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AB314323

Anti-ZFYVE27 antibody [EPR28129-93]

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Rabbit Recombinant Monoclonal ZFY27 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

SPG33, ZFYVE27, Protrudin, Spastic paraplegia 33 protein, Zinc finger FYVE domain-containing protein 27

4 Images
Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)

ZFYVE27 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab314323 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314323 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate Lane 2 : ab314323 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314323 in SH-SY5Y whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/30 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 50s

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)
  • WB

Supplier Data

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates in lane 1-3 were freshly made and used immediately to minimize protein degradation. The bands beneath the target band (50 kDa) in lane 4 and lane 5 are expected to be degradation products.

All lanes:

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Jurkat whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 50 kDa

false

Exposure time: 48s

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)
  • WB

Supplier Data

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver, kidney, stomach(PMID : 17082457) The bands beneath the target band (50 kDa) are expected to be degradation products. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 40 µg

Lane 2:

Human liver tissue lysate at 40 µg

Lane 3:

Human kidney tissue lysate at 40 µg

Lane 4:

Human stomach tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 50 kDa

false

Exposure time: 48s

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)
  • WB

Supplier Data

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (AB314323)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver, stomach(PMID : 17082457) The bands beneath the target band (50 kDa) are expected to be degradation products. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lanes 2 and 8:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse stomach tissue lysate at 20 µg

Lane 4:

Rat cerebellum tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa

false

Exposure time: 114s

  • Carrier free

    Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28129-93

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZFYVE27 also known as Protrudin functions as an active component in intracellular transport processes. The protein has an approximate molecular weight of 50 kDa. It is widely expressed in various tissues with significant levels found in the brain where it interacts with other proteins to facilitate its role in cellular dynamics. ZFYVE27 contains a FYVE domain which aids in binding to phosphatidylinositol 3-phosphate at endosomal membranes supporting its function in membrane trafficking.
Biological function summary

ZFYVE27 plays a role in neurite extension and cellular trafficking. It is not part of a larger complex but interacts independently with other proteins to regulate vesicle formation and transport along microtubules. ZFYVE27 helps in the maintenance of the endoplasmic reticulum's structure and function. Additionally it interacts with proteins like kinesin which assist in the movement of cargo along cellular structures important for proper cell function and communication.

Pathways

ZFYVE27 contributes significantly to the processes of membrane trafficking and neuronal connectivity. It is associated with the endosomal sorting pathway influencing the movement and recycling of vesicles within cells. In this pathway ZFYVE27 interacts with other proteins such as Rab11 which is essential for guiding vesicles to their intended cellular destinations. Additionally it has a role in the axon growth pathway where it cooperates with kinesin motor proteins to promote the elongation and maintenance of neuronal projections.

ZFYVE27 has a connection with hereditary spastic paraplegia (HSP). Mutations in the ZFYVE27 gene lead to neural structural abnormalities contributing to the formation of this neurological disorder characterized by progressive limb spasticity and weakness. The protein interacts with spastin another protein associated with HSP with implications for axonal regeneration and stability. ZFYVE27 also holds a potential link with certain neurodegenerative conditions given its important role in maintaining neuronal network integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (PubMed : 17082457). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity (By similarity). Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner (PubMed : 19289470). Contributes to both the formation and stabilization of the tubular ER network (PubMed : 24668814). Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections (PubMed : 23969831). Acts as an adapter protein and facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (PubMed : 21976701).
See full target information ZFYVE27
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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