Rabbit Polyclonal ZHX3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ZHX3 aa 900 to C-terminus.
View Alternative Names
KIAA0395, TIX1, ZHX3, Zinc fingers and homeoboxes protein 3, Triple homeobox protein 1, Zinc finger and homeodomain protein 3
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZHX3 antibody (AB99353)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99353 at 1/1000 (0.2µg/ml). Detection : DAB.
- IP
Unknown
Immunoprecipitation - Anti-ZHX3 antibody (AB99353)
Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples : Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99353 used for WB at 1 μg/ml and used for IP at 3 μg/mg lysate. ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds.
All lanes:
Immunoprecipitation - Anti-ZHX3 antibody (ab99353)
Predicted band size: 104 kDa
false
- WB
Unknown
Western blot - Anti-ZHX3 antibody (AB99353)
All lanes:
Western blot - Anti-ZHX3 antibody (ab99353) at 0.04 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 15 µg
Lane 3:
HeLa whole cell lysate at 5 µg
Lane 4:
293T cell lysate at 50 µg
Predicted band size: 104 kDa
true
Exposure time: 30s
Reactivity data
Properties and storage information
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Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ZHX3 acts as a transcriptional repressor that works within multi-protein complexes to modulate gene expression. It interacts with other members of the ZHX family and operates in concert with a protein called NF-YA to affect the transcriptional landscape of a cell. Through these interactions ZHX3 impacts cellular processes such as differentiation growth and apoptosis. This protein's activity is essential for maintaining proper cellular functions and ensuring that cells respond correctly to internal and external cues.
Pathways
ZHX3 participates in vital transcriptional regulatory networks. These networks influence cell cycle control and apoptosis both important to normal cellular proliferation and overall homeostasis. In these pathways ZHX3 often interacts with proteins like ZHX2 and NF-YA forming complexes that facilitate its role as a transcriptional regulator. The function of ZHX3 is critical in these pathways to maintain the balance between cell survival and death.
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Target data
Publications (3)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 26: PubMed40430090
2025
Applications
Unspecified application
Species
Unspecified reactive species
Hepatology research : the official journal of the Japan Society of Hepatology : PubMed39207766
2024
Applications
Unspecified application
Species
Unspecified reactive species
Nature methods 19:959-968 PubMed35927480
2022
Applications
Unspecified application
Species
Unspecified reactive species
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