Anti-Zhangfei antibody

• WB

Project

Western blot - Anti-Zhangfei antibody (AB28700)

In the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.

All lanes:

Western blot - Anti-Zhangfei antibody (ab28700) at 1 µg/mL

Lane 1:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 20 µg

Lane 2:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 20 µg

Lane 3:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 20 µg

Lane 4:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 20 µg

Lane 5:

293T Embryonic Kidney cell line (Human) Whole Cell Lysate at 20 µg

Lane 6:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 7:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Lane 8:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 9:

PC-3 whole cell lysate (<a href='/en-us/products/unavailable/pc-3-whole-cell-lysate-ab3954'>ab3954</a>) at 20 µg

Lane 10:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 37 kDa

Observed band size: 50 kDa

false

Exposure time: 2min