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AB254566

Anti-ZIP7 antibody

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(3 Publications)

Rabbit Polyclonal ZIP7 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SLC39A7 aa 1-100.

View Alternative Names

HKE4, RING5, SLC39A7, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4, Really interesting new gene 5 protein, Solute carrier family 39 member 7, ZIP7

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZIP7 antibody (AB254566)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZIP7 antibody (AB254566)

Immunocytochemistry/Immunofluorescence analysis of human brain tissue labeling ZIP7 with ab254566 at 20μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP7 antibody (AB254566)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP7 antibody (AB254566)

Immunohistochemical analysis of human brain tissue labeling ZIP7 with ab254566 at 0.5μg/ml.

Western blot - Anti-ZIP7 antibody (AB254566)
  • WB

Supplier Data

Western blot - Anti-ZIP7 antibody (AB254566)

Lane 1:

Western blot - Anti-ZIP7 antibody (ab254566) at 0.5 µg/mL

Lane 2:

Western blot - Anti-ZIP7 antibody (ab254566) at 1 µg/mL

All lanes:

Rat brain tissue at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 50 kDa

false

Western blot - Anti-ZIP7 antibody (AB254566)
  • WB

Supplier Data

Western blot - Anti-ZIP7 antibody (AB254566)

All lanes:

Western blot - Anti-ZIP7 antibody (ab254566) at 0.5 µg/mL

All lanes:

Mouse brain tissue lysate

Predicted band size: 50 kDa

false

Western blot - Anti-ZIP7 antibody (AB254566)
  • WB

Supplier Data

Western blot - Anti-ZIP7 antibody (AB254566)

All lanes:

Western blot - Anti-ZIP7 antibody (ab254566) at 1 µg/mL

Lane 1:

HEK-293 cell lysate at 15 µg

Lane 2:

A549 cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 15 µg

Lane 4:

HepG2 cell lysate at 15 µg

Lane 5:

K562 cell lysate at 15 µg

Lane 6:

MCF7 cell lysate at 15 µg

Lane 7:

SK-N-SH cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 50 kDa

false

Western blot - Anti-ZIP7 antibody (AB254566)
  • WB

Supplier Data

Western blot - Anti-ZIP7 antibody (AB254566)

All lanes:

Western blot - Anti-ZIP7 antibody (ab254566) at 1 µg/mL

Lane 1:

Human brain tissue lysate at 15 µg

Lane 2:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC39A7 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q92504

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC39A6/ZIP-6 antibody [SP300] (AB227696)

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZIP7 also known as SLC39A7 is a zinc transporter protein that plays a role in regulating zinc homeostasis within cells. It encodes a protein weighing approximately 56 kDa. ZIP7 is located on the membrane of the endoplasmic reticulum and the Golgi apparatus. It facilitates the release of stored zinc into the cytosol where zinc acts as an essential cofactor for various cellular processes. Expressions of ZIP7 can be found widely across different tissues with significant levels in the liver brain and pancreas.
Biological function summary

ZIP7 influences intracellular signaling and cellular functions by regulating zinc levels. While not part of a complex ZIP7 is involved in activating protein kinases that require zinc for their function including tyrosine kinases. Increased intracellular zinc levels due to ZIP7 activity can signal the activation of MTF1 a transcription factor important for metal homeostasis. ZIP7 by affecting these signaling cascades participates in critical processes like cellular growth and division.

Pathways

Zinc release through ZIP7 integrates into important signaling pathways like the MAPK and PI3K-AKT pathways. In these pathways ZIP7 interacts with proteins such as ERK and AKT which are vital for cell proliferation and survival. ZIP7's regulation of zinc availability therefore ties it closely to the control of cellular responses that depend on these pathways for growth and differentiation.

Disruptions in ZIP7 function link to conditions such as cancer and neurodegenerative diseases. ZIP7's misregulation has associations with breast cancer where altered zinc signaling influences cancer cell proliferation and metastasis. Furthermore Alzheimer's disease connects to ZIP7 through disturbances in metal homeostasis in neurons which are critical for neuronal function and health. Understanding ZIP7's role in these diseases could provide insights for therapeutic strategies targeting zinc homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transports Zn(2+) from the endoplasmic reticulum (ER)/Golgi apparatus to the cytosol, playing an essential role in the regulation of cytosolic zinc levels (PubMed : 14525538, PubMed : 15705588, PubMed : 28205653, PubMed : 29980658). Acts as a gatekeeper of zinc release from intracellular stores, requiring post-translational activation by phosphorylation, resulting in activation of multiple downstream pathways leading to cell growth and proliferation (PubMed : 22317921, PubMed : 28205653, PubMed : 29980658). Has an essential role in B cell development and is required for proper B cell receptor signaling (PubMed : 30718914). Plays an important role in maintaining intestinal epithelial homeostasis and skin dermis development by regulating ER function (By similarity). Controls cell signaling pathways involved in glucose metabolism in skeletal muscle (By similarity). Has a protective role against ER stress in different biological contexts (PubMed : 29980658, PubMed : 30237509). Mediates Zn(2+)-induced ferroptosis (PubMed : 33608508).
See full target information SLC39A7
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational neuroscience 15:20220334 PubMed38623573

2024

The ACE2/Ang-(1-7)/MasR axis alleviates brain injury after cardiopulmonary resuscitation in rabbits by activating PI3K/Akt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Cheng,Hong Yang,Fang Chen,Li Qiu,Fang Chen,Yanhua Du,Xiangping Meng

Aging 12: PubMed33535184

2021

Evaluation of the prognostic values of solute carrier (SLC) family 39 genes for patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhou,Yaoqi Zhu,Huizhong Qi,Liang Liang,Hao Wu,Jingping Yuan,Qingyong Hu

Bioscience reports 40: PubMed32744318

2020

Analysis of the prognostic significance of solute carrier (SLC) family 39 genes in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Limei Liu,Jiaomin Yang,Chao Wang
View all publications
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