelini,Angela Chambery,Andrea Riccio

Nucleic acids research 50:5080-5094 PubMed35544282

2022

Epigenome editing reveals core DNA methylation for imprinting control in the Dlk1-Dio3 imprinted domain.

Applications

Unspecified application

Species

Unspecified reactive species

Shin Kojima,Naoya Shiochi,Kazuki Sato,Mamiko Yamaura,Toshiaki Ito,Nodoka Yamamura,Naoki Goto,Mika Odamoto,Shin Kobayashi,Tohru Kimura,Yoichi Sekita

iScience 25:103541 PubMed34977504

2022

β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy LeBlanc,Mijeong Kim,Aparna Kambhampati,Albert J Son,Nereida Ramirez,Jonghwan Kim

Protein & cell 13:102-119 PubMed34865203

2021

Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongling Zhang,Yuanyuan Li,Yongjian Ma,Chongping Lai,Qian Yu,Guangyong Shi,Jinsong Li

Molecular cell 81:2944-2959.e10 PubMed34166609

2021

Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Lenka Skalska,Victoria Begley,Manuel Beltran,Saulius Lukauskas,Garima Khandelwal,Peter Faull,Amandeep Bhamra,Manuel Tavares,Rachel Wellman,Andrey Tvardovskiy,Benjamin M Foster,Igor Ruiz de Los Mozos,Javier Herrero,Silvia Surinova,Ambrosius P Snijders,Till Bartke,Richard G Jenner

Human molecular genetics 30:1509-1520 PubMed34132339

2021

The number of the CTCF binding sites of the H19/IGF2:IG-DMR correlates with DNA methylation and expression imprinting in a humanized mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Freschi,Rosita Del Prete,Laura Pignata,Francesco Cecere,Francesco Manfrevola,Monica Mattia,Gilda Cobellis,Angela Sparago,Marisa S Bartolomei,Andrea Riccio,Flavia Cerrato

Journal of advanced research 33:81-98 PubMed34603780

2021

Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization.

Applications

Unspecified application

Species

Unspecified reactive species

Huacheng Luo,Xiaoyong Li,Geng G Tian,Dali Li,Changliang Hou,Xinbao Ding,Lin Hou,Qifeng Lyu,Yunze Yang,Austin J Cooney,Wenhai Xie,Ji Xiong,Hu Wang,Xiaodong Zhao,Ji Wu

Human molecular genetics 30:564-574 PubMed33709141

2021

Humanization of a tandem repeat in IG-DMR causes stochastic restoration of paternal imprinting at mouse Dlk1-Dio3 domain.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Hara,Miho Terao,Atsumi Tsuji-Hosokawa,Yuya Ogawa,Shuji Takada

Epigenetics & chromatin 11:36 PubMed29958543

2018

Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Matsuzaki,Eiichi Okamura,Daichi Kuramochi,Aki Ushiki,Katsuhiko Hirakawa,Akiyoshi Fukamizu,Keiji Tanimoto

View all publications

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Product promise

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Anti-Zfp57 antibody

Western blot - Anti-Zfp57 antibody (AB45341)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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