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Rabbit Recombinant Monoclonal ZIP Kinase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (AB210528), expandable thumbnail
  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (AB210528), expandable thumbnail
  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (AB210528), expandable thumbnail
  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (AB210528), expandable thumbnail
  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (AB210528), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Mouse
Tested
Tested
Tested
Rat
Tested
Expected
Expected
Recombinant fragment - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/120
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

Alternative names

ZIPK, DAPK3, Death-associated protein kinase 3, DAP kinase 3, DAP-like kinase, MYPT1 kinase, Zipper-interacting protein kinase, Dlk, ZIP-kinase

Recommended products

Rabbit Recombinant Monoclonal ZIP Kinase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18809-86
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ZIP Kinase also known as DAPK3 is a serine/threonine protein kinase with a molecular mass of approximately 52 kDa. It is expressed in a variety of tissues including the heart liver and skeletal muscle indicating its broad physiological importance. ZIP Kinase shows activity in phosphorylating specific substrates that influence cell structure and function playing a role in cellular processes.

Biological function summary

ZIP Kinase regulates apoptosis and autophagy impacting cell survival and homeostasis. It does not act in isolation but is part of a complex with other kinases and regulatory proteins. ZIP Kinase interacts with cytoskeletal proteins to mediate cell contraction and motility indicating its involvement in both normal cellular dynamics and stress responses.

Pathways

ZIP Kinase is involved in the apoptosis and autophagy pathways. Within the apoptosis pathway ZIP Kinase partners with proteins like DAPK1 influencing stress and injury responses. In the autophagy pathway it helps in regulating cellular degradation processes. These pathways allow ZIP Kinase to integrate signals that manage cell fate decisions collaboratively modulating cellular life and death.

Associated diseases and disorders

ZIP Kinase associates with hypertension and certain types of cancer. In hypertension ZIP Kinase dysregulation may impact vascular smooth muscle cell contractility due to its connection with myosin light chain phosphorylation. In cancer altered ZIP Kinase activity may affect apoptosis linking it to aberrant cell proliferation. Its interaction with proteins like DAPK1 in both contexts underlines its role in pathological states where cell death and survival are disrupted.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: ZIP Kinase knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: A431 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab210528 observed at 53 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab210528 was shown to specifically react with ZIP Kinase in wild type cells as signal was lost in ZIP Kinase knockout cells. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. ab210528 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Predicted band size: 53 kDa

  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human ZIP Kinase fragment recombinant protein contain aa13-275 with a His-Tag®. Human DAP Kinase 2 fragment recombinant protein contain aa23-285 with a His-Tag®. Human DAP Kinase 1 fragment recombinant protein contain aa13-275 with a His-Tag®. All three recombinant human fragment proteins were made in-house.

    The antibody reacts weakly with DAP kinase 1 and DAP kinase 2; however bands of their appropriate MW (159 kD, 42 kD) are not detected in the tissue lysates.

    All lanes: Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528) at 1/1000 dilution

    Lane 1: Human ZIP Kinase fragment recombinant protein at 0.01 µg

    Lane 2: Human DAP Kinase 2 fragment recombinant protein at 0.01 µg

    Lane 3: Human DAP Kinase 1 fragment recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 29 kDa

    Exposure time: 1s

  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 20124481).

    All lanes: Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Lane 4: Human bladder lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 53 kDa

    Observed band size: 50 kDa

    Exposure time: 3min

  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2, 3, 4, 5, 6 and 7: 30 seconds; Lane 8: 5 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 20124481).

    All lanes: Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: C2C12 (Mouse myoblast cell line) whole cell lysate at 20 µg

    Lane 5: L6 (Rat skeletal muscle cell line) whole cell lysate at 20 µg

    Lane 6: Mouse bladder lysate at 20 µg

    Lane 7: Rat bladder lysate at 20 µg

    Lane 8: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2 and 3: 30 seconds; Lane 4, 5, 6 and 7: 3 minutes; Lane 8, 9, 10 and 11: 30 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 20124481)

    All lanes: Western blot - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse kidney tissue lysate at 10 µg

    Lane 3: Mouse spleen tissue lysate at 10 µg

    Lane 4: Rat brain tissue lysate at 10 µg

    Lane 5: Rat heart tissue lysate at 10 µg

    Lane 6: Rat kidney tissue lysate at 10 µg

    Lane 7: Rat spleen tissue lysate at 10 µg

    Lane 8: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

    Lane 9: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 10: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 11: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa

    Observed band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ZIP Kinase with ab210528 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm and nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:

    -ve control 1: ab210528 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ZIP Kinase with ab210528 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm and nuclear staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:

    -ve control 1: ab210528 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling ZIP Kinase with ab210528 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling ZIP Kinase with ab210528 at 1/600 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    Note:Cells were permeabilised with 90% methanol -1XPBS (-20?, 30min).

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