Anti-Zika virus envelope protein E antibody [EPR30864-521]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus envelope protein E antibody [EPR30864-521] (AB325556)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Zika virus envelope protein E expression vector containing a His tag cell pellet (B) HEK-293T transfected with an empty expression vector containing a His tag cell pellet tissue labeling Zika virus envelope protein E with ab325556 at 1/5000 (0.107 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Zika virus envelope protein E expression vector containing a His tag cell pellet. No staining on (B) HEK-293T transfected with an empty expression vector containing a His tag cell pellet.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus envelope protein E antibody [EPR30864-521] (AB325556)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc tag
293T transfected with a Virus Zika virus envelope protein E expression vector containing a myc tag

293T transfected with a Tick-borne encephalitis virus envelope protein E expression vector containing a myc tag

293T cells transfected with a Yellow fever virus envelope protein E expression vector containing a myc tag

293T transfected with a Dengue virus type 4 envelope protein E expression vector containing a myc tag

293T transfected with a Dengue virus type 3 envelope protein E expression vector containing a myc tag

293T transfected with a Virus Japanese encephalitis virus envelope protein E expression vector containing a myc tag

293T transfected with a West Nile virus envelope protein E expression vector containing a myc tag cells labelling Zika virus envelope protein E with ab325556 at 1/1000 (0.534 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a Virus Zika virus envelope protein E expression vector containing a myc tag (shown in green), and no staining in 293T cells tranfected with empty vector, Tick-borne encephalitis virus envelope protein E, Yellow fever virus envelope protein E, Dengue virus type 4 envelope protein E, Dengue virus type 3 envelope protein E, Virus Japanese encephalitis virus envelope protein E or West Nile virus envelope protein E expression vectors containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

A. 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc tag

B. 293T transfected with a Virus Zika virus envelope protein E expression vector containing a myc tag

C. 293T transfected with a Tick-borne encephalitis virus envelope protein E expression vector containing a myc tag

D. 293T cells transfected with a Yellow fever virus envelope protein E expression vector containing a myc tag

E. 293T transfected with a Dengue virus type 4 envelope protein E expression vector containing a myc tag

F. 293T transfected with a Dengue virus type 3 envelope protein E expression vector containing a myc tag

G. 293T transfected with a Virus Japanese encephalitis virus envelope protein E expression vector containing a myc tag

H. 293T transfected with a West Nile virus envelope protein E expression vector containing a myc tag

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus envelope protein E antibody [EPR30864-521] (AB325556)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Zika virus envelope protein E with ab325556 at 1/5000 (0.107 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human spleen.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-Zika virus envelope protein E antibody [EPR30864-521] (AB325556)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed envelope protein E from Tick-borne encephalitis virus, Yellow fever virus, Dengue virus type 4, Dengue virus type 3, Japanese encephalitis virus, West Nile virus.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(15-300KDa);Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Zika virus envelope protein E antibody [EPR30864-521] (ab325556) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Virus Zika virus envelope protein E expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Tick-borne encephalitis virus envelope protein E expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Yellow fever virus envelope protein E expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Dengue virus type 4 envelope protein E expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a Dengue virus type 3 envelope protein E expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a Japanese encephalitis virus envelope protein E expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 8:

293T cells transfected with a West Nile virus envelope protein E expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 51 kDa,15-300 kDa,36 kDa

false

Exposure time: 15s