Rabbit Polyclonal ZIP-4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC39A4 aa 1-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SLC39A4 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P5W5
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Selective transporter that mediates the uptake of Zn(2+) (PubMed:17202136, PubMed:22242765, PubMed:27321477, PubMed:28875161, PubMed:31164399, PubMed:31914589, PubMed:31979155, PubMed:33837739, PubMed:36473915). Plays an essential role for dietary zinc uptake from small intestine (By similarity). The Zn(2+) uniporter activity is regulated by zinc availability (PubMed:17202136, PubMed:32348750). Exhibits also polyspecific binding and transport of Cu(2+), Cd(2+) and possibly Ni(2+) but at higher concentrations (PubMed:22242765, PubMed:31914589).
Alternative names
ZIP4, SLC39A4, Zinc transporter ZIP4, Solute carrier family 39 member 4, Zrt- and Irt-like protein 4, ZIP-4
Recommended products
Rabbit Polyclonal ZIP-4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC39A4 aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SLC39A4 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P5W5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZIP-4 also known as SLC39A4 functions as a zinc transporter responsible for the uptake of zinc ions from the extracellular space into the cytoplasm. It plays a critical role in maintaining zinc homeostasis within the cell. The protein has a molecular mass of approximately 67 kDa. ZIP-4 is predominantly expressed in the small intestine and pancreas where it regulates zinc absorption important for various biological processes.
- Biological function summary
ZIP-4 influences the cellular zinc levels by facilitating zinc influx across the plasma membrane. This transport ensures adequate zinc supply for enzymatic reactions and cellular functions. ZIP-4 does not operate as part of a larger complex but acts independently to regulate zinc entry which impacts cell signaling immune responses and cell proliferation.
- Pathways
ZIP-4 participates in the regulation of zinc-dependent pathways such as the MAPK signaling pathway and the NF-kB signaling pathway. These pathways are essential for cell growth survival and immune response. ZIP-4 interacts with other transporters like ZIP-1 and ZIP-10 contributing significantly to the intricate network of zinc homeostasis and cellular regulation.
- Associated diseases and disorders
Abnormal ZIP-4 function has links to acrodermatitis enteropathica a rare genetic disorder caused by impaired zinc absorption. This condition causes skin lesions immune dysfunctions and developmental issues. Additionally dysregulation of ZIP-4 has been implicated in certain cancers where increased ZIP-4 expression correlates with tumor growth and metastasis highlighting its relevance in disease pathology and potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-ZIP-4 antibody (ab230099)
All lanes: Western blot - Anti-ZIP-4 antibody (ab230099) at 1/1000 dilution
Lane 1: Mouse stomach lysate
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-4 antibody (ab230099)
Paraffin-embedded human kidney tissue stained for ZIP-4 using ab230099 at 1/100 dilution in immunohistochemical analysis.
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