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Rabbit Recombinant Monoclonal ZFY27 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324), expandable thumbnail
  • Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324), expandable thumbnail
  • Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324), expandable thumbnail
  • Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (AB314324), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (PubMed:17082457). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity (By similarity). Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner (PubMed:19289470). Contributes to both the formation and stabilization of the tubular ER network (PubMed:24668814). Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections (PubMed:23969831). Acts as an adapter protein and facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (PubMed:21976701).

Alternative names

SPG33, ZFYVE27, Protrudin, Spastic paraplegia 33 protein, Zinc finger FYVE domain-containing protein 27

Recommended products

Rabbit Recombinant Monoclonal ZFY27 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28129-93
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314324 is the carrier-free version of Anti-ZFYVE27 antibody [EPR28129-93] ab314323.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ZFYVE27 also known as Protrudin functions as an active component in intracellular transport processes. The protein has an approximate molecular weight of 50 kDa. It is widely expressed in various tissues with significant levels found in the brain where it interacts with other proteins to facilitate its role in cellular dynamics. ZFYVE27 contains a FYVE domain which aids in binding to phosphatidylinositol 3-phosphate at endosomal membranes supporting its function in membrane trafficking.

Biological function summary

ZFYVE27 plays a role in neurite extension and cellular trafficking. It is not part of a larger complex but interacts independently with other proteins to regulate vesicle formation and transport along microtubules. ZFYVE27 helps in the maintenance of the endoplasmic reticulum's structure and function. Additionally it interacts with proteins like kinesin which assist in the movement of cargo along cellular structures important for proper cell function and communication.

Pathways

ZFYVE27 contributes significantly to the processes of membrane trafficking and neuronal connectivity. It is associated with the endosomal sorting pathway influencing the movement and recycling of vesicles within cells. In this pathway ZFYVE27 interacts with other proteins such as Rab11 which is essential for guiding vesicles to their intended cellular destinations. Additionally it has a role in the axon growth pathway where it cooperates with kinesin motor proteins to promote the elongation and maintenance of neuronal projections.

Associated diseases and disorders

ZFYVE27 has a connection with hereditary spastic paraplegia (HSP). Mutations in the ZFYVE27 gene lead to neural structural abnormalities contributing to the formation of this neurological disorder characterized by progressive limb spasticity and weakness. The protein interacts with spastin another protein associated with HSP with implications for axonal regeneration and stability. ZFYVE27 also holds a potential link with certain neurodegenerative conditions given its important role in maintaining neuronal network integrity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324), expandable thumbnail

    Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324)

    This data was developed using Anti-ZFYVE27 antibody [EPR28129-93] ab314323, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: liver, stomach(PMID: 17082457)
    The bands beneath the target band (50 kDa) are expected to be degradation products.
    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (Anti-ZFYVE27 antibody [EPR28129-93] ab314323) at 1/1000 dilution

    Lane 1: Mouse cerebellum tissue lysate at 20 µg

    Lanes 2 and 8: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse stomach tissue lysate at 20 µg

    Lane 4: Rat cerebellum tissue lysate at 20 µg

    Lane 5: Rat liver tissue lysate at 20 µg

    Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 7: Mouse brain tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50 kDa

    Exposure time: 114s

  • Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324), expandable thumbnail

    Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324)

    This data was developed using Anti-ZFYVE27 antibody [EPR28129-93] ab314323, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates in lane 1-3 were freshly made and used immediately to minimize protein degradation.
    The bands beneath the target band (50 kDa) in lane 4 and lane 5 are expected to be degradation products.

    All lanes: Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (Anti-ZFYVE27 antibody [EPR28129-93] ab314323) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 3: NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg

    Lane 4: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: Jurkat whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 50 kDa

    Exposure time: 48s

  • Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324), expandable thumbnail

    Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324)

    This data was developed using Anti-ZFYVE27 antibody [EPR28129-93] ab314323, the same antibody clone in a different buffer formulation.
    ZFYVE27 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with Anti-ZFYVE27 antibody [EPR28129-93] ab314323 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ZFYVE27 antibody [EPR28129-93] ab314323 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 2: Anti-ZFYVE27 antibody [EPR28129-93] ab314323 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ZFYVE27 antibody [EPR28129-93] ab314323 in SH-SY5Y whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-ZFYVE27 antibody [EPR28129-93] (Anti-ZFYVE27 antibody [EPR28129-93] ab314323) at 1/30 dilution

    All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 50s

  • Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324), expandable thumbnail

    Western blot - Anti-ZFYVE27 antibody [EPR28129-93] - BSA and Azide free (ab314324)

    This data was developed using Anti-ZFYVE27 antibody [EPR28129-93] ab314323, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: liver, kidney, stomach(PMID: 17082457)
    The bands beneath the target band (50 kDa) are expected to be degradation products.
    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-ZFYVE27 antibody [EPR28129-93] (Anti-ZFYVE27 antibody [EPR28129-93] ab314323) at 1/1000 dilution

    Lane 1: Human hypothalamus tissue lysate at 40 µg

    Lane 2: Human liver tissue lysate at 40 µg

    Lane 3: Human kidney tissue lysate at 40 µg

    Lane 4: Human stomach tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 50 kDa

    Exposure time: 48s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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