AB117787

Anti-ZFP64 antibody

Name: Anti-ZFP64 antibody (ab117787) | Abcam
Brand: Abcam Limited
SKU: AB117787

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(1 Publication)

Rabbit Polyclonal ZFP64 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZFP64 aa 400-450.

View Alternative Names

ZNF338, ZFP64, Zinc finger protein 64, Zfp-64, Zinc finger protein 338

2 Images

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Immunoprecipitation - Anti-ZFP64 antibody (AB117787)

Detection of ZFP64 by Western Blot of Immunprecipitate.
ab117787 at 1µg/ml staining ZFP64 in 293T whole cell lysate immunoprecipitated using ab117787 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZFP64 antibody (ab117787)

Predicted band size: 74 kDa

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Western blot - Anti-ZFP64 antibody (AB117787)

All lanes:

Western blot - Anti-ZFP64 antibody (ab117787) at 0.04 µg/mL

Lane 1:

293T whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 15 µg

Lane 3:

Jurkat whole cell lysates at 50 µg

Lane 4:

HeLa whole cell lysates at 50 µg

Lane 5:

NIH 3T3 whole cell lysates at 50 µg

Predicted band size: 74 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZFP64 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q9NTW7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab117787 was affinity purified using an epitope specific to ZFP64 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZFP64 also known as Zinc Finger Protein 64 acts as a transcription factor with a molecular mass of approximately 71 kDa. This protein contains multiple zinc finger domains and is highly expressed in various tissues including the brain liver and lymphoid tissues. ZFP64 regulates the transcription of target genes by binding to specific DNA sequences influencing gene expression patterns in cells.

Biological function summary

Zinc Finger Protein 64 is involved in critical cellular processes such as cell differentiation and proliferation. It contributes to gene regulation in the context of chromatin remodeling complexes but not as a direct component. ZFP64 plays significant roles in immune response modulation and neural development affecting both protein coding and non-coding regions of the genome. Research has shown its involvement in T-cell regulation and neuronal lineage determination.

Pathways

Zinc Finger Protein 64 plays an important role in the Wnt signaling pathway and the NF-kB signaling pathway. In the Wnt signaling pathway ZFP64 interacts with other proteins to control cellular processes such as growth and migration. In the NF-kB pathway ZFP64 cooperates with other transcription factors to regulate inflammatory responses and immune system activation interacting with proteins such as IKK and RelA in the process.

Research connects Zinc Finger Protein 64 to the development of autoimmune diseases and certain types of cancer such as leukemia. Alterations in ZFP64 expression or mutations may impact the regulation of immune genes leading to uncontrolled immune responses or tumor progression. The protein is also linked to aberrations in the activity of MYC in cancerous conditions influencing the cell cycle and apoptosis pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in the regulation of mesenchymal cell differentiation through transactivation of NOTCH1 target genes.

See full target information ZFP64

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed37627542

2023

Quercetin's Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuele Fosso,Manuela Leo,Livio Muccillo,Vittorio Maria Mandrone,Maria Chiara Di Meo,Annamaria Molinario,Ettore Varricchio,Lina Sabatino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZFP64 antibody

Immunoprecipitation - Anti-ZFP64 antibody (AB117787)

Western blot - Anti-ZFP64 antibody (AB117787)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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