AB51602

Anti-ZIP Kinase antibody [EPR1636Y]

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal ZIP Kinase antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ZIPK, DAPK3, Death-associated protein kinase 3, DAP kinase 3, DAP-like kinase, MYPT1 kinase, Zipper-interacting protein kinase, Dlk, ZIP-kinase

4 Images

Lab

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

Lanes 1- 2 : Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab51602 was shown to react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in knockout cell line ab266755 (knockout cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and DAPK3 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

DAPK3 knockout HEK293T cell lysate at 20 µg

Predicted band size: 53 kDa

Observed band size: 53 kDa

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Lab

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

Lanes 1- 2 : Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab51602 was shown to react with ZIP Kinase in wild-type HEK-293T cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab266755 (CRISPR/Cas9 edited cell lysate ab257407) lane below 53kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HEK-293T and DAPK3 CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

DAPK3 CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human DAPK3 (ZIP Kinase) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-dapk3-zip-kinase-knockout-hek-293t-cell-line-ab266755'>ab266755</a>)

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Lab

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : ZIP Kinase knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : A431 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab51602 was shown to specifically react with ZIP Kinase in wild-type HAP1 cells. No band was observed when ZIP Kinase knockout samples were examined. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. ab51602 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (ab51602)

Predicted band size: 53 kDa

false

Unknown

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

All lanes:

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1636Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZIP Kinase also known as DAPK3 is a serine/threonine protein kinase with a molecular mass of approximately 52 kDa. It is expressed in a variety of tissues including the heart liver and skeletal muscle indicating its broad physiological importance. ZIP Kinase shows activity in phosphorylating specific substrates that influence cell structure and function playing a role in cellular processes.

Biological function summary

ZIP Kinase regulates apoptosis and autophagy impacting cell survival and homeostasis. It does not act in isolation but is part of a complex with other kinases and regulatory proteins. ZIP Kinase interacts with cytoskeletal proteins to mediate cell contraction and motility indicating its involvement in both normal cellular dynamics and stress responses.

Pathways

ZIP Kinase is involved in the apoptosis and autophagy pathways. Within the apoptosis pathway ZIP Kinase partners with proteins like DAPK1 influencing stress and injury responses. In the autophagy pathway it helps in regulating cellular degradation processes. These pathways allow ZIP Kinase to integrate signals that manage cell fate decisions collaboratively modulating cellular life and death.

ZIP Kinase associates with hypertension and certain types of cancer. In hypertension ZIP Kinase dysregulation may impact vascular smooth muscle cell contractility due to its connection with myosin light chain phosphorylation. In cancer altered ZIP Kinase activity may affect apoptosis linking it to aberrant cell proliferation. Its interaction with proteins like DAPK1 in both contexts underlines its role in pathological states where cell death and survival are disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

See full target information DAPK3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:13810 PubMed31554851

2019

L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks.

Applications

Unspecified application

Species

Unspecified reactive species

William Plumbly,Nick Brandon,Tarek Z Deeb,Jeremy Hall,Adrian J Harwood

Molecular cancer research : MCR 13:358-67 PubMed25304685

2014

DAPK3 suppresses acini morphogenesis and is required for mouse development.

Applications

Species

Human

Brandon A Kocher,Lynn S White,David Piwnica-Worms

Cancer research 70:1344-55 PubMed20124481

2010

Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens.

Applications

Species

Human

Ovidiu Marina,Ursula Hainz,Melinda A Biernacki,Wandi Zhang,Ann Cai,Jonathan S Duke-Cohan,Fenglong Liu,Vladimir Brusic,Donna Neuberg,Jeffery L Kutok,Edwin P Alyea,Christine M Canning,Robert J Soiffer,Jerome Ritz,Catherine J Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZIP Kinase antibody [EPR1636Y]

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

Western blot - Anti-ZIP Kinase antibody [EPR1636Y] (AB51602)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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