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Rabbit Polyclonal ZIP-9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Zinc transporter ZIP9 aa 100-200.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (AB272557), expandable thumbnail
  • Western blot - Anti-ZIP-9 antibody (AB272557), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (AB272557), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Zinc transporter ZIP9 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUM3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Chicken
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/200.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Chicken

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken

Dilution info

-

Notes

-

Target data

Function

Transports zinc ions across cell and organelle membranes into the cytoplasm and regulates intracellular zinc homeostasis (PubMed:19420709, PubMed:25014355, PubMed:28219737). Participates in the zinc ions efflux out of the secretory compartments (PubMed:19420709). Regulates intracellular zinc level, resulting in the enhancement of AKT1 and MAPK3/MAPK1 (Erk1/2) phosphorylation in response to the BCR activation (PubMed:23505453). Also functions as a membrane androgen receptor that mediates, through a G protein, the non-classical androgen signaling pathway, characterized by the activation of MAPK3/MAPK1 (Erk1/2) and transcription factors CREB1 or ATF1 (By similarity). This pathway contributes to CLDN1 and CLDN5 expression and tight junction formation between adjacent Sertoli cells (By similarity). Mediates androgen-induced vascular endothelial cell proliferation through activation of an inhibitory G protein leading to the AKT1 and MAPK3/MAPK1 (Erk1/2) activation which in turn modulate inhibition (phosphorylation) of GSK3B and CCND1 transcription (PubMed:34555425). Moreover, has dual functions as a membrane-bound androgen receptor and as an androgen-dependent zinc transporter both of which are mediated through an inhibitory G protein (Gi) that mediates both MAP kinase and zinc signaling leading to the androgen-dependent apoptotic process (PubMed:25014355, PubMed:28219737).

Alternative names

Recommended products

Rabbit Polyclonal ZIP-9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Zinc transporter ZIP9 aa 100-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Zinc transporter ZIP9 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUM3
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZIP-9 also known as SLC39A9 is a member of the zinc transporter family that plays an important role in regulating zinc homeostasis. It functions by facilitating the influx of zinc from extracellular space into the cytoplasm. ZIP-9 with a molecular mass of approximately 35 kDa is expressed in various tissues including the liver kidney and reproductive organs. It possesses a characteristic structure with eight transmembrane domains and is localized primarily in the plasma membrane and the endoplasmic reticulum.

Biological function summary

ZIP-9 acts as a membrane androgen receptor and is involved in non-genomic androgen signaling. It does not typically form part of a larger complex but its activity greatly influences cellular zinc levels and hormone signaling pathways. ZIP-9 triggers rapid signaling events upon androgen binding which may affect multiple cellular processes including apoptosis proliferation and differentiation indicating its role in cellular response to hormonal and nutritional changes.

Pathways

Zinc is a central element in cellular signaling processes which involve ZIP-9 particularly in the MAPK and PI3K-Akt signaling pathways. ZIP-9's role in zinc transport affects these pathways by regulating intracellular zinc concentrations which in turn can influence downstream signaling events. ZIP-9 interactions with other proteins such as ZIP4 in the zinc transporter family impact zinc-mediated signaling dynamics and cellular homeostasis.

Associated diseases and disorders

Abnormal ZIP-9 function has been associated with prostate cancer and polycystic ovary syndrome (PCOS). In prostate cancer ZIP-9's role in androgen signaling enhances tumor cell growth and proliferation linking it to androgen receptor pathways. Likewise in PCOS disturbances in zinc metabolism through ZIP-9 can contribute to the hormonal imbalances observed in this disorder. Studying ZIP-9 interactions particularly with androgen receptors may provide insights into therapeutic strategies for these conditions.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (ab272557), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (ab272557)

    Paraffin-embedded formalin-fixed human thyroid tissue stained for ZIP-9 using ab272557 at 1/50 dilution in immunohistochemical analysis.

  • Western blot - Anti-ZIP-9 antibody (ab272557), expandable thumbnail

    Western blot - Anti-ZIP-9 antibody (ab272557)

    All lanes: Western blot - Anti-ZIP-9 antibody (ab272557) at 0.4 µg/mL

    Lane 1: Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

    Lane 2: ZIP-9 over-expression HEK293T lysate

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (ab272557), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-9 antibody (ab272557)

    Paraffin-embedded formalin-fixed human pancreas tissue stained for ZIP-9 using ab272557 at 1/50 dilution in immunohistochemical analysis. Shows low expression as expected.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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