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AB250832

Anti-ZFY antibody [EPR11074] - BSA and Azide free

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Rabbit Recombinant Monoclonal ZFY antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Zinc finger Y-chromosomal protein, ZFY

3 Images
Flow Cytometry (Intracellular) - Anti-ZFY antibody [EPR11074] - BSA and Azide free (AB250832)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZFY antibody [EPR11074] - BSA and Azide free (AB250832)

This data was developed using ab185541, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed HepG2 cells labeling ZFY with ab185541 at 1/220 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

Western blot - Anti-ZFY antibody [EPR11074] - BSA and Azide free (AB250832)
  • WB

Supplier Data

Western blot - Anti-ZFY antibody [EPR11074] - BSA and Azide free (AB250832)

This data was developed using ab185541, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZFY antibody [EPR11074] (<a href='/en-us/products/primary-antibodies/zfy-antibody-epr11074-ab185541'>ab185541</a>) at 1/1000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 90 kDa

Observed band size: 91 kDa

false

Western blot - Anti-ZFY antibody [EPR11074] - BSA and Azide free (AB250832)
  • WB

Supplier Data

Western blot - Anti-ZFY antibody [EPR11074] - BSA and Azide free (AB250832)

This data was developed using ab185541, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZFY antibody [EPR11074] (<a href='/en-us/products/primary-antibodies/zfy-antibody-epr11074-ab185541'>ab185541</a>) at 1/5000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11074

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody may cross-react with ZFX based on homology search showing these proteins (ZFX and ZFY) are 96% identical.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250832 is the carrier-free version of ab185541.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ZFY protein also known as Zinc Finger Protein Y-linked is a transcription factor with a mass of approximately 69 kDa. It originates from the Y chromosome and is expressed mainly in male-specific tissues such as the testes. The protein contains C2H2-type zinc finger domains which facilitate its ability to bind DNA and modulate the transcription of specific genes. Its positioning on the Y chromosome links it directly to male-related biological processes.
Biological function summary

ZFY influences gene expression by binding to specific DNA sequences which impacts cellular development and differentiation. As a transcription factor it often works alone rather than as part of a larger complex. In certain contexts it plays a role in male sex determination and spermatogenesis suggesting its importance in regulating genes important for male germ cell development.

Pathways

ZFY plays a significant role in the sex differentiation pathway and spermatogenesis pathway. It interacts with other Y chromosome-encoded proteins like SRY which is important for initiating male sex determination. Moreover juxtaposing proteins such as SOX9 also engage in the pathways where ZFY exerts influence although ZFY mainly functions independently.

ZFY has connections with infertility in males and certain sex chromosome disorders. Researchers link defects or aberrant expression of ZFY to male infertility due to its critical role in spermatogenesis. Additionally it associates with Turner’s syndrome when aberrations in its expression occur highlighting its interaction with other Y chromosome-related proteins including TSPY.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable transcriptional activator. Binds to the consensus sequence 5'-AGGCCY-3'.
See full target information ZFY
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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