AB205532

Anti-ZHX2 antibody [EPR17303]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal ZHX2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

AFR1, KIAA0854, RAF, ZHX2, Zinc fingers and homeoboxes protein 2, Alpha-fetoprotein regulator 1, Regulator of AFP, Zinc finger and homeodomain protein 2, AFP regulator 1

7 Images

Flow Cytometry (Intracellular) - Anti-ZHX2 antibody [EPR17303] (AB205532)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ZHX2 antibody [EPR17303] (AB205532)

Intracellular Flow Cytometry analysis ofHeLa cells labelling ZHX2 with ab205532 at 1/80 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody [EPR17303] (AB205532)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody [EPR17303] (AB205532)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ZHX2 with ab205532 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows : -
-ve control 1 : - ab205532 at 1/250 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

Lane 1:

HeLa cytosolic fraction at 10 µg

Lane 2:

HeLa nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

All lanes:

NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ZHX2 antibody [EPR17303] (ab205532) at 1/2000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 92 kDa

Observed band size: 110 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17303

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Zinc fingers and homeoboxes protein 2 (ZHX2) is a transcriptional repressor with an approximate molecular mass of 95 kDa. ZHX2 is expressed in various tissues including the liver kidney and spleen and it plays a role in regulating gene expression by binding to specific DNA sequences. Also known as zinc fingers and homeoboxes protein 2 ZHX2 contains two C2H2-type zinc finger domains and four homeobox domains which enable its DNA-binding ability and interaction with other proteins.

Biological function summary

ZHX2 impacts gene regulation by interacting with other transcription factors. It is known to associate with ZHX1 and NF-YA to form a protein complex that modulates the expression of various target genes. Through these interactions ZHX2 influences cell division differentiation and survival. It can act as a transcriptional repressor by binding to promoter regions of genes therefore modulating their expression levels in cellular contexts.

Pathways

ZHX2 influences several biological processes. It is involved in the Wnt signaling pathway where it can influence cellular proliferation and differentiation by regulating the expression of target genes. Additionally ZHX2 interacts with the pathways governed by the transforming growth factor-beta (TGF-β) family which are important for cellular proliferation differentiation and apoptosis. ZHX2 can modulate these pathways by interacting with proteins such as SMAD2/3 and β-catenin impacting their downstream effects.

ZHX2 has been linked to cancer progression and autoimmune diseases. In certain cancers ZHX2 expression levels correlate with tumor progression suggesting it may serve as a potential marker or therapeutic target. ZHX2 influences the expression of oncogenes and tumor suppressor genes in this context. In autoimmune conditions it has been implicated in abnormal immune responses potentially by regulating genes involved in immune cell function. Through these diseases ZHX2 is associated with proteins like p53 in cancer and NF-κB in autoimmune disorders affecting their signaling and regulatory roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional repressor (PubMed : 12741956). Represses the promoter activity of the CDC25C gene stimulated by NFYA (PubMed : 12741956). May play a role in retinal development where it regulates the composition of bipolar cell populations, by promoting differentiation of bipolar OFF-type cells (By similarity). In the brain, may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex (By similarity).

See full target information ZHX2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1600685 PubMed40589742

2025

ScRNA-seq combined with ATAC-seq analysis to explore the metabolic balance mechanism of CCl4-induced liver inflammatory injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Yisha Zhang,Shoubin Ning

Life science alliance 8: PubMed40523799

2025

Transcription arrest induces formation of RNA granules in mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Katja G Hansen,Autum Baxter-Koenigs,Caroline Am Weiss,Erik McShane,L Stirling Churchman

Nutrition & diabetes 15:6 PubMed39987125

2025

ZHX2 inhibits diabetes-induced liver injury and ferroptosis by epigenetic silence of YTHDF2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Meng,Linghua Li

Nature methods 19:959-968 PubMed35927480

2022

PROBER identifies proteins associated with programmable sequence-specific DNA in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Smarajit Mondal,Muthukumar Ramanathan,Weili Miao,Robin M Meyers,Deepti Rao,Vanessa Lopez-Pajares,Zurab Siprashvili,David L Reynolds,Douglas F Porter,Ian Ferguson,Poornima Neela,Yang Zhao,Lindsey M Meservey,Margaret Guo,Yen-Yu Yang,Lin Li,Yinsheng Wang,Paul A Khavari

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZHX2 antibody [EPR17303]

Flow Cytometry (Intracellular) - Anti-ZHX2 antibody [EPR17303] (AB205532)

Immunocytochemistry/ Immunofluorescence - Anti-ZHX2 antibody [EPR17303] (AB205532)

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Western blot - Anti-ZHX2 antibody [EPR17303] (AB205532)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise