AB281256

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR22380-229] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Zinc Alpha 2 Glycoprotein antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

ZAG, ZNGP1, AZGP1, Zinc-alpha-2-glycoprotein, Zn-alpha-2-GP, Zn-alpha-2-glycoprotein

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Sandwich ELISA - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR22380-229] - BSA and Azide free (Capture) (AB281256)

sELISA

Lab

Sandwich ELISA - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR22380-229] - BSA and Azide free (Capture) (AB281256)

Sandwich ELISA of ab253696 with the capture antibody ab281256 dilution at 2 µg/mL and detector antibody ab281106 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22380-229

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab281256 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human AZGP1. The recommended pair for sandwich ELISA is:
Capture: ab281256, Human AZGP1 Capture Antibody (unconjugated)
Detector: ab281106, Human AZGP1 Detector Antibody (unconjugated)
The reference range value is 39.06 - 5000 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc Alpha 2 Glycoprotein (ZAG) also called ZAG or AZGP1 is a soluble protein with a molecular mass of approximately 41 kDa. It functions as an adipokine and is mostly found in the liver breast and prostate tissues as well as in bodily fluids like plasma and saliva. ZAG belongs to the major histocompatibility complex class I family and it shares structural similarities with antigen-presenting proteins.

Biological function summary

Zinc Alpha 2 Glycoprotein assists in lipid metabolism. It promotes the breakdown of triglycerides in adipose tissue by stimulating the beta-adrenergic pathway. ZAG functions independently and does not typically form complexes with other proteins during this process. Its presence in adipose tissue suggests a role in energy homeostasis and body weight regulation.

Pathways

Zinc Alpha 2 Glycoprotein participates in the metabolic pathway for lipid degradation. The protein interacts with beta-3 adrenergic receptors to trigger lipolysis which involves breaking down fat cells into free fatty acids and glycerol. Its action in this pathway links ZAG with other proteins such as perilipin which protects lipid droplets from breakdown.

Zinc Alpha 2 Glycoprotein has connections with metabolic-related conditions notably obesity and cancer cachexia. In obesity lower levels of ZAG are often observed potentially linking it to impaired fat metabolism. In cancer cachexia elevated ZAG levels are frequently detected suggesting a role in the loss of fat and muscle tissue. ZAG's interaction with certain cytokines may enhance cachexia progression by affecting protein degradation pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.

See full target information AZGP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zinc Alpha 2 Glycoprotein antibody [EPR22380-229] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-Zinc Alpha 2 Glycoprotein antibody [EPR22380-229] - BSA and Azide free (Capture) (AB281256)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise