AB169541

Anti-Zmat2 antibody [EPR11136]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ZMAT2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Zinc finger matrin-type protein 2, ZMAT2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] (AB169541)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] (AB169541)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Zmat2 with ab169541 antibody at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] (AB169541)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Zmat2 with ab169541 antibody at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Zmat2 antibody [EPR11136] (AB169541)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Zmat2 antibody [EPR11136] (AB169541)

Immunofluorescent analysis of HeLa cells labeling Zmat2 with ab169541 at 1/250.

Unknown

Western blot - Anti-Zmat2 antibody [EPR11136] (AB169541)

All lanes:

Western blot - Anti-Zmat2 antibody [EPR11136] (ab169541) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11136

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zmat2 also known as Zinc finger matrin-type protein 2 plays a role in RNA processing. This protein has a mass of approximately 37 kDa. It shows expression in most tissues with higher levels in certain areas of the brain and reproductive organs. The protein contains a zinc finger domain which facilitates binding to RNA and possibly other molecular components suggesting its involvement in cellular processes requiring precise regulation.

Biological function summary

Zmat2 influences the maturation and modification of mRNA. It serves as part of the spliceosome complex which is responsible for the splicing of pre-mRNA and is essential for the regulation of gene expression. Zmat2 contributes to the appropriate removal of introns and joining of exons ensuring the production of functional RNA transcripts. Its actions are important for the maintenance of cellular function and response to environmental cues.

Pathways

Several components in the RNA splicing machinery are associated with Zmat2. Among these the spliceosome assembly and mRNA surveillance pathways are notably significant. Zmat2 works in conjunction with PRPF8 and several small nuclear ribonucleoproteins (snRNPs) which are necessary for the assembly of the spliceosomal complex. These interactions ensure effective and reliable mRNA processing and turnover.

Abnormal functioning of Zmat2 may have a connection with certain neurological conditions. Disruption in RNA splicing can lead to the accumulation of faulty proteins potentially resulting in diseases like amyotrophic lateral sclerosis (ALS) and some neurodegenerative disorders. The involvement of Zmat2 in pathways with other proteins like FUS and TDP-43 which are linked to these conditions highlights its importance in maintaining neural health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing as a component of the spliceosome.

See full target information ZMAT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 22:3477-3489 PubMed40860777

2025

NDUFS1 upregulates ENaCα by NAD+ to promote alveolar fluid clearance in acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Wang,Mengting Chen,Jianping Zhu,Yu Zhang,Jian Lu,Zhiying Yue,Zhengfeng Yang,Ruilan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zmat2 antibody [EPR11136]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] (AB169541)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] (AB169541)

Immunocytochemistry/ Immunofluorescence - Anti-Zmat2 antibody [EPR11136] (AB169541)

Western blot - Anti-Zmat2 antibody [EPR11136] (AB169541)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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