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Mouse Monoclonal POLG antibody. Suitable for ICC/IF, WB and reacts with Zika virus samples.

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Images

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409), expandable thumbnail
  • Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409), expandable thumbnail
  • Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Zika virus
Tested
Tested

Tested
Tested

Species
Zika virus
Dilution info
1/100.00000 - 1/500.00000
Notes

-

Tested
Tested

Species
Zika virus
Dilution info
1/100.00000 - 1/2500.00000
Notes

-

Target data

Recommended products

Mouse Monoclonal POLG antibody. Suitable for ICC/IF, WB and reacts with Zika virus samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Clone number
GT5212
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Zika Virus NS1 also called non-structural protein 1 plays an important role in the viral life cycle. This glycoprotein has an approximate mass of 46-55 kDa and is highly expressed in cells infected by the Zika virus. NS1 is secreted into the extracellular space and found in sera of infected individuals. It exists in different forms with distinct intracellular and extracellular implications displaying versatility in its function and critical role during infection.

Biological function summary

Zika Virus NS1 plays essential roles in immune system evasion and viral replication. NS1 influences the viral pathogenesis through interactions with host immune responses helping the virus avoid recognition. This protein is part of the viral replication complex and contributes to the integrity of viral RNA. The ability of NS1 to modulate host cellular processes enhances the Zika virus's capacity to proliferate and survive within the host.

Pathways

Zika Virus NS1 is central in both evading immune defenses and assisting viral replication. It modulates innate immune pathways including interferon responses limiting host defenses. Through these pathways NS1 interacts with several proteins such as the Toll-like receptors which are critical for detecting viral infections. Additionally NS1 cooperates with other viral non-structural proteins to sustain efficient replication processes.

Associated diseases and disorders

Zika Virus NS1 is linked to congenital Zika syndrome and Guillain-Barré syndrome. NS1 presence in circulation associates with the virus's ability to traverse the placental barrier leading to severe outcomes like microcephaly in newborns. The interactions between NS1 and host proteins such as complement factors further impact the disease progression and severity complicating immune system responses during infection. Understanding NS1's role enhances our ability to diagnose prevent and treat Zika virus-related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409), expandable thumbnail

    Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409)

    Non-infected (–) and infected (+) C6/36 whole cell lysates (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (ab308409) diluted at 1/1000. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409) at 1/1000 dilution

    Lane 1: Non-infected (–) C6/36 whole cell lysates at 30 µg

    Lane 2: Infected (+) C6/36 whole cell lysates at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-mouse IgG antibody

  • Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409), expandable thumbnail

    Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409)

    Non-transfected (–) and transfected (+) HEK293T whole cell lysis (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (ab308409) diluted at 1/1000. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409) at 1/1000 dilution

    Lane 1: Non-transfected (–) HEK293T whole cell lysis at 30 µg

    Lane 2: Transfected (+) HEK293T whole cell lysis at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-mouse IgG antibody

  • Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409), expandable thumbnail

    Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409)

    Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (ab308409) diluted at 1/2500.

    All lanes: Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409) at 1/2500 dilution

    Lane 1: Uninfected vero cells

    Lane 2: infected JEV vero cell lysates

    Lane 3: infected DENV-2 vero cell lysates

    Lane 4: infected ZIKV vero cell lysates

  • Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409)

    For immunofluorescence analysis, non-infected and infected vero cells were permeabilized for detection of Zika virus NS1 protein using ab308409 at 1/500 dilution (green).

  • Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409)

    For immunofluorescence analysis, Zika virus non-infected and infected cells slides were permeabilized for detection of Zika virus NS1 protein using ab308409 at 1/100 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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