AB308183

Anti-Zika virus NS4B protein antibody [HL1663]

Recombinant
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Rabbit Recombinant Monoclonal Zika virus NS4B protein antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Zika virus, Transfected cell line - Zika virus samples.

4 Images

Immunohistochemistry paraffin embedded sections - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

Immunohistochemical analysis of paraffin-embedded mock and Zika virus NS4B transfected HEK293T labeling Zika virus NS4B protein with ab308183 at 1/1000 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Supplier Data

Western blot - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody [HL1663] (ab308183) diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Zika virus NS4B protein antibody [HL1663] (ab308183) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK-293T whole cell lysate at 30 µg

Lane 2:

Transfected (+) HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 27 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

For immunofluorescence analysis, Zika virus non-infected (left) and infected cells (right) were fixed and permeabilized for detection of Zika virus NS4B protein using ab308183 at 1/100 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

Supplier Data

Western blot - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody [HL1663] (ab308183) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

All lanes:

Western blot - Anti-Zika virus NS4B protein antibody [HL1663] (ab308183) at 1/1000 dilution

Lane 1:

Non-infected (–) C6/36 whole cell lysate at 5 µg

Lane 2:

Infected (+) C6/36 whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1663

Isotype

IgG

Carrier free

Yes

Reacts with

Zika virus

Applications

WB, ICC/IF, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Zika virus": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Zika virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Zika virus envelope protein also known as the E protein facilitates the virus's entry into host cells. This protein participates in the virus's attachment and fusion processes. It generally consists of three domains and has an approximate molecular mass of 53 kDa. Expression of this envelope protein occurs on the surface of the Zika virus particle after its synthesis in the host cell's endoplasmic reticulum. E proteins allow the Zika virus to interact with cell receptors aiding in the virus's ability to establish an infection.

Biological function summary

The Zika virus envelope protein initiates fusion with the host cell membrane enabling the release of viral RNA into the host cytoplasm. This process is not solitary; it works as part of a complex involving other viral proteins like the membrane protein (M protein). The E protein has a role in immune system evasion by altering its structure and glycan shielding which complicates recognition by host antibodies. These aspects help the virus persist and replicate within its host.

Pathways

The Zika virus envelope protein engages in the viral entry and replication pathways. A critical pathway involves receptor-mediated endocytosis reliant on host cell receptors to internalize the virus. The E protein works alongside the prM protein a precursor of the M protein to facilitate structural rearrangement necessary for the membrane fusion. The protein also impacts signaling pathways that influence the host's cell cycle which provides a favorable environment for viral replication and expression.

Several are associated with the activity of the Zika virus envelope protein. It is most notably linked to Zika virus infections which can lead to microcephaly and other severe birth defects in newborns when contracted during pregnancy. The envelope protein interacts with host immune proteins like the complement system which sometimes leads to an exaggerated immune response complicating recovery and potentially causing autoimmunity. Zika's E protein shares similarities with envelope proteins of other flaviviruses such as Dengue virus which further complicates immune recognition due to cross-reactive antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Zika virus NS4B protein

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zika virus NS4B protein antibody [HL1663]

Immunohistochemistry paraffin embedded sections - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

Western blot - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

Western blot - Anti-Zika virus NS4B protein antibody [HL1663] (AB308183)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise