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Rabbit Recombinant Monoclonal Zinc finger antiviral protein antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZC3HAV1.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (AB313866), expandable thumbnail
  • Western blot - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (AB313866), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (AB313866), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ZC3HAV1. Database link Q7Z2W4

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Antiviral protein which inhibits the replication of viruses by recruiting the cellular RNA degradation machineries to degrade the viral mRNAs. Binds to a ZAP-responsive element (ZRE) present in the target viral mRNA, recruits cellular poly(A)-specific ribonuclease PARN to remove the poly(A) tail, and the 3'-5' exoribonuclease complex exosome to degrade the RNA body from the 3'-end. It also recruits the decapping complex DCP1-DCP2 through RNA helicase p72 (DDX17) to remove the cap structure of the viral mRNA to initiate its degradation from the 5'-end. Its target viruses belong to families which include retroviridae: human immunodeficiency virus type 1 (HIV-1), moloney and murine leukemia virus (MoMLV) and xenotropic MuLV-related virus (XMRV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), togaviridae: sindbis virus (SINV) and Ross river virus (RRV). Specifically targets the multiply spliced but not unspliced or singly spliced HIV-1 mRNAs for degradation. Isoform 1 is a more potent viral inhibitor than isoform 2. Isoform 2 acts as a positive regulator of RIGI signaling resulting in activation of the downstream effector IRF3 leading to the expression of type I IFNs and IFN stimulated genes (ISGs).

Alternative names

ZC3HDC2, PRO1677, ZC3HAV1, Zinc finger CCCH-type antiviral protein 1, ADP-ribosyltransferase diphtheria toxin-like 13, Inactive Poly [ADP-ribose] polymerase 13, Zinc finger CCCH domain-containing protein 2, Zinc finger antiviral protein, ARTD13, PARP13, ZAP

Recommended products

Rabbit Recombinant Monoclonal Zinc finger antiviral protein antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZC3HAV1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human ZC3HAV1. Database link Q7Z2W4
Carrier free
Yes
Clone number
HL1884
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Zinc finger antiviral protein (ZAP) also known as zinc antiviral is a cellular protein that plays an important role in antiviral defense. ZAP recognizes and binds to the CpG dinucleotide-rich regions of viral RNA targeting them for degradation and preventing viral replication. This protein has a molecular mass of around 100 kDa and is encoded by the ZC3HAV1 gene. ZAP predominantly expresses in the cytoplasm of various cells notably in immune cells where it carries out its antiviral function against a wide range of viruses.

Biological function summary

Zinc finger antiviral protein serves a critical role in the innate immune response. ZAP is part of a complex involving several cofactors that facilitate its antiviral activity. This protein collaborates with TRIM25 and AGO2 to recruit RNA degradation machinery targeting viral mRNA for decay. ZAP's interactions highlight its importance in the cellular response to viral infection making it an attractive target for developing antiviral therapies.

Pathways

Zinc finger antiviral protein is integral to the interferon signaling and viral RNA degradation pathways. Interferon signaling initiates the expression of ZAP linking it with other interferon-stimulated genes to enhance the antiviral response. Within the RNA degradation pathway ZAP coordinates with proteins like TRIM25 augmenting the destruction of viral genetic material. These pathways highlight the protein's role in suppressing viral replication and promoting cell survival during infections.

Associated diseases and disorders

Zinc finger antiviral protein plays a significant role in conditions associated with viral infections and autoimmune disorders. ZAP confers protection against diseases like Hepatitis B and C by inhibiting viral RNA. Apart from infectious diseases ZAP interacts with proteins like HRAS and IRF3 suggesting its involvement in autoimmune conditions where viral components trigger immune responses. This relationship opens possibilities for therapeutic intervention by modulating ZAP activity in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (ab313866), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (ab313866)

    Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells labelling ZC3HAV1 protein at cytoplasm using ab313866 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).

  • Western blot - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (ab313866), expandable thumbnail

    Western blot - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (ab313866)

    All lanes: Western blot - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (ab313866) at 1/1000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 101 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (ab313866), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger antiviral protein antibody [HL1884] - BSA and Azide free (ab313866)

    Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling ZC3HAV1 with ab313866 at 1/500 dilution (green). Blue: Fluoroshield with DAPI. Scale bar= 10um.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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