AB133153

Anti-ZIC3 antibody

(1 Review)

(1 Publication)

Goat Polyclonal ZIC3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZIC3 aa 150-200.

View Alternative Names

ZNF203, ZIC3, Zinc finger protein ZIC 3, Zinc finger protein 203, Zinc finger protein of the cerebellum 3

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ZIC3 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

O60481

Specificity

ab133153 is NOT expected to cross-react with other ZIC proteins.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Zinc Finger Protein of the Cerebellum 3 (ZIC3) is a transcription factor involved in the regulation of gene expression. This protein weighing approximately 52 kDa contains a C2H2-type zinc finger domain that facilitates its binding to DNA. ZIC3 plays a role in the development of various body structures such as the heart and neural system. It has its expression mainly in embryonic tissue and neural crest cells. Additionally it appears in tissues like the prostate indicating its involvement beyond developmental stages.

Biological function summary

ZIC3 is essential for normal morphogenesis and is part of a class of proteins that contribute to the formation of body asymmetry during embryogenesis. By interacting with other proteins it acts as a sequence-specific DNA-binding transcription factor. ZIC3 regulates the activity of genes required for the growth and differentiation of cells in vital developmental processes. Though not a part of a larger protein complex it influences complex cellular interactions during embryonic patterning.

Pathways

ZIC3 functions primarily in the Nodal signaling pathway which plays a role in left-right asymmetry in embryos. Within this pathway ZIC3 works in tandem with proteins like Smad2 and Smad3 which transmit the signal for cell fate determination. ZIC3's influence extends to the Hedgehog signaling pathway impacting the regulation of target gene expression during development and interacting with proteins like GLI transcription factors which mediate the cellular response to Hedgehog signals.

ZIC3 mutations are associated with X-linked heterotaxy a congenital condition affecting the left-right asymmetry of thoracic and abdominal organs. It relates closely to proteins within the Nodal signaling pathway where disruptions can cause improper organ placement. Additionally ZIC3 mutations have implications in various congenital heart defects and interactions with other transcription factors such as PITX2 a downstream target of ZIC3 are significant in these cardiac conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.

See full target information ZIC3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:34704-17 PubMed26430963

2015

The transcription factor c-Fos coordinates with histone lysine-specific demethylase 2A to activate the expression of cyclooxygenase-2.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoli Lu,Yang Yang,Yipeng Du,Lin-Lin Cao,Meiting Li,Changchun Shen,Tianyun Hou,Ying Zhao,Haiying Wang,Dajun Deng,Lina Wang,Qihua He,Wei-Guo Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZIC3 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise