Anti-ZIP8/SLC39A8 antibody [EPR29194-220]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP8/SLC39A8 antibody [EPR29194-220] (AB325498)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ZIP8/SLC39A8 with ab325498 at 1/100 (5.08 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human placenta.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP8/SLC39A8 antibody [EPR29194-220] (AB325498)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling ZIP8/SLC39A8 with ab325498 at 1/100 (5.08 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP8/SLC39A8 antibody [EPR29194-220] (AB325498)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ZIP8/SLC39A8 with ab325498 at 1/100 (5.08 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : nearly no staining on human skeletal muscle.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-ZIP8/SLC39A8 antibody [EPR29194-220] (AB325498)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle (PMID : 12504855).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ZIP8/SLC39A8 antibody [EPR29194-220] (ab325498) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 80 µg

Lane 2:

Human skeletal muscle tissue lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 150 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-ZIP8/SLC39A8 antibody [EPR29194-220] (AB325498)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab325498 was shown to bind specifically to ZIP8/SLC39A8. Target of interest was observed at 150 kDa in wild-type HCT 166 cell lysates (lane 4) with no signal observed at this size in SLC39A8 knockout cell line (lane 5). The lysates were kindly provided by the collaborator.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 31261654).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ZIP8/SLC39A8 antibody [EPR29194-220] (ab325498) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 80 µg

Lane 3:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 80 µg

Lane 4:

Wilde-type HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 80 µg

Lane 5:

SLC39A8 knockout HCT 116 whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,150 kDa,36 kDa

false

Exposure time: 180s