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Rabbit Recombinant Monoclonal ZFY26 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (AB319051), expandable thumbnail
  • Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (AB319051), expandable thumbnail
  • Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (AB319051), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IF
Human
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Phosphatidylinositol 3-phosphate-binding protein required for the abscission step in cytokinesis: recruited to the midbody during cytokinesis and acts as a regulator of abscission. May also be required for efficient homologous recombination DNA double-strand break repair.

Alternative names

KIAA0321, ZFYVE26, Zinc finger FYVE domain-containing protein 26, FYVE domain-containing centrosomal protein, Spastizin, FYVE-CENT

Recommended products

Rabbit Recombinant Monoclonal ZFY26 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28782-59
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ZFYVE26 also known as spastizin is a protein with a mass of approximately 260 kDa. It functions as a zinc finger FYVE-type containing protein which participates in intracellular trafficking and endosomal transport. ZFYVE26 locates in the cytoplasm and its expression appears in many tissues but it is particularly abundant in the nervous system where it suggests an important cellular role. Through its FYVE domain it directly binds to phosphatidylinositol 3-phosphate (PtdIns3P) on endosomal membranes a feature that defines its mechanical operation.

Biological function summary

ZFYVE26 regulates endosome-to-trans-Golgi network trafficking and may also facilitate autophagic processes. It forms part of a protein complex involved in shaping the tubular endosomal networks working alongside other proteins like spatacsin. This association aids in maintaining cellular homeostasis by preventing the accumulation of dysfunctional endosomes. ZFYVE26 also influences the stability and positioning of the cytoskeleton by interacting with specific cytoskeletal components.

Pathways

ZFYVE26 plays roles in autophagy and endocytosis pathways. It associates with proteins such as spatacsin and atlastin-1 in these pathways which together modulate membrane dynamics. In autophagy ZFYVE26 ensures the maturation of autophagosomes while in endocytosis it facilitates the recycling of membrane constituents. These interactions ensure proper intracellular communication and material recycling important for cellular regular function.

Associated diseases and disorders

Defects in ZFYVE26 cause hereditary spastic paraplegia (HSP) a disorder marked by spasticity and weakness of lower limbs. Mutations disrupt its function affecting endosomal pathway and axonal transport leading to neurodegeneration. It's closely associated with proteins like spatacsin in these pathological processes. Additionally ZFYVE26 has connections to amyotrophic lateral sclerosis (ALS) where defective endosomal systems and protein aggregation contribute to motor neuron degeneration and disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051), expandable thumbnail

    Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24367272).

    The identity of the bands beneath 285 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting ZFYVE26 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 285 kDa, 36 kDa

    Exposure time: 92s

  • Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051), expandable thumbnail

    Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24367272).

    The identity of the bands beneath 285 kDa are unknown.

    Exposure time: Lanes 1-4: 92 seconds; Lanes 5-6: 180 seconds.

    All lanes: Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051) at 1/1000 dilution

    Lane 1: SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 6: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 285 kDa

  • Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051), expandable thumbnail

    Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: skeletal muscle, heart (PMID: 24367272).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24367272).

    Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 and lanes 3-9 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000

    The identity of the bands beneath 285 kDa in lane 1, 3 and lane 9 are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ZFYVE26 antibody [EPR28782-59] (ab319051) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Mouse brain tissue lysate at 20 µg

    Lane 5: Mouse skeletal muscle tissue at 20 µg

    Lane 6: Mouse heart tissue lysate at 20 µg

    Lane 7: Rat liver tissue lysate at 20 µg

    Lane 8: Rat skeletal muscle tissue at 20 µg

    Lane 9: Rat heart tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 3 - 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 285 kDa, 36 kDa

    Exposure time: 92s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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