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AB308186

Anti-Zika virus Envelope protein antibody [HL1699]

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Rabbit Recombinant Monoclonal Zika virus envelope protein antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - Zika virus samples. Immunogen corresponding to Synthetic Peptide within Zika virus Genome polyprotein.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Zika virus Envelope protein antibody [HL1699] (AB308186)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus Envelope protein antibody [HL1699] (AB308186)

For immunofluorescence analysis, Zika virus non-infected (left) and infected cells (right) were fixed and permeabilized for detection of Zika virus Envelope protein using ab308186 at 1/100 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

Western blot - Anti-Zika virus Envelope protein antibody [HL1699] (AB308186)
  • WB

Supplier Data

Western blot - Anti-Zika virus Envelope protein antibody [HL1699] (AB308186)

Separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Zika virus Envelope protein antibody [HL1699] (ab308186) at 1/2000 dilution

All lanes:

Zika viral lysate at 0.5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1699

Isotype

IgG

Carrier free

Yes

Reacts with

Zika virus

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Zika virus Genome polyprotein. The exact immunogen used to generate this antibody is proprietary information.

Q32ZE1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Zika virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" }, "Zika virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Zika virus envelope protein also known as the E protein facilitates the virus's entry into host cells. This protein participates in the virus's attachment and fusion processes. It generally consists of three domains and has an approximate molecular mass of 53 kDa. Expression of this envelope protein occurs on the surface of the Zika virus particle after its synthesis in the host cell's endoplasmic reticulum. E proteins allow the Zika virus to interact with cell receptors aiding in the virus's ability to establish an infection.
Biological function summary

The Zika virus envelope protein initiates fusion with the host cell membrane enabling the release of viral RNA into the host cytoplasm. This process is not solitary; it works as part of a complex involving other viral proteins like the membrane protein (M protein). The E protein has a role in immune system evasion by altering its structure and glycan shielding which complicates recognition by host antibodies. These aspects help the virus persist and replicate within its host.

Pathways

The Zika virus envelope protein engages in the viral entry and replication pathways. A critical pathway involves receptor-mediated endocytosis reliant on host cell receptors to internalize the virus. The E protein works alongside the prM protein a precursor of the M protein to facilitate structural rearrangement necessary for the membrane fusion. The protein also impacts signaling pathways that influence the host's cell cycle which provides a favorable environment for viral replication and expression.

Several are associated with the activity of the Zika virus envelope protein. It is most notably linked to Zika virus infections which can lead to microcephaly and other severe birth defects in newborns when contracted during pregnancy. The envelope protein interacts with host immune proteins like the complement system which sometimes leads to an exaggerated immune response complicating recovery and potentially causing autoimmunity. Zika's E protein shares similarities with envelope proteins of other flaviviruses such as Dengue virus which further complicates immune recognition due to cross-reactive antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Zika virus envelope protein
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com