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AB319108

Anti-ZFYVE26 antibody [EPR28782-38] - BSA and Azide free

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Rabbit Recombinant Monoclonal ZFY26 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples.

View Alternative Names

KIAA0321, ZFYVE26, Zinc finger FYVE domain-containing protein 26, FYVE domain-containing centrosomal protein, Spastizin, FYVE-CENT

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZFYVE26 antibody [EPR28782-38] - BSA and Azide free (AB319108)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZFYVE26 antibody [EPR28782-38] - BSA and Azide free (AB319108)

This data was developed using ab319107, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling ZFYVE26 with ab319107 at 1/50 (9.88 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in Hela cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Western blot - Anti-ZFYVE26 antibody [EPR28782-38] - BSA and Azide free (AB319108)
  • WB

Supplier Data

Western blot - Anti-ZFYVE26 antibody [EPR28782-38] - BSA and Azide free (AB319108)

This data was developed using ab319107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24367272).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ZFYVE26 antibody [EPR28782-38] (<a href='/en-us/products/primary-antibodies/zfyve26-antibody-epr28782-38-ab319107'>ab319107</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting ZFYVE26 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 285 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-ZFYVE26 antibody [EPR28782-38] - BSA and Azide free (AB319108)
  • WB

Supplier Data

Western blot - Anti-ZFYVE26 antibody [EPR28782-38] - BSA and Azide free (AB319108)

This data was developed using ab319107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24367272).

The identity of the band beneath 285 kDa in lane 1 is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ZFYVE26 antibody [EPR28782-38] (<a href='/en-us/products/primary-antibodies/zfyve26-antibody-epr28782-38-ab319107'>ab319107</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 285 kDa,36 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28782-38

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Not recommended for use in WB with tissue lysates.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

ab319108 is the carrier-free version of ab319107.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZFYVE26 also known as spastizin is a protein with a mass of approximately 260 kDa. It functions as a zinc finger FYVE-type containing protein which participates in intracellular trafficking and endosomal transport. ZFYVE26 locates in the cytoplasm and its expression appears in many tissues but it is particularly abundant in the nervous system where it suggests an important cellular role. Through its FYVE domain it directly binds to phosphatidylinositol 3-phosphate (PtdIns3P) on endosomal membranes a feature that defines its mechanical operation.
Biological function summary

ZFYVE26 regulates endosome-to-trans-Golgi network trafficking and may also facilitate autophagic processes. It forms part of a protein complex involved in shaping the tubular endosomal networks working alongside other proteins like spatacsin. This association aids in maintaining cellular homeostasis by preventing the accumulation of dysfunctional endosomes. ZFYVE26 also influences the stability and positioning of the cytoskeleton by interacting with specific cytoskeletal components.

Pathways

ZFYVE26 plays roles in autophagy and endocytosis pathways. It associates with proteins such as spatacsin and atlastin-1 in these pathways which together modulate membrane dynamics. In autophagy ZFYVE26 ensures the maturation of autophagosomes while in endocytosis it facilitates the recycling of membrane constituents. These interactions ensure proper intracellular communication and material recycling important for cellular regular function.

Defects in ZFYVE26 cause hereditary spastic paraplegia (HSP) a disorder marked by spasticity and weakness of lower limbs. Mutations disrupt its function affecting endosomal pathway and axonal transport leading to neurodegeneration. It's closely associated with proteins like spatacsin in these pathological processes. Additionally ZFYVE26 has connections to amyotrophic lateral sclerosis (ALS) where defective endosomal systems and protein aggregation contribute to motor neuron degeneration and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatidylinositol 3-phosphate-binding protein required for the abscission step in cytokinesis : recruited to the midbody during cytokinesis and acts as a regulator of abscission. May also be required for efficient homologous recombination DNA double-strand break repair.
See full target information ZFYVE26
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com