AB250225

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Zinc Alpha 2 Glycoprotein antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ZAG, ZNGP1, AZGP1, Zinc-alpha-2-glycoprotein, Zn-alpha-2-GP, Zn-alpha-2-glycoprotein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Human liver tissue labeling Zinc Alpha 2 Glycoprotein with ab180574 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Immunoprecipitation of Human plasma labeling Zinc Alpha 2 Glycoprotein using ab180574at a 1/30 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (<a href='/en-us/products/primary-antibodies/zinc-alpha-2-glycoprotein-antibody-epr14425b-ab180574'>ab180574</a>) at 1/30 dilution

Lane 1:

Human plasma

Lane 2:

Negative control

Secondary

All lanes:

Anti-Rabbit (HRP) specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] (<a href='/en-us/products/primary-antibodies/zinc-alpha-2-glycoprotein-antibody-epr14425b-ab180574'>ab180574</a>) at 1/20000 dilution

All lanes:

Human plasma at 20 µg

Secondary

All lanes:

Anti-rabbit (HRP) specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

This data was developed using ab180574, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-rabbit HRP at 1/1000 dilution

Predicted band size: 34 kDa

false

Unconjugated

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14425(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250225 is the carrier-free version of ab180574.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc Alpha 2 Glycoprotein (ZAG) also called ZAG or AZGP1 is a soluble protein with a molecular mass of approximately 41 kDa. It functions as an adipokine and is mostly found in the liver breast and prostate tissues as well as in bodily fluids like plasma and saliva. ZAG belongs to the major histocompatibility complex class I family and it shares structural similarities with antigen-presenting proteins.

Biological function summary

Zinc Alpha 2 Glycoprotein assists in lipid metabolism. It promotes the breakdown of triglycerides in adipose tissue by stimulating the beta-adrenergic pathway. ZAG functions independently and does not typically form complexes with other proteins during this process. Its presence in adipose tissue suggests a role in energy homeostasis and body weight regulation.

Pathways

Zinc Alpha 2 Glycoprotein participates in the metabolic pathway for lipid degradation. The protein interacts with beta-3 adrenergic receptors to trigger lipolysis which involves breaking down fat cells into free fatty acids and glycerol. Its action in this pathway links ZAG with other proteins such as perilipin which protects lipid droplets from breakdown.

Zinc Alpha 2 Glycoprotein has connections with metabolic-related conditions notably obesity and cancer cachexia. In obesity lower levels of ZAG are often observed potentially linking it to impaired fat metabolism. In cancer cachexia elevated ZAG levels are frequently detected suggesting a role in the loss of fat and muscle tissue. ZAG's interaction with certain cytokines may enhance cachexia progression by affecting protein degradation pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.

See full target information AZGP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

Immunoprecipitation - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

Western blot - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR14425(B)] - BSA and Azide free (AB250225)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise