Rabbit Polyclonal ZIP-9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Zinc transporter ZIP9 aa 100-200.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Predicted | Predicted |
Rat | Predicted | Predicted |
Chicken | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken | Dilution info - | Notes - |
Transports zinc ions across cell and organelle membranes into the cytoplasm and regulates intracellular zinc homeostasis (PubMed:19420709, PubMed:25014355, PubMed:28219737). Participates in the zinc ions efflux out of the secretory compartments (PubMed:19420709). Regulates intracellular zinc level, resulting in the enhancement of AKT1 and MAPK3/MAPK1 (Erk1/2) phosphorylation in response to the BCR activation (PubMed:23505453). Also functions as a membrane androgen receptor that mediates, through a G protein, the non-classical androgen signaling pathway, characterized by the activation of MAPK3/MAPK1 (Erk1/2) and transcription factors CREB1 or ATF1 (By similarity). This pathway contributes to CLDN1 and CLDN5 expression and tight junction formation between adjacent Sertoli cells (By similarity). Mediates androgen-induced vascular endothelial cell proliferation through activation of an inhibitory G protein leading to the AKT1 and MAPK3/MAPK1 (Erk1/2) activation which in turn modulate inhibition (phosphorylation) of GSK3B and CCND1 transcription (PubMed:34555425). Moreover, has dual functions as a membrane-bound androgen receptor and as an androgen-dependent zinc transporter both of which are mediated through an inhibitory G protein (Gi) that mediates both MAP kinase and zinc signaling leading to the androgen-dependent apoptotic process (PubMed:25014355, PubMed:28219737).
ZIP9, UNQ714/PRO1377, SLC39A9, ZIP9, UNQ714/PRO1377, Zinc transporter ZIP9, Solute carrier family 39 member 9, Zrt- and Irt-like protein 9, ZIP-9
Rabbit Polyclonal ZIP-9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Zinc transporter ZIP9 aa 100-200.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
ZIP-9 also known as SLC39A9 is a member of the zinc transporter family that plays an important role in regulating zinc homeostasis. It functions by facilitating the influx of zinc from extracellular space into the cytoplasm. ZIP-9 with a molecular mass of approximately 35 kDa is expressed in various tissues including the liver kidney and reproductive organs. It possesses a characteristic structure with eight transmembrane domains and is localized primarily in the plasma membrane and the endoplasmic reticulum.
ZIP-9 acts as a membrane androgen receptor and is involved in non-genomic androgen signaling. It does not typically form part of a larger complex but its activity greatly influences cellular zinc levels and hormone signaling pathways. ZIP-9 triggers rapid signaling events upon androgen binding which may affect multiple cellular processes including apoptosis proliferation and differentiation indicating its role in cellular response to hormonal and nutritional changes.
Zinc is a central element in cellular signaling processes which involve ZIP-9 particularly in the MAPK and PI3K-Akt signaling pathways. ZIP-9's role in zinc transport affects these pathways by regulating intracellular zinc concentrations which in turn can influence downstream signaling events. ZIP-9 interactions with other proteins such as ZIP4 in the zinc transporter family impact zinc-mediated signaling dynamics and cellular homeostasis.
Abnormal ZIP-9 function has been associated with prostate cancer and polycystic ovary syndrome (PCOS). In prostate cancer ZIP-9's role in androgen signaling enhances tumor cell growth and proliferation linking it to androgen receptor pathways. Likewise in PCOS disturbances in zinc metabolism through ZIP-9 can contribute to the hormonal imbalances observed in this disorder. Studying ZIP-9 interactions particularly with androgen receptors may provide insights into therapeutic strategies for these conditions.
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Paraffin-embedded formalin-fixed human thyroid tissue stained for ZIP-9 using ab272557 at 1/50 dilution in immunohistochemical analysis.
All lanes: Western blot - Anti-ZIP-9 antibody (ab272557) at 0.4 µg/mL
Lane 1: Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2: ZIP-9 over-expression HEK293T lysate
Predicted band size: 32 kDa
Paraffin-embedded formalin-fixed human pancreas tissue stained for ZIP-9 using ab272557 at 1/50 dilution in immunohistochemical analysis. Shows low expression as expected.
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