Anti-Zinc finger antiviral protein antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody (AB154680)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ZC3HAV1 protein at cytoplasm using ab154680 at a 1/1000 dilution (Green). DAPI was used for nuclear staining (Blue). Scale bar = 10 µm

• WB

Supplier Data

Western blot - Anti-Zinc finger antiviral protein antibody (AB154680)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Zinc finger antiviral protein antibody (ab154680) at 1/10000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 101 kDa

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• WB

CiteAb

Western blot - Anti-Zinc finger antiviral protein antibody (AB154680)

Western Blotting using Anti-Zinc finger antiviral protein antibody, ab154680. Publication image from Odon, V. et al., 2019, Nucleic Acids Res, 31276592. Legend direct from paper.

Binding of affinity isolated ZAP to CpG-H and UpA-H RNA transcripts. (A) ZAP detection by WB in input A549 cell lysates before and after immunoprecipitation (IP) with anti-ZAP or irrelevant antibody control (lower panel; IP). Long and short isoforms of ZAP migrated at around 90 and 75 KDa, respectively. The negative control was mock transfected (no viral RNA control). (B) Quantitation of E7 viral RNA transcripts with WT, CpG-high and UpA-high R2 sequences by qPCR in immunoprecipitated ZAP (right column) or mock precipitated control (left column). Bar heights show the mean of two biological experiments, errors bars show standard errors of the mean. The no RNA transcript control was negative by qPCR (data not shown).

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• WB

CiteAb

Western blot - Anti-Zinc finger antiviral protein antibody (AB154680)

Western Blotting using Anti-Zinc finger antiviral protein antibody, ab154680. Publication image from Odon, V. et al., 2019, Nucleic Acids Res, 31276592. Legend direct from paper.

Expression of ZAP, RNAseL and OAS3 in cell lines; association of ZAP expression with attenuation. Detection of ZAP-L and ZAP-S (A) and RNAseL, and OAS3 (B) by western blot analysis of lysates of different cell lines before and after stimulation with exogenous IFN-β. (C) Association of ZAP-L expression with the attenuation index of mutants of E7 with high CpG or UpA 3′UTR sequences. The black and grey dotted lines show lines of best fit and associated R values. Significance levels were based on one-tailed probabilities using the Spearman non-parametric test.

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