Rabbit Recombinant Monoclonal ZFY27 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IP | Flow Cyt (Intra) | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Expected | Not recommended | Not recommended | Not recommended |
Rat | Tested | Expected | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
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Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (PubMed:17082457). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity (By similarity). Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner (PubMed:19289470). Contributes to both the formation and stabilization of the tubular ER network (PubMed:24668814). Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections (PubMed:23969831). Acts as an adapter protein and facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (PubMed:21976701).
SPG33, ZFYVE27, Protrudin, Spastic paraplegia 33 protein, Zinc finger FYVE domain-containing protein 27
Rabbit Recombinant Monoclonal ZFY27 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR28129-93
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
ZFYVE27 also known as Protrudin functions as an active component in intracellular transport processes. The protein has an approximate molecular weight of 50 kDa. It is widely expressed in various tissues with significant levels found in the brain where it interacts with other proteins to facilitate its role in cellular dynamics. ZFYVE27 contains a FYVE domain which aids in binding to phosphatidylinositol 3-phosphate at endosomal membranes supporting its function in membrane trafficking.
ZFYVE27 plays a role in neurite extension and cellular trafficking. It is not part of a larger complex but interacts independently with other proteins to regulate vesicle formation and transport along microtubules. ZFYVE27 helps in the maintenance of the endoplasmic reticulum's structure and function. Additionally it interacts with proteins like kinesin which assist in the movement of cargo along cellular structures important for proper cell function and communication.
ZFYVE27 contributes significantly to the processes of membrane trafficking and neuronal connectivity. It is associated with the endosomal sorting pathway influencing the movement and recycling of vesicles within cells. In this pathway ZFYVE27 interacts with other proteins such as Rab11 which is essential for guiding vesicles to their intended cellular destinations. Additionally it has a role in the axon growth pathway where it cooperates with kinesin motor proteins to promote the elongation and maintenance of neuronal projections.
ZFYVE27 has a connection with hereditary spastic paraplegia (HSP). Mutations in the ZFYVE27 gene lead to neural structural abnormalities contributing to the formation of this neurological disorder characterized by progressive limb spasticity and weakness. The protein interacts with spastin another protein associated with HSP with implications for axonal regeneration and stability. ZFYVE27 also holds a potential link with certain neurodegenerative conditions given its important role in maintaining neuronal network integrity.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: liver, stomach(PMID: 17082457)
The bands beneath the target band (50 kDa) are expected to be degradation products.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/1000 dilution
Lane 1: Mouse cerebellum tissue lysate at 20 µg
Lanes 2 and 8: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse stomach tissue lysate at 20 µg
Lane 4: Rat cerebellum tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 7: Mouse brain tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50 kDa
Exposure time: 114s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates in lane 1-3 were freshly made and used immediately to minimize protein degradation.
The bands beneath the target band (50 kDa) in lane 4 and lane 5 are expected to be degradation products.
All lanes: Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3: NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg
Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 5: Jurkat whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 50 kDa
Exposure time: 48s
ZFYVE27 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab314323 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314323 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2: ab314323 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314323 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/30 dilution
All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 50s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: liver, kidney, stomach(PMID: 17082457)
The bands beneath the target band (50 kDa) are expected to be degradation products.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (ab314323) at 1/1000 dilution
Lane 1: Human hypothalamus tissue lysate at 40 µg
Lane 2: Human liver tissue lysate at 40 µg
Lane 3: Human kidney tissue lysate at 40 µg
Lane 4: Human stomach tissue lysate at 40 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 50 kDa
Exposure time: 48s
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