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AB96083

Anti-ZHX2 antibody

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(2 Publications)

Rabbit Polyclonal ZHX2 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZHX2 aa 500-800.

View Alternative Names

AFR1, KIAA0854, RAF, ZHX2, Zinc fingers and homeoboxes protein 2, Alpha-fetoprotein regulator 1, Regulator of AFP, Zinc finger and homeodomain protein 2, AFP regulator 1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab96083 at 1/200 dilution. (1) ab96083, (2) Merged with DNA probe

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody (AB96083)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ZHX2 protein at nucleus using ab96083 at a 1/500 dilution (Green). Phalloidin, a cytoskeleton marker, was stained at a 1/200 dilution (Red).
Scale bar = 10 µM

Immunoprecipitation - Anti-ZHX2 antibody (AB96083)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZHX2 antibody (AB96083)

Immunoprecipitation of ZHX2 protein from MCF-7 whole cell extracts using 5 ug of ab96083.
Western blot analysis was performed using ab96083.
Anti-Rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-ZHX2 antibody (ab96083) at 5 µg

Lane 1:

MCF-7 whole cell extracts

Lane 2:

MCF-7 whole cell extracts with Control IgG

Lane 3:

MCF-7 whole cell extracts (+)

Secondary

All lanes:

Anti-Rabbit IgG antibody

Predicted band size: 92 kDa

false

Western blot - Anti-ZHX2 antibody (AB96083)
  • WB

Supplier Data

Western blot - Anti-ZHX2 antibody (AB96083)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZHX2 antibody (ab96083) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human ZHX2 aa 500-800. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6X8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Zinc fingers and homeoboxes protein 2 (ZHX2) is a transcriptional repressor with an approximate molecular mass of 95 kDa. ZHX2 is expressed in various tissues including the liver kidney and spleen and it plays a role in regulating gene expression by binding to specific DNA sequences. Also known as zinc fingers and homeoboxes protein 2 ZHX2 contains two C2H2-type zinc finger domains and four homeobox domains which enable its DNA-binding ability and interaction with other proteins.
Biological function summary

ZHX2 impacts gene regulation by interacting with other transcription factors. It is known to associate with ZHX1 and NF-YA to form a protein complex that modulates the expression of various target genes. Through these interactions ZHX2 influences cell division differentiation and survival. It can act as a transcriptional repressor by binding to promoter regions of genes therefore modulating their expression levels in cellular contexts.

Pathways

ZHX2 influences several biological processes. It is involved in the Wnt signaling pathway where it can influence cellular proliferation and differentiation by regulating the expression of target genes. Additionally ZHX2 interacts with the pathways governed by the transforming growth factor-beta (TGF-β) family which are important for cellular proliferation differentiation and apoptosis. ZHX2 can modulate these pathways by interacting with proteins such as SMAD2/3 and β-catenin impacting their downstream effects.

ZHX2 has been linked to cancer progression and autoimmune diseases. In certain cancers ZHX2 expression levels correlate with tumor progression suggesting it may serve as a potential marker or therapeutic target. ZHX2 influences the expression of oncogenes and tumor suppressor genes in this context. In autoimmune conditions it has been implicated in abnormal immune responses potentially by regulating genes involved in immune cell function. Through these diseases ZHX2 is associated with proteins like p53 in cancer and NF-κB in autoimmune disorders affecting their signaling and regulatory roles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional repressor (PubMed : 12741956). Represses the promoter activity of the CDC25C gene stimulated by NFYA (PubMed : 12741956). May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity).
See full target information ZHX2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Hepatology research : the official journal of the Japan Society of Hepatology : PubMed39207766

2024

Elevated nuclear expression of ZHX1 correlates with poor prognosis in hepatocellular carcinoma (HCC): Comparison of nuclear and cytoplasmic distribution of the ZHX family in HCC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hong Ma,Shinya Maekawa,Shinichi Takano,Tatsuya Yamaguchi,Takeshi Ishida,Shinya Takaoka,Masaru Muraoka,Yasuyuki Komiyama,Hitomi Takada,Yuichiro Suzuki,Mitsuaki Sato,Jianglin Fan,Nobuyuki Enomoto

Gene expression 17:7-17 PubMed27197076

2016

Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic Cyp Gene Expression in the Adult Mouse Liver.

Applications

IHC-Fr

Species

Mouse

Kate T Creasy,Jieyun Jiang,Hui Ren,Martha L Peterson,Brett T Spear
View all publications
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Product promise

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