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AB47116

Anti-Zinc Alpha 2 Glycoprotein antibody

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(2 Publications)

Rabbit Polyclonal Zinc Alpha 2 Glycoprotein antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AZGP1 aa 1 to C-terminus.

View Alternative Names

ZAG, ZNGP1, AZGP1, Zinc-alpha-2-glycoprotein, Zn-alpha-2-GP, Zn-alpha-2-glycoprotein

2 Images
Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (AB47116)
  • WB

Unknown

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (AB47116)

All lanes:

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (ab47116) at 1 µg/mL

Lane 1:

Human Plasma Total Protein Lysate at 10 µg

Lane 2:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 3:

Human testis tissue lysate - total protein (<a href='/en-us/products/unavailable/human-testis-tissue-lysate-total-protein-ab30257'>ab30257</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 34 kDa

Observed band size: 43 kDa,55 kDa

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Exposure time: 30s

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (AB47116)
  • WB

CiteAb

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody (AB47116)

Zinc Alpha 2 Glycoprotein western blot using anti-Zinc Alpha 2 Glycoprotein antibody ab47116. Publication image and figure legend from Shetty, V., Hafner, J., et al., 2012, Clin Proteomics, PubMed 22856521.

ab47116 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab47116 please see the product overview.

Western blot analysis : a) Validation of differentially sialylated glycoproteins b) Investigation of O-sialylated glycoprotein contribution c) Pooling vs individual patient sample analysis.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human AZGP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25311

Specificity

This antibody is specific for Zinc Alpha 2 Glycoprotein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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AB277419

Human Zinc Alpha 2 Glycoprotein ELISA kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Immunoaffinity chromatography on a column with immobilized recombinant human Zinc Alpha 2 Glycoprotein.
Storage buffer
Constituents: 0.58% Sodium chloride, 0.134% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc Alpha 2 Glycoprotein (ZAG) also called ZAG or AZGP1 is a soluble protein with a molecular mass of approximately 41 kDa. It functions as an adipokine and is mostly found in the liver breast and prostate tissues as well as in bodily fluids like plasma and saliva. ZAG belongs to the major histocompatibility complex class I family and it shares structural similarities with antigen-presenting proteins.
Biological function summary

Zinc Alpha 2 Glycoprotein assists in lipid metabolism. It promotes the breakdown of triglycerides in adipose tissue by stimulating the beta-adrenergic pathway. ZAG functions independently and does not typically form complexes with other proteins during this process. Its presence in adipose tissue suggests a role in energy homeostasis and body weight regulation.

Pathways

Zinc Alpha 2 Glycoprotein participates in the metabolic pathway for lipid degradation. The protein interacts with beta-3 adrenergic receptors to trigger lipolysis which involves breaking down fat cells into free fatty acids and glycerol. Its action in this pathway links ZAG with other proteins such as perilipin which protects lipid droplets from breakdown.

Zinc Alpha 2 Glycoprotein has connections with metabolic-related conditions notably obesity and cancer cachexia. In obesity lower levels of ZAG are often observed potentially linking it to impaired fat metabolism. In cancer cachexia elevated ZAG levels are frequently detected suggesting a role in the loss of fat and muscle tissue. ZAG's interaction with certain cytokines may enhance cachexia progression by affecting protein degradation pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
See full target information AZGP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 9:10 PubMed22856521

2012

Investigation of ovarian cancer associated sialylation changes in N-linked glycopeptides by quantitative proteomics.

Applications

WB

Species

Unspecified reactive species

Vivekananda Shetty,Julie Hafner,Punit Shah,Zacharie Nickens,Ramila Philip

Journal of proteome research 9:227-36 PubMed19921957

2009

Tandem 18O stable isotope labeling for quantification of N-glycoproteome.

Applications

WB

Species

Human

Ze Liu,Jing Cao,Yifeng He,Liang Qiao,Congjian Xu,Haojie Lu,Pengyuan Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB180574

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]

primary-antibodies

zinc-alpha-2-glycoprotein-antibody-epr14425b-ab180574

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