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AB272572

Anti-ZFR antibody

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Rabbit Polyclonal ZFR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZFR aa 450-600.

View Alternative Names

Zinc finger RNA-binding protein, hZFR, M-phase phosphoprotein homolog, ZFR

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFR antibody (AB272572)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFR antibody (AB272572)

Paraffin-embedded formalin-fixed Human pancreas tissue stained for ZFR using ab272572 at 1/50 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ZFR antibody (AB272572)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZFR antibody (AB272572)

PFA fixed, Triton X-100 permeablized human cell line U-251 MG labeling ZFR (green) using ab272572 at 2 ug/ml in ICC/IF analysis.

Western blot - Anti-ZFR antibody (AB272572)
  • WB

Supplier Data

Western blot - Anti-ZFR antibody (AB272572)

All lanes:

Western blot - Anti-ZFR antibody (ab272572) at 0.04 µg/mL

All lanes:

K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate

Predicted band size: 117 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZFR aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

Q96KR1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger RNA-binding protein commonly known as ZFR functions mechanically as an RNA-binding protein that plays a role in RNA processing and regulation. ZFR with a molecular mass of around 115 kDa contains zinc finger motifs that facilitate its binding to RNA. It is widely expressed across various tissues with significant expression in brain and muscle tissues. ZFR interacts specifically with RNA molecules to influence their stability and processing.
Biological function summary

ZFR regulates several processes related to RNA metabolism including splicing and degradation important for maintaining cellular RNA homeostasis. ZFR also participates as a subunit in larger ribonucleoprotein complexes which are essential for post-transcriptional gene regulation. Its activity in facilitating RNA transport and stability links it to essential cellular processes impacting gene expression at the post-transcriptional level.

Pathways

ZFR engages in regulatory networks that include pathways involved in RNA processing and stabilization. It plays a part in mRNA splicing pathways important for producing mature mRNA molecules from pre-mRNA. This interaction closely relates ZFR to other proteins such as SMN and other splicing factors integrating ZFR into broader gene expression and RNA management networks.

Alterations in ZFR expression or function have links to neurological conditions such as amyotrophic lateral sclerosis (ALS). This link is through the dysregulation of RNA-binding proteins like TDP-43 which ZFR connects with in RNA metabolic pathways affected in ALS. Additionally ZFR-related anomalies also show associations with certain muscular disorders due to its high expression in muscle and its role in RNA processing within these tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in postimplantation and gastrulation stages of development. Involved in the nucleocytoplasmic shuttling of STAU2. Binds to DNA and RNA (By similarity).
See full target information ZFR
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Product promise

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