Rabbit Polyclonal ZKSCAN3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZKSCAN3 aa 1-400.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZKSCAN3 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRR0
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.
Alternative names
ZFP47, ZNF306, ZNF309, ZSCAN13, ZKSCAN3, Zinc finger protein with KRAB and SCAN domains 3, Zinc finger and SCAN domain-containing protein 13, Zinc finger protein 306, Zinc finger protein 309, Zinc finger protein 47 homolog, Zf47, Zfp-47
Recommended products
Rabbit Polyclonal ZKSCAN3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZKSCAN3 aa 1-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZKSCAN3 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRR0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZKSCAN3 also known as Zinc finger protein with KRAB and SCAN domains 3 is a transcription factor with an approximate mass of 95 kDa. It contains zinc finger motifs that bind to DNA influencing the transcription of specific genes. ZKSCAN3 is expressed in various tissues with high levels noted in the kidney liver and lung. It plays a role in gene regulation by modulating transcriptional responses making it an important factor in cellular function.
- Biological function summary
ZKSCAN3 impacts cellular processes and functions as a transcriptional suppressor. ZKSCAN3 has been identified as part of certain protein complexes that interact with chromatin remodelers and co-regulators. This interaction helps in regulating gene expression critical for cell growth and development. Its activity influences various biological processes including cell cycle progression and apoptosis highlighting its role in maintaining cellular homeostasis.
- Pathways
ZKSCAN3 serves functions in the autophagy and Wnt signaling pathways. Within the autophagy pathway it negatively regulates autophagic activity impacting cellular homeostasis and survival under stress conditions. ZKSCAN3 is related to proteins like LC3 and p62 which are significant in this pathway. Its interaction within the Wnt signaling pathway contributes to the regulation of gene expression related to proliferation and differentiation with beta-catenin as a notable interacting protein.
- Associated diseases and disorders
ZKSCAN3 shows implications in cancer and neurodegenerative diseases. In cancers its overexpression correlates with tumor progression and poor prognosis often through interaction with proteins such as MYC and mTOR which promote cancer cell survival. Regarding neurodegenerative disorders alterations in ZKSCAN3 expression influence pathways linked with neuronal survival and autophagy affecting disease mechanisms in disorders such as Alzheimer’s disease. These connections make ZKSCAN3 an interesting target for therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-ZKSCAN3 antibody (ab96731)
7.5% SDS PAGE
All lanes: Western blot - Anti-ZKSCAN3 antibody (ab96731) at 1/1000 dilution
Lane 1: HeLa S3 whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (ab96731)
Immunofluorescence analysis of ZNF306 in paraformaldehyde fixed HeLa, using ab96731 at a 1/200 dilution.
Lower image (2): merged with DNA probe.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com