Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324)

This data was developed using ab314323, the same antibody clone in a different buffer formulation. ZFYVE27 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab314323 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314323 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate Lane 2 : ab314323 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314323 in SH-SY5Y whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] (<a href='/en-us/products/primary-antibodies/zfyve27-antibody-epr28129-93-ab314323'>ab314323</a>) at 1/30 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 50s

• WB

Supplier Data

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324)

This data was developed using ab314323, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates in lane 1-3 were freshly made and used immediately to minimize protein degradation. The bands beneath the target band (50 kDa) in lane 4 and lane 5 are expected to be degradation products.

All lanes:

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (<a href='/en-us/products/primary-antibodies/zfyve27-antibody-epr28129-93-ab314323'>ab314323</a>) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Jurkat whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 50 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324)

This data was developed using ab314323, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver, kidney, stomach(PMID : 17082457) The bands beneath the target band (50 kDa) are expected to be degradation products. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (<a href='/en-us/products/primary-antibodies/zfyve27-antibody-epr28129-93-ab314323'>ab314323</a>) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 40 µg

Lane 2:

Human liver tissue lysate at 40 µg

Lane 3:

Human kidney tissue lysate at 40 µg

Lane 4:

Human stomach tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 50 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324)

This data was developed using ab314323, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver, stomach(PMID : 17082457) The bands beneath the target band (50 kDa) are expected to be degradation products. In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (<a href='/en-us/products/primary-antibodies/zfyve27-antibody-epr28129-93-ab314323'>ab314323</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lanes 2 and 8:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse stomach tissue lysate at 20 µg

Lane 4:

Rat cerebellum tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa

false

Exposure time: 114s