AB185541

Anti-ZFY antibody [EPR11074]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ZFY antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Zinc finger Y-chromosomal protein, ZFY

3 Images

Flow Cytometry (Intracellular) - Anti-ZFY antibody [EPR11074] (AB185541)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZFY antibody [EPR11074] (AB185541)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed HepG2 cells labeling ZFY with ab185541 at 1/220 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

Supplier Data

Western blot - Anti-ZFY antibody [EPR11074] (AB185541)

All lanes:

Western blot - Anti-ZFY antibody [EPR11074] (ab185541) at 1/1000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 90 kDa

Observed band size: 91 kDa

false

Supplier Data

Western blot - Anti-ZFY antibody [EPR11074] (AB185541)

All lanes:

Western blot - Anti-ZFY antibody [EPR11074] (ab185541) at 1/5000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 90 kDa

false

Carrier free

Anti-ZFY antibody [EPR11074] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11074

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab185541 may cross-react with ZFX based on homology search showing these proteins (ZFX and ZFY) are 96% identical.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/220", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ZFY protein also known as Zinc Finger Protein Y-linked is a transcription factor with a mass of approximately 69 kDa. It originates from the Y chromosome and is expressed mainly in male-specific tissues such as the testes. The protein contains C2H2-type zinc finger domains which facilitate its ability to bind DNA and modulate the transcription of specific genes. Its positioning on the Y chromosome links it directly to male-related biological processes.

Biological function summary

ZFY influences gene expression by binding to specific DNA sequences which impacts cellular development and differentiation. As a transcription factor it often works alone rather than as part of a larger complex. In certain contexts it plays a role in male sex determination and spermatogenesis suggesting its importance in regulating genes important for male germ cell development.

Pathways

ZFY plays a significant role in the sex differentiation pathway and spermatogenesis pathway. It interacts with other Y chromosome-encoded proteins like SRY which is important for initiating male sex determination. Moreover juxtaposing proteins such as SOX9 also engage in the pathways where ZFY exerts influence although ZFY mainly functions independently.

ZFY has connections with infertility in males and certain sex chromosome disorders. Researchers link defects or aberrant expression of ZFY to male infertility due to its critical role in spermatogenesis. Additionally it associates with Turner’s syndrome when aberrations in its expression occur highlighting its interaction with other Y chromosome-related proteins including TSPY.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable transcriptional activator. Binds to the consensus sequence 5'-AGGCCY-3'.

See full target information ZFY

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 14:6243-6255 PubMed36247237

2022

Methionine aminopeptidase 2 as a potential target in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eliana Steinberg,Rawnaq Esa,Ouri Schwob,Tal Stern,Natalie Orehov,Gideon Zamir,Ayala Hubert,Dipak Panigrahy,Ofra Benny

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZFY antibody [EPR11074]

Flow Cytometry (Intracellular) - Anti-ZFY antibody [EPR11074] (AB185541)

Western blot - Anti-ZFY antibody [EPR11074] (AB185541)

Western blot - Anti-ZFY antibody [EPR11074] (AB185541)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise