Anti-ZFYVE26 antibody [EPR28782-59]

• WB

Supplier Data

Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (AB319051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24367272).

The identity of the bands beneath 285 kDa are unknown.

Exposure time : Lanes 1-4 : 92 seconds; Lanes 5-6 : 180 seconds.

All lanes:

Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051) at 1/1000 dilution

Lane 1:

SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 285 kDa

false

• WB

Supplier Data

Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (AB319051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle, heart (PMID : 24367272).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24367272).

Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 and lanes 3-9 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000

The identity of the bands beneath 285 kDa in lane 1, 3 and lane 9 are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse skeletal muscle tissue at 20 µg

Lane 6:

Mouse heart tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 20 µg

Lane 8:

Rat skeletal muscle tissue at 20 µg

Lane 9:

Rat heart tissue lysate at 20 µg

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 3 - 9:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 285 kDa,36 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (AB319051)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24367272).

The identity of the bands beneath 285 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting ZFYVE26 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 285 kDa,36 kDa

false

Exposure time: 92s