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AB308399

Anti-Zika virus NS4B protein antibody [HL1664]

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Rabbit Recombinant Monoclonal Zika virus NS4B protein antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - Zika virus, Zika virus, Transfected cell lysate - Zika virus samples.
4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)

Immunohistochemical analysis of mock and Zika virus NS4B transfected HEK293T labeling Zika virus NS4B protein using ab308399 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)
  • WB

Supplier Data

Western blot - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody [HL1664] (ab308399) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Zika virus NS4B protein antibody [HL1664] (ab308399) at 1/5000 dilution

All lanes:

Non-transfected (–) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)

For immunofluorescence analysis, Zika virus non-infected and infected cells slide were permeabilized for detection of Zika virus NS4B protein using ab308399 at 1/100 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)
  • WB

Supplier Data

Western blot - Anti-Zika virus NS4B protein antibody [HL1664] (AB308399)

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody [HL1664] (ab308399) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Zika virus NS4B protein antibody [HL1664] (ab308399) at 1/1000 dilution

Lane 1:

Non-infected (–) C6/36 whole cell extracts at 5 µg

Lane 2:

Infected (+) C6/36 whole cell extracts at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1664

Isotype

IgG

Carrier free

Yes

Reacts with

Zika virus

Applications

ICC/IF, WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - Zika virus": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Zika virus": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Zika virus": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zika virus NS4B sometimes called NS4B nonstructural protein is an integral membrane protein with a mass of approximately 27 kDa. The protein localizes to the endoplasmic reticulum (ER) of infected cells. NS4B functions as an important component in the replication of the viral RNA genome. During viral replication NS4B alters the host cell membrane creating structures that favor viral replication. This protein also plays a role as a suppressor of the host's antiviral immune responses by interfering with the signaling pathways that control cytokines and interferons.
Biological function summary

NS4B interacts closely with other nonstructural proteins to form the viral replication complex a multi-protein assembly essential for efficient RNA synthesis. NS4B is known to modify the lipid composition of the ER membrane facilitating the formation of replication platforms essential for viral RNA synthesis. The protein also modulates cellular stress responses helping the virus to evade host immune surveillance. NS4B factors heavily in viral strategy to manipulate host cell processes ensuring successful viral replication and infection propagation.

Pathways

NS4B influences the interferon signaling pathway and the unfolded protein response. It suppresses interferon production impacting the interferon-stimulated gene (ISG) response by modulating the host's immune signaling cascades. NS4B directly interacts with proteins such as NS4A and NS5 contributing towards the viral RNA polymerase activity. Its interactions stabilize the replication complex enhancing RNA genome synthesis. By inhibiting the unfolded protein response NS4B affects protein homeostasis allowing the virus to continue exploiting host resources.

NS4B's function relates to Zika virus infection and it has associations with microcephaly in newborns. The protein plays a role in the virus’s pathogenic effects especially in neural cells contributing to neurodevelopmental disorders. Additionally NS4B impacts Guillain-Barré syndrome development linking it with immune-mediated damage to peripheral nerves. The interaction of NS4B with other proteins like NS1 helps modulate immune evasion strategies enhancing disease progression and infection severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Zika virus NS4B protein
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Product promise

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