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AB254868

Anti-ZIP-3 antibody

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(4 Publications)

Rabbit Polyclonal ZIP-3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC39A3 aa 100-200.

View Alternative Names

ZIP3, SLC39A3, Zinc transporter ZIP3, Solute carrier family 39 member 3, Zrt- and Irt-like protein 3, ZIP-3

3 Images
Western blot - Anti-ZIP-3 antibody (AB254868)
  • WB

Supplier Data

Western blot - Anti-ZIP-3 antibody (AB254868)

All lanes:

Western blot - Anti-ZIP-3 antibody (ab254868) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

ZIP-3 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 34 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-ZIP-3 antibody (AB254868)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZIP-3 antibody (AB254868)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling ZIP-3 using ab254868 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-3 antibody (AB254868)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-3 antibody (AB254868)

Paraffin-embedded human colon tissue stained for ZIP-3 using ab254868 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLC39A3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BRY0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZIP-3 also known as SLC39A3 is a protein that functions as a zinc transporter facilitating the uptake of zinc into cells. This protein belongs to the solute carrier (SLC) family and typically has a mass of approximately 47 kDa. ZIP-3 localizes mainly to the plasma membrane and is expressed in a variety of tissues including liver kidney and intestine. It plays an important role in maintaining intracellular zinc homeostasis critical for various cellular functions.
Biological function summary

Zinc is essential for numerous cellular processes and ZIP-3 helps regulate its availability. Zinc acts as a cofactor for over 300 enzymes and plays a vital role in transcription factor function gene expression and cell signaling. Through these actions ZIP-3 impacts cellular proliferation differentiation and survival. ZIP-3 does not form part of a larger complex but works closely with other zinc transporters such as those from the ZIP and ZnT families to balance zinc levels in the body.

Pathways

Researchers have associated ZIP-3 with critical processes such as the metal ion transport and cellular response to stress. By modulating zinc levels ZIP-3 interacts with pathways like the MAPK signaling pathway which is pivotal for cell growth and division. In these pathways ZIP-3 operates alongside proteins like ZIP-4 and ZnT-1 ensuring proper zinc redistribution and cellular adaptation to changing environmental conditions.

Scientists have linked ZIP-3 dysfunction to conditions such as type 2 diabetes and neurodegenerative disorders. Abnormal ZIP-3 activity can disrupt zinc homeostasis contributing to insulin resistance in diabetes. In neurodegenerative diseases impaired zinc transport by ZIP-3 can lead to neuronal damage and cognitive deficits. Research also examines the interplay of ZIP-3 with other proteins such as insulin receptor substrate (IRS) in diabetes highlighting the potential for targeted therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transporter for the divalent cation Zn(2+). Mediates the influx of Zn(2+) into cells from extracellular space. Controls Zn(2+) accumulation into dentate gyrus granule cells in the hippocampus. Mediates Zn(2+) reuptake from the secreted milk within the alveolar lumen.
See full target information SLC39A3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal science 102: PubMed39031082

2024

Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Hu,Wei Wu,Liang Huang,Liyang Zhang,Chunyu Cao,Weiyun Zhang,Yangyang Hu,Xiaoyan Cui,Tingting Li,Shengchen Wang,Xugang Luo

Poultry science 102:102477 PubMed36680861

2023

The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Hu,Yanhui Huang,Chuanlong Wang,Weiyun Zhang,Yicheng Qu,Ding Li,Wei Wu,Feiyu Gao,Ling Zhu,Bingxin Wu,Liyang Zhang,Xiaoyan Cui,Tingting Li,Yanqiang Geng,Xiudong Liao,Xugang Luo

Frontiers in physiology 13:952941 PubMed35936908

2022

Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Hu,Chuanlong Wang,Wei Wu,Yicheng Qu,Weiyun Zhang,Ding Li,Ling Zhu,Feiyu Gao,Bingxin Wu,Liyang Zhang,Xiaoyan Cui,Tingting Li,Yanqiang Geng,Xugang Luo

Molecular medicine reports 25: PubMed35419615

2022

SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Cai,Zhenhua Sun,Lili Zhang,Hongrui Xu
View all publications
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