AB105194

Anti-ZIP-5 antibody

Name: Anti-ZIP-5 antibody (ab105194) | Abcam
Brand: Abcam Limited
SKU: AB105194

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(4 Publications)

Rabbit Polyclonal ZIP-5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SLC39A5.

View Alternative Names

ZIP5, SLC39A5, Zinc transporter ZIP5, Solute carrier family 39 member 5, Zrt- and Irt-like protein 5, ZIP-5

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-5 antibody (AB105194)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-5 antibody (AB105194)

Immunohistochemistry of ZIP-5 in Human spleen tissue with ab105194 at 2.5 μg/mL.

Immunocytochemistry/ Immunofluorescence - Anti-ZIP-5 antibody (AB105194)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZIP-5 antibody (AB105194)

Immunofluorescence of ZIP-5 in Human spleen tissue with ab105194 at 20 ug/mL.

Unknown

Western blot - Anti-ZIP-5 antibody (AB105194)

All lanes:

Western blot - Anti-ZIP-5 antibody (ab105194) at 1 µg/mL

All lanes:

Human spleen tissue lysate at 15 µg

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SLC39A5. The exact immunogen used to generate this antibody is proprietary information.

Q6ZMH5

Specificity

ab105194 is predicted not to cross-react with other ZIP family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZIP-5 also known as SLC39A5 functions as a zinc transporter responsible for importing zinc ions into cells. This protein plays a critical role in maintaining zinc homeostasis allowing cells to efficiently uptake zinc from the extracellular environment. ZIP-5 has a mass of approximately 74 kDa. It is prominently expressed in tissues involved in zinc metabolism such as the pancreas liver and intestine. The location of its expression suggests its significant involvement in the regulation of zinc availability in important metabolic processes.

Biological function summary

The ZIP-5 protein actively facilitates zinc uptake necessary for numerous cellular processes like enzymatic activity and gene expression. ZIP-5 does not act alone; it can interact with other proteins to ensure precise zinc distribution within cells. The function of ZIP-5 in cellular zinc homeostasis is essential for maintaining normal cell function and preventing zinc-related cytotoxicity which can disrupt various cellular activities.

Pathways

ZIP-5 participates in the metallothionein and metal response element-binding transcription factor 1 (MTF-1) pathway. This pathway is important for zinc sensing and regulation. ZIP-5 operates in network with other zinc transporters such as ZIP4 to finely tune intracellular zinc levels. Through these interactions ZIP-5 adjusts cellular zinc levels during metabolic activities ensuring that zinc is available for necessary biochemical pathways like those that regulate cell growth differentiation and apoptosis.

ZIP-5 is implicated in conditions involving zinc deficiency or misregulation such as acrodermatitis enteropathica and chronic liver disease. For instance its altered expression may contribute to zinc malabsorption associated with such conditions. Additionally interactions between ZIP-5 and ZIP4 suggest an overlapping role in these disorders where proper functioning of both proteins is required to maintain zinc homeostasis. Understanding ZIP-5's role in these diseases aids in unraveling its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Uniporter that transports zinc(2+) into polarized cells of enterocytes, pancreatic acinar and endoderm cells across the basolateral membrane and participates, notably, in zinc excretion from the intestine by the uptake of zinc from the blood into the intestine (By similarity). The transport mechanism is temperature- and concentration-dependent and saturable (By similarity). In addition, is also a high affinity copper transporter in vitro (PubMed : 36454509). Also may regulate glucose-stimulated insulin secretion (GSIS) in islets primarily through the zinc-activated SIRT1-PPARGC1A axis (By similarity). Could regulate the BMP/TGF-beta (bone morphogenetic protein/transforming growth factor-beta) signaling pathway and modulates extracellular matrix (ECM) proteins of the sclera (PubMed : 24891338). Plays a role in eye development (PubMed : 24891338).

See full target information SLC39A5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of animal science 102: PubMed39031082

2024

Zinc proteinate with moderate chelation strength enhances zinc absorption by upregulating the expression of zinc and amino acid transporters in primary cultured duodenal epithelial cells of broiler embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Hu,Wei Wu,Liang Huang,Liyang Zhang,Chunyu Cao,Weiyun Zhang,Yangyang Hu,Xiaoyan Cui,Tingting Li,Shengchen Wang,Xugang Luo

Poultry science 102:102477 PubMed36680861

2023

The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Hu,Yanhui Huang,Chuanlong Wang,Weiyun Zhang,Yicheng Qu,Ding Li,Wei Wu,Feiyu Gao,Ling Zhu,Bingxin Wu,Liyang Zhang,Xiaoyan Cui,Tingting Li,Yanqiang Geng,Xiudong Liao,Xugang Luo

Frontiers in physiology 13:952941 PubMed35936908

2022

Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Hu,Chuanlong Wang,Wei Wu,Yicheng Qu,Weiyun Zhang,Ding Li,Ling Zhu,Feiyu Gao,Bingxin Wu,Liyang Zhang,Xiaoyan Cui,Tingting Li,Yanqiang Geng,Xugang Luo

International journal of biological sciences 16:1481-1494 PubMed32226296

2020

Inflammation-dependent downregulation of miR-532-3p mediates apoptotic signaling in human sarcopenia through targeting .

Applications

Unspecified application

Species

Unspecified reactive species

Fa-Xiu Chen,Yi Shen,Yang Liu,Hai-Feng Wang,Chen-Yu Liang,Ming Luo

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ZIP-5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-5 antibody (AB105194)

Immunocytochemistry/ Immunofluorescence - Anti-ZIP-5 antibody (AB105194)

Western blot - Anti-ZIP-5 antibody (AB105194)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

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