Rabbit Polyclonal Zinc finger MIZ domain-containing protein 1 antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZMIZ1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ZMIZ1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULJ6
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Target data
Function
Acts as a transcriptional coactivator. Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation (PubMed:14609956, PubMed:26522984). Also functions as a transcriptional coactivator in the TGF-beta signaling pathway by increasing the activity of the SMAD3/SMAD4 transcriptional complex (PubMed:16777850). Involved in transcriptional activation of a subset of NOTCH1 target genes including MYC. Involved in thymocyte and T cell development (By similarity). Involved in the regulation of postmitotic positioning of pyramidal neurons in the developing cerebral cortex (PubMed:30639322).
Alternative names
KIAA1224, RAI17, ZIMP10, ZMIZ1, Zinc finger MIZ domain-containing protein 1, PIAS-like protein Zimp10, Retinoic acid-induced protein 17
Recommended products
Rabbit Polyclonal Zinc finger MIZ domain-containing protein 1 antibody. N-terminal. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZMIZ1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ZMIZ1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULJ6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zinc finger MIZ domain-containing protein 1 also known as ZMIZ1 acts mechanically as a transcriptional co-activator. It has a molecular weight of approximately 135 kDa. ZMIZ1 contains a zinc finger domain which is a typical feature for proteins that interact with DNA. This protein is expressed in a variety of tissues including the brain and immune system suggesting diverse roles in cellular processes. The zinc finger MIZ domain facilitates interaction with other proteins and allows it attachment to DNA enabling regulation of transcriptional activity.
- Biological function summary
ZMIZ1 influences gene expression by binding with other transcription factors including androgen receptor and Smad proteins to regulate DNA transcription. It is part of protein complexes that modulate various signaling pathways impacting cellular growth and differentiation. The involvement in such complexes reveals its role in fine-tuning transcription processes required for normal cellular functions. The co-activation function of ZMIZ1 can influence the expression of genes that are important for both embryonic and hematopoietic development.
- Pathways
ZMIZ1 integrates within the TGF-beta signaling and androgen receptor pathways. In the TGF-beta pathway ZMIZ1 works alongside Smad proteins to enhance the transcriptional responses helping control cell proliferation and apoptosis. In the androgen receptor pathway it aids androgen receptor to activate target gene transcription. These interactions position ZMIZ1 as a central player in regulating processes controlled by those pathways potentially influencing cellular development and oncogenesis.
- Associated diseases and disorders
ZMIZ1 associates with cancer and autoimmune diseases. Research shows its overexpression in certain cancers such as prostate cancer where it might amplify androgen receptor signaling contributing to cancer progression. The protein's connection to autoimmune disorders involves interactions with other immune-related proteins which when dysregulated can lead to improper immune responses. Understanding ZMIZ1 signaling better might offer insights into therapeutic strategies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Zinc finger MIZ domain-containing protein 1 antibody - N-terminal (ab200376)
6% SDS-PAGE
All lanes: Western blot - Anti-Zinc finger MIZ domain-containing protein 1 antibody - N-terminal (ab200376) at 1/200 dilution
All lanes: Skov3 cell lysate at 40 µg
Predicted band size: 115 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zinc finger MIZ domain-containing protein 1 antibody - N-terminal (ab200376)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Zinc finger MIZ domain-containing protein 1 with ab200376 at 1/40 dilution.
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