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AB249180

Anti-ZFYVE19 antibody [EPR9789] - BSA and Azide free

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Rabbit Recombinant Monoclonal ZFYVE19 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ANCHR, MPFYVE, ZFYVE19, Abscission/NoCut checkpoint regulator, MLL partner containing FYVE domain, Zinc finger FYVE domain-containing protein 19

2 Images
Western blot - Anti-ZFYVE19 antibody [EPR9789] - BSA and Azide free (AB249180)
  • WB

Unknown

Western blot - Anti-ZFYVE19 antibody [EPR9789] - BSA and Azide free (AB249180)

This data was developed using ab155085, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZFYVE19 antibody [EPR9789] (<a href='/en-us/products/primary-antibodies/zfyve19-antibody-epr9789-ab155085'>ab155085</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

BxPC3 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

false

Flow Cytometry (Intracellular) - Anti-ZFYVE19 antibody [EPR9789] - BSA and Azide free (AB249180)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ZFYVE19 antibody [EPR9789] - BSA and Azide free (AB249180)

This data was developed using ab155085, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized MCF7 cells labeling ZFYVE19 with ab155085 at 1/10 dilution (red) compared with a Rabbit IgG negative control (green).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9789

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249180 is the carrier-free version of ab155085.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZFYVE19 also known as Zinc Finger FYVE-type Containing 19 or ANKFY1 is a protein characterized by the presence of an FYVE domain and ankyrin repeats. This protein has a molecular mass of approximately 104 kDa. ZFYVE19 is expressed broadly in human tissues with high expression levels in the testis brain and heart. Mechanically it plays a pivotal role in vesicular trafficking where the FYVE domain is often involved in binding phosphatidylinositol 3-phosphate on endosomal membranes assisting in the regulation of endosomal-lysosomal sorting.
Biological function summary

This protein plays a significant role in cellular processes related to membrane trafficking and endocytosis. It often participates in protein complexes related to intracellular transport ensuring proper vesicular movements within the cell. ZFYVE19 is known to associate with various molecules that facilitate communication between different cellular compartments. These interactions are essential for cellular homeostasis and affect diverse processes such as signal transduction and nutrient uptake.

Pathways

ZFYVE19 integrates itself in the endosomal-lysosomal pathway and the phosphoinositide signaling pathway. These pathways are important for maintaining cellular lipid homeostasis and endosome maturation. In its function ZFYVE19 interacts with proteins such as EEA1 a well-known early endosome antigen and RAB5A a small GTPase that regulates endocytic vesicle fusion. These interactions help orchestrate the dynamics of membrane trafficking and signal transduction.

Disruptions in ZFYVE19 function have been associated with neurodegenerative diseases and certain cancers. In the context of neurodegenerative diseases a malfunction in endosomal-lysosomal sorting can lead to the accumulation of cellular debris similar to defects observed in disorders like Alzheimer's disease. ZFYVE19's interplay with clathrin which is critical for endocytosis can influence tumorigenesis with alterations in this process contributing to oncogenic transformations in some forms of cancer. Understanding the roles played by ZFYVE19 in these diseases provides insight into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key regulator of abscission step in cytokinesis : part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.
See full target information ZFYVE19
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