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AB223477

Anti-ZKSCAN3 antibody

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(4 Publications)

Rabbit Polyclonal ZKSCAN3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZKSCAN3 aa 1-300.

View Alternative Names

ZFP47, ZNF306, ZNF309, ZSCAN13, ZKSCAN3, Zinc finger protein with KRAB and SCAN domains 3, Zinc finger and SCAN domain-containing protein 13, Zinc finger protein 306, Zinc finger protein 309, Zinc finger protein 47 homolog, Zf47, Zfp-47

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (AB223477)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (AB223477)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZKSCAN3 (green) using ab223477 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZKSCAN3 antibody (AB223477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZKSCAN3 antibody (AB223477)

Paraffin-embedded human lymph node tissue stained for ZKSCAN3 using ab223477 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ZKSCAN3 antibody (AB223477)
  • WB

Supplier Data

Western blot - Anti-ZKSCAN3 antibody (AB223477)

All lanes:

Western blot - Anti-ZKSCAN3 antibody (ab223477) at 1/500 dilution

Lane 1:

Mouse heart lysate

Lane 2:

Mouse spleen lysate

Lane 3:

Mouse lung lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 61 kDa

Observed band size: 44 kDa,61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZKSCAN3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9BRR0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZKSCAN3 also known as Zinc finger protein with KRAB and SCAN domains 3 is a transcription factor with an approximate mass of 95 kDa. It contains zinc finger motifs that bind to DNA influencing the transcription of specific genes. ZKSCAN3 is expressed in various tissues with high levels noted in the kidney liver and lung. It plays a role in gene regulation by modulating transcriptional responses making it an important factor in cellular function.
Biological function summary

ZKSCAN3 impacts cellular processes and functions as a transcriptional suppressor. ZKSCAN3 has been identified as part of certain protein complexes that interact with chromatin remodelers and co-regulators. This interaction helps in regulating gene expression critical for cell growth and development. Its activity influences various biological processes including cell cycle progression and apoptosis highlighting its role in maintaining cellular homeostasis.

Pathways

ZKSCAN3 serves functions in the autophagy and Wnt signaling pathways. Within the autophagy pathway it negatively regulates autophagic activity impacting cellular homeostasis and survival under stress conditions. ZKSCAN3 is related to proteins like LC3 and p62 which are significant in this pathway. Its interaction within the Wnt signaling pathway contributes to the regulation of gene expression related to proliferation and differentiation with beta-catenin as a notable interacting protein.

ZKSCAN3 shows implications in cancer and neurodegenerative diseases. In cancers its overexpression correlates with tumor progression and poor prognosis often through interaction with proteins such as MYC and mTOR which promote cancer cell survival. Regarding neurodegenerative disorders alterations in ZKSCAN3 expression influence pathways linked with neuronal survival and autophagy affecting disease mechanisms in disorders such as Alzheimer's disease. These connections make ZKSCAN3 an interesting target for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.
See full target information ZKSCAN3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 30:1764-1776 PubMed38617741

2024

suppresses autophagic flux N6-methyladenosine demethylation of mRNA in acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Shuai Zhu,Geng-Wen Huang

International journal of molecular sciences 23: PubMed36012568

2022

Role of the WNT/β-catenin/ZKSCAN3 Pathway in Regulating Chromosomal Instability in Colon Cancer Cell lines and Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Eun Cho,Jeong-Hee Kim,Young-Hyun Che,Yong-Jun Kim,Ji-Youn Sung,Yoon-Wha Kim,Bong-Geun Choe,Sun Lee,Jae-Hoon Park

BMC medicine 20:189 PubMed35610640

2022

Role of EZH2-mediated H3K27me3 in placental ADAM12-S expression: implications for fetoplacental growth.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Nan Zhu,Xiao-Wen Gan,Fan Pan,Xiao-Tian Ni,Leslie Myatt,Wang-Sheng Wang,Kang Sun

Cancer cell international 20:216 PubMed32518525

2020

ZKSCAN3 drives tumor metastasis via integrin β4/FAK/AKT mediated epithelial-mesenchymal transition in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqiong Li,Nan Hao,Juan Han,Mi Zhang,Xiaomei Li,Nan Yang
View all publications
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