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AB272557

Anti-ZIP-9 antibody

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Rabbit Polyclonal ZIP-9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC39A9 aa 100-200.

View Alternative Names

ZIP9, UNQ714/PRO1377, SLC39A9, Zinc transporter ZIP9, Solute carrier family 39 member 9, Zrt- and Irt-like protein 9, ZIP-9

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (AB272557)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (AB272557)

Paraffin-embedded formalin-fixed human thyroid tissue stained for ZIP-9 using ab272557 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (AB272557)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (AB272557)

Paraffin-embedded formalin-fixed human pancreas tissue stained for ZIP-9 using ab272557 at 1/50 dilution in immunohistochemical analysis. Shows low expression as expected.

Western blot - Anti-ZIP-9 antibody (AB272557)
  • WB

Unknown

Western blot - Anti-ZIP-9 antibody (AB272557)

All lanes:

Western blot - Anti-ZIP-9 antibody (ab272557) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

ZIP-9 over-expression HEK293T lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC39A9 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NUM3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZIP-9 also known as SLC39A9 is a member of the zinc transporter family that plays an important role in regulating zinc homeostasis. It functions by facilitating the influx of zinc from extracellular space into the cytoplasm. ZIP-9 with a molecular mass of approximately 35 kDa is expressed in various tissues including the liver kidney and reproductive organs. It possesses a characteristic structure with eight transmembrane domains and is localized primarily in the plasma membrane and the endoplasmic reticulum.
Biological function summary

ZIP-9 acts as a membrane androgen receptor and is involved in non-genomic androgen signaling. It does not typically form part of a larger complex but its activity greatly influences cellular zinc levels and hormone signaling pathways. ZIP-9 triggers rapid signaling events upon androgen binding which may affect multiple cellular processes including apoptosis proliferation and differentiation indicating its role in cellular response to hormonal and nutritional changes.

Pathways

Zinc is a central element in cellular signaling processes which involve ZIP-9 particularly in the MAPK and PI3K-Akt signaling pathways. ZIP-9's role in zinc transport affects these pathways by regulating intracellular zinc concentrations which in turn can influence downstream signaling events. ZIP-9 interactions with other proteins such as ZIP4 in the zinc transporter family impact zinc-mediated signaling dynamics and cellular homeostasis.

Abnormal ZIP-9 function has been associated with prostate cancer and polycystic ovary syndrome (PCOS). In prostate cancer ZIP-9's role in androgen signaling enhances tumor cell growth and proliferation linking it to androgen receptor pathways. Likewise in PCOS disturbances in zinc metabolism through ZIP-9 can contribute to the hormonal imbalances observed in this disorder. Studying ZIP-9 interactions particularly with androgen receptors may provide insights into therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transports zinc ions across cell and organelle membranes into the cytoplasm and regulates intracellular zinc homeostasis (PubMed : 19420709, PubMed : 25014355, PubMed : 28219737). Participates in the zinc ions efflux out of the secretory compartments (PubMed : 19420709). Regulates intracellular zinc level, resulting in the enhancement of AKT1 and MAPK3/MAPK1 (Erk1/2) phosphorylation in response to the BCR activation (PubMed : 23505453). Also functions as a membrane androgen receptor that mediates, through a G protein, the non-classical androgen signaling pathway, characterized by the activation of MAPK3/MAPK1 (Erk1/2) and transcription factors CREB1 or ATF1 (By similarity). This pathway contributes to CLDN1 and CLDN5 expression and tight junction formation between adjacent Sertoli cells (By similarity). Mediates androgen-induced vascular endothelial cell proliferation through activation of an inhibitory G protein leading to the AKT1 and MAPK3/MAPK1 (Erk1/2) activation which in turn modulate inhibition (phosphorylation) of GSK3B and CCND1 transcription (PubMed : 34555425). Moreover, has dual functions as a membrane-bound androgen receptor and as an androgen-dependent zinc transporter both of which are mediated through an inhibitory G protein (Gi) that mediates both MAP kinase and zinc signaling leading to the androgen-dependent apoptotic process (PubMed : 25014355, PubMed : 28219737).
See full target information SLC39A9
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Product promise

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