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AB308409

Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free

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Mouse Monoclonal POLG antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Zika virus samples.
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Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)
  • WB

Supplier Data

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)

Non-transfected (–) and transfected (+) HEK293T whole cell lysis (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (ab308409) diluted at 1/1000. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409) at 1/1000 dilution

Lane 1:

Non-transfected (–) HEK293T whole cell lysis at 30 µg

Lane 2:

Transfected (+) HEK293T whole cell lysis at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

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Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)

For immunofluorescence analysis, non-infected and infected vero cells were permeabilized for detection of Zika virus NS1 protein using ab308409 at 1/500 dilution (green).

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)

For immunofluorescence analysis, Zika virus non-infected and infected cells slides were permeabilized for detection of Zika virus NS1 protein using ab308409 at 1/100 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)
  • WB

Supplier Data

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)

Non-infected (–) and infected (+) C6/36 whole cell lysates (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (ab308409) diluted at 1/1000. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409) at 1/1000 dilution

Lane 1:

Non-infected (–) C6/36 whole cell lysates at 30 µg

Lane 2:

Infected (+) C6/36 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

false

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)
  • WB

Supplier Data

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (AB308409)

Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus NS1 protein antibody [GT5212] (ab308409) diluted at 1/2500.

All lanes:

Western blot - Anti-Zika virus NS1 protein antibody [GT5212] - BSA and Azide free (ab308409) at 1/2500 dilution

Lane 1:

Uninfected vero cells

Lane 2:

infected JEV vero cell lysates

Lane 3:

infected DENV-2 vero cell lysates

Lane 4:

infected ZIKV vero cell lysates

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT5212

Isotype

IgG1

Carrier free

Yes

Reacts with

Zika virus

Applications

WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zika virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/2500", "WB-species-notes": "<p></p>" } } }
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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zika Virus NS1 also called non-structural protein 1 plays an important role in the viral life cycle. This glycoprotein has an approximate mass of 46-55 kDa and is highly expressed in cells infected by the Zika virus. NS1 is secreted into the extracellular space and found in sera of infected individuals. It exists in different forms with distinct intracellular and extracellular implications displaying versatility in its function and critical role during infection.
Biological function summary

Zika Virus NS1 plays essential roles in immune system evasion and viral replication. NS1 influences the viral pathogenesis through interactions with host immune responses helping the virus avoid recognition. This protein is part of the viral replication complex and contributes to the integrity of viral RNA. The ability of NS1 to modulate host cellular processes enhances the Zika virus's capacity to proliferate and survive within the host.

Pathways

Zika Virus NS1 is central in both evading immune defenses and assisting viral replication. It modulates innate immune pathways including interferon responses limiting host defenses. Through these pathways NS1 interacts with several proteins such as the Toll-like receptors which are critical for detecting viral infections. Additionally NS1 cooperates with other viral non-structural proteins to sustain efficient replication processes.

Zika Virus NS1 is linked to congenital Zika syndrome and Guillain-Barré syndrome. NS1 presence in circulation associates with the virus's ability to traverse the placental barrier leading to severe outcomes like microcephaly in newborns. The interactions between NS1 and host proteins such as complement factors further impact the disease progression and severity complicating immune system responses during infection. Understanding NS1's role enhances our ability to diagnose prevent and treat Zika virus-related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Zika Virus NS1
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Product promise

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